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Method for treating high-salt phenolic wastewater by utilizing micrococcus luteus enzymic preparation to enhance microorganisms

A microbial treatment, Luteus luteus technology, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve problems such as low degradation efficiency, low bacterial activity, and slow growth rate , to achieve the effect of promoting growth and reproduction, low cost and easy operation

Active Publication Date: 2018-01-16
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE +1
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method of using coccus luteus enzyme preparation to strengthen microbial treatment of high-salt and phenol-containing wastewater in view of the shortcomings of low bacterial activity, slow growth rate and low degradation efficiency in the existing biological treatment system of high-salt and phenol-containing wastewater. Phenol Wastewater Method

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  • Method for treating high-salt phenolic wastewater by utilizing micrococcus luteus enzymic preparation to enhance microorganisms
  • Method for treating high-salt phenolic wastewater by utilizing micrococcus luteus enzymic preparation to enhance microorganisms

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Embodiment Construction

[0025] The present invention utilizes coccus luteus enzyme preparation to strengthen the method for microbial treatment of high-salt phenol-containing wastewater, comprising the following steps:

[0026] 1. Preparation of luteus enzyme preparation

[0027] Activation culture of coccus luteus: the used coccus luteus (Micrococcus Luteus IAM14879) can be purchased from the Micrococcus Luteus Culture Collection Center of RIKEN. LMM medium (4.0g / L NH 4 Cl, 1.4g / L KH 2 PO 4, 0.005g / L biotin, 0.02g / L L-methionine, 0.04g / L vitamin B1, 1.0g / L inosine, 0.03g / L MgSO 4 , 8.75g / L L-lithium lactate, 1.5ml / L mineral salt solution (0.375g / L CuSO 4 ·5H 2 O, 0.785g / L MnCl 2 4H 2 O, 0.18g / L FeSO 4 ·7H 2 O, 0.029g / L Na 2 MoO 4 2H 2 O, 0.089g / L ZnSO 4 ·7H 2 (0), pH is 7.5) into the ampoule containing the luteus powder, left at room temperature for 20min, and streaked onto the LMM solid plate with an inoculation loop.

[0028] Fermentation culture of Luteus luteus: Pick one loop of l...

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Abstract

The invention discloses a method for treating high-salt phenolic wastewater by utilizing micrococcus luteus enzymic preparation to enhance microorganisms. The method comprises the following steps: firstly domesticating active sludge by utilizing enzymic preparation, and then treating the high-salt phenolic wastewater by collectively utilizing the enzymic preparation. The enzymic preparation can accelerate the domestication speed of the active sludge and can improve the performance of the active sludge, thereby enhancing the efficacy of the microorganisms for treating the wastewater, and prolonging the service life of a membrane, specifically as follows: the tolerance degree of the active sludge to the salt is increased from 100g / L to 180g / L, the tolerance degree to the phenol is increasedfrom 2500 mg / L to 3500 mg / L, the relative abundance of the flora of actinobacteria and bacteroidetes in the active sludge is increased, the decomposition rate of the phenol in MBR is increased by 40 to 60 percent, and the membrane pollution period is prolonged by 8 to 10 days. Therefore, the micrococcus luteus enzymic preparation not only can be used for rapidly domesticating the active sludge, but also can be used for improving the activity of the functional flora in the wastewater biological treatment system, can prolong the service life of the membrane, and provides a safe, cheap and efficient method for promoting the popularization and application of the microorganism technique and MBR in treating complicated industrial wastewater.

Description

technical field [0001] The invention belongs to the field of industrial wastewater treatment, and in particular relates to a method for using coccus luteus enzyme preparation to strengthen microorganisms to treat high-salt phenol-containing wastewater. Background technique [0002] Phenolic substances belong to aromatic compounds, and the main emission types include phenol, chlorophenol and p-nitrophenol, which are toxic and harmful pollutants that are difficult to degrade in chemical production. Phenolic compounds have been listed as one of 129 priority pollutants and 65 toxic pollutants due to their wide variety, refractory degradation, and “three-inducing” effects. In recent years, with the rapid development of industries such as petrochemical, pharmaceutical, building materials, pesticides, papermaking, gas, electronics, textiles, dyes, plastics, and coking, the discharge of phenolic wastewater has greatly increased, and it has gradually become one of the main pollution ...

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Application Information

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IPC IPC(8): C02F3/34C02F101/34
Inventor 梅荣武苏晓梅张宇张云鸽林红军陈建荣
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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