Process for extracting and purifying moringa oleifera polysaccharide

A kind of Moringa polysaccharide and technology, applied in the field of extraction of active ingredients of natural products, can solve problems such as unfavorable industrialized production, low product processing performance, low extraction purity of Moringa polysaccharide, etc., achieves good processing and service performance, and low cost of raw materials , the effect of high purity

Inactive Publication Date: 2018-01-12
隆安县荣胜养殖有限公司
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a process for extracting and purifying Moringa polysaccharides to solve the current problems of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] A process for extracting and purifying Moringa polysaccharides includes the following steps:

[0025] (1) Preparation: Take the Moringa oleifera leaves in the production period of 45 days, dry, crush, and pass through a 50-mesh sieve to prepare Moringa oleifera leaf powder;

[0026] (2) Delipidation and sugar removal: The Moringa leaf powder in step (1) was extracted with 8 times the amount of petroleum ether and 8 times the amount of 80% ethanol in turn at 80°C under reflux extraction for 2 hours, and then filtered to obtain Degreasing and removing the filter residue of monosaccharides and polysaccharides;

[0027] (3) Extraction: Add 10 times the amount of sterile distilled water to the filter residue in step (2), and extract 3 times under microwave heating and reflux at 85°C, each extraction is 10 minutes, the extract is vacuum filtered, and the concentrated volume is 45%. Concentrate, dissolve the primary concentrate again with 6 times the volume of ster...

Example Embodiment

[0030] Example 2

[0031] (1) Material preparation: take the Moringa oleifera leaves in the production period of 46 days, dry, crush, and pass through a 50-mesh sieve to prepare Moringa oleifera leaf powder;

[0032] (2) Delipidation and sugar removal: The Moringa leaf powder in step (1) was extracted with 9 times the amount of petroleum ether and 9 times the amount of 80% ethanol in turn at 80°C for 2 times of refluxing, each extraction for 2 hours, and filtration to obtain Degreasing and removing the filter residue of monosaccharides and polysaccharides;

[0033] (3) Extraction: Add 12 times the amount of sterile distilled water to the filter residue in step (2) and extract 3 times under microwave heating and reflux at 88°C, each extraction 12 minutes, the extract is vacuum filtered, and the concentrated volume is 48%. Concentrate, dissolve the primary concentrate again with 7 times the volume of sterile distilled water at 50°C, and extract twice with anhydrous ethanol solvent. Th...

Example Embodiment

[0036] Example 3

[0037] (1) Material preparation: take Moringa leaves with a production period of 50 days, dry, pulverize, and pass through a 50-mesh sieve to prepare Moringa leaf powder;

[0038] (2) Delipidation and sugar removal: The Moringa leaf powder in step (1) was extracted twice with 10 times the amount of petroleum ether and 10 times the amount of 80% ethanol under reflux at 80°C for 3 hours each time, and filtered to obtain Degreasing and removing the filter residue of monosaccharides and polysaccharides;

[0039] (3) Extraction: Add 15 times the amount of sterile distilled water to the filter residue in step (2) and extract 3 times under microwave heating and reflux at 90°C for 15 minutes each time. The extract is vacuum filtered and the concentrated volume is 50%. Concentrate, dissolve the primary concentrate again with 8 times the volume of sterile distilled water at 55°C, extract 3 times with anhydrous ethanol solvent, and concentrate the chromatographic solution at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a process for extracting and purifying moringa oleifera polysaccharide. The process comprises the following steps: preparing dried moringa oleifera leaf powder, performing backflow extraction twice at 80 DEG C in the presence of petroleum ether and 80% ethanol, and filtering so as to obtain degreased filter residue of which monosaccharide and polysaccharide are removed; putting distilled water 10-15 times the filter residue, performing backflow extraction for three times at 85-90 DEG C, performing vacuum filtration on an extract, concentrating, extracting, and further concentrating; mixing a concentrated liquid with n-butyl alcohol and chloroform in a ratio of 1:1:4, oscillating for 15-20 minutes in a rotary oscillator, performing centrifugal separation to remove protein and a chloroform layer, and collecting centrifugate; and decoloring the centrifugate with activated carbon, concentrating, heating concentrated liquid to 60-75 DEG C, and rapidly cooling to 38-40 DEG C, thereby obtaining cured moringa oleifera polysaccharide. Moringa oleifera polysaccharide extracted by using the process disclosed by the invention is high in extraction rate and purity, has thermal reversibility and is good in processing and use performance.

Description

【Technical field】 [0001] The invention belongs to the technical field of extracting active ingredients of natural products, and in particular relates to a process for extracting and purifying Moringa oleifera polysaccharides. 【Background technique】 [0002] Moringa belongs to Moringaceae and Moringa. It is a perennial woody plant. It is a tropical plant with unique economic value. It is mainly distributed in Yunnan, Guangdong, Guangxi, Hainan, Taiwan and other provinces. As a vegetable, food and medicinal material, Moringa has been widely used in improving nutrition, dietotherapy and health care, and medicine and health care. It is known as "the tree of life, the tree of magic". The polysaccharides contained in Moringa can activate cells and enhance immunity, help secrete insulin and regulate blood sugar, and have rich health care effects. Long-term use has obvious effects on lowering high blood pressure, lowering blood fat, and lowering blood sugar. It can also prevent Can...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00
Inventor 马云川
Owner 隆安县荣胜养殖有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap