Aspergillus strain capable of achieving high-yield production of protease and application of aspergillus strain in soy sauce
A technology of Aspergillus oryzae and protease, which is applied in the field of microorganisms, can solve the problems of low protein decomposition ability and low screening efficiency, and achieve the effects of high screening efficiency, improved utilization rate, and improved protein utilization rate
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Problems solved by technology
 Example 1:
 1. Growth curve drawing:
 Transfer Aspergillus oryzae from the slope of the test tube to the PDA plate, and incubate at 28°C for 72 hours. Connect 3 loops of activated Aspergillus oryzae with an inoculation loop to a triangular flask containing sterilized 12°wort, and pick the tricyclic Aspergillus oryzae spores from the preservation slope into a 10mL sterile wort medium In a test tube, shake well to make a spore suspension, which is cultured and activated in a 28°C incubator for 24 hours. Pour 10 mL of bacterial suspension into an Erlenmeyer flask containing 300 mL of sterile wort culture medium, shake well to make a spore suspension, and culture it on a shaker in an incubator at 28°C.
 Take 14 2ml centrifuge tubes, weigh them and record the weight of the centrifuge tubes to the nearest 0.0001g. Pipette 1ml of 12°wort and 200ul of 1.5×10 5 The activated bacteria solution was placed on a shaker at 28°C for culture. At 0, 4, 8, 12, 16, 20...
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