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Process for extracting and purifying moringa oleifera polysaccharide

A kind of Moringa polysaccharide and technology, applied in the field of extraction of active ingredients of natural products, can solve problems such as unfavorable industrialized production, low product processing performance, low extraction purity of Moringa polysaccharide, etc., achieves good processing and service performance, and low cost of raw materials , the effect of high purity

Inactive Publication Date: 2018-01-12
隆安县荣胜养殖有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a process for extracting and purifying Moringa polysaccharides to solve the current problems of low extraction purity of Moringa polysaccharides, low product processing performance, and unfavorable industrial production.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] A process for extracting and purifying Moringa polysaccharides, comprising the following steps:

[0025] (1) raw material preparation: get the leaf of Moringa oleifera at 45d, dry, pulverize, cross 50 mesh sieves, produce leaf of Moringa oleifera powder;

[0026] (2) Degreasing and desugaring: the Moringa oleifera leaf powder in the step (1) is refluxed and extracted twice at 80°C with 8 times the amount of sherwood oil and 8 times the amount of 80% ethanol successively, and extracted for 2 hours each time, filtered to obtain Degreasing and removal of filter residues of monosaccharides and polysaccharides;

[0027] (3) Extraction: Add 10 times the amount of sterile distilled water to the filter residue in step (2), heat and reflux under microwave heating at 85°C for 3 times, extract for 10 minutes each time, vacuum filter the extract, and concentrate the volume to 45% to obtain once Concentrated solution, the primary concentrated solution was redissolved with 6 times t...

Embodiment 2

[0031] (1) material preparation: get the leaf of Moringa oleifera at 46d in production period, dry, pulverize, cross 50 mesh sieves, produce leaf of Moringa oleifera powder;

[0032] (2) Degreasing and desugaring: the Moringa oleifera leaf powder in step (1) is refluxed and extracted twice at 80°C with 9 times the amount of sherwood oil and 9 times the amount of 80% ethanol successively, and extracted for 2 hours each time, filtered to obtain Degreasing and removal of filter residues of monosaccharides and polysaccharides;

[0033] (3) Extraction: Add 12 times the amount of sterile distilled water to the filter residue in step (2), heat and reflux under microwave heating at 88 ° C for 3 times, extract 12 min each time, vacuum filter the extract, and concentrate the volume to 48% to obtain once Concentrated solution, the primary concentrated solution was re-dissolved with 7 times the volume of sterile distilled water at 50°C, extracted twice with anhydrous ethanol solvent, the ...

Embodiment 3

[0037] (1) raw material preparation: get the leaf of Moringa oleifera at 50d in production period, dry, pulverize, cross 50 mesh sieves, produce leaf of Moringa oleifera powder;

[0038] (2) Degreasing and desugaring: the Moringa oleifera leaf powder in step (1) is refluxed and extracted twice at 80°C with 10 times the amount of sherwood oil and 10 times the amount of 80% ethanol successively, each extraction for 3h, filtered to obtain Degreasing and removal of filter residues of monosaccharides and polysaccharides;

[0039] (3) Extraction: Add 15 times the amount of sterile distilled water to the filter residue in step (2), heat and reflux under microwave heating at 90°C for 3 times, extract for 15 minutes each time, vacuum filter the extract, and concentrate the volume to 50% to obtain once Concentrated solution, the primary concentrated solution is redissolved with 8 times the volume of sterile distilled water at 55°C, extracted 3 times with anhydrous ethanol solvent, the c...

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PUM

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Abstract

The invention discloses a process for extracting and purifying moringa oleifera polysaccharide. The process comprises the following steps: preparing dried moringa oleifera leaf powder, performing backflow extraction twice at 80 DEG C in the presence of petroleum ether and 80% ethanol, and filtering so as to obtain degreased filter residue of which monosaccharide and polysaccharide are removed; putting distilled water 10-15 times the filter residue, performing backflow extraction for three times at 85-90 DEG C, performing vacuum filtration on an extract, concentrating, extracting, and further concentrating; mixing a concentrated liquid with n-butyl alcohol and chloroform in a ratio of 1:1:4, oscillating for 15-20 minutes in a rotary oscillator, performing centrifugal separation to remove protein and a chloroform layer, and collecting centrifugate; and decoloring the centrifugate with activated carbon, concentrating, heating concentrated liquid to 60-75 DEG C, and rapidly cooling to 38-40 DEG C, thereby obtaining cured moringa oleifera polysaccharide. Moringa oleifera polysaccharide extracted by using the process disclosed by the invention is high in extraction rate and purity, has thermal reversibility and is good in processing and use performance.

Description

【Technical field】 [0001] The invention belongs to the technical field of extracting active ingredients of natural products, and in particular relates to a process for extracting and purifying Moringa oleifera polysaccharides. 【Background technique】 [0002] Moringa belongs to Moringaceae and Moringa. It is a perennial woody plant. It is a tropical plant with unique economic value. It is mainly distributed in Yunnan, Guangdong, Guangxi, Hainan, Taiwan and other provinces. As a vegetable, food and medicinal material, Moringa has been widely used in improving nutrition, dietotherapy and health care, and medicine and health care. It is known as "the tree of life, the tree of magic". The polysaccharides contained in Moringa can activate cells and enhance immunity, help secrete insulin and regulate blood sugar, and have rich health care effects. Long-term use has obvious effects on lowering high blood pressure, lowering blood fat, and lowering blood sugar. It can also prevent Can...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 马云川
Owner 隆安县荣胜养殖有限公司
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