Blood protein responsive type gamma-PGA (polyglutamic acid) hydrogel hemostasis material as well as preparation method and application thereof
A technology of polyglutamic acid and blood protein, applied in application, medical science, surgery, etc., can solve the problems of poor blood responsiveness and poor integration ability of wet bleeding wounds, achieve high hemostasis efficiency and solve poor integration ability of wet bleeding wounds , good biocompatibility
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Embodiment 1
[0038] Dissolve γ-polyglutamic acid (γ-PGA, molecular weight 100,000 Daltons) in a mixed solution of MES buffer (pH=4.8, 0.1M) and methanol (MES / MeOH=1 / 1, v / v), the mass volume ratio of γ-PGA to the mixed solution is 10g / L, stir and mix evenly; then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) And N-hydroxysuccinimide (NHS), stirred and activated in ice bath for 2h. Add 3-phenylpropanamide (PHPA) and dopamine (DA) respectively, and stir the reaction for 5 hours at room temperature; the molar ratio of each substance is as follows, EDC:γ-PGA (-COOH)=1:1, EDC:NHS=1 :1, γ-PGA(-COOH):PHPA=1:1, γ-PGA(-COOH):DA=1:1. The obtained system was transferred to a dialysis bag, and placed in deionized water for dialysis for 3 days; the purified solution obtained after dialysis was freeze-dried to obtain γ-polyglutamic acid-3-phenylpropanamide-dopamine (γ-PGA-PHPA -DA) polymer, PHPA and DA grafting rates were 6% and 11%, respectively.
Embodiment 2
[0040]Dissolve γ-PGA (molecular weight: 300,000 Daltons) in a mixed solution of MES buffer (pH=5.0, 0.05M) and methanol (MES / MeOH=1 / 1, v / v), γ-PGA and The mass volume ratio of the mixed solution is 10g / L, stir and mix evenly; then add EDC and NHS, stir and activate in ice bath for 3h. Add PHPA and DA respectively, stir and react at room temperature for 10 h; the molar ratio of each substance is as follows, EDC: γ-PGA (-COOH) = 2: 1, EDC: NHS = 1: 1, γ-PGA (-COOH): PHPA=1:2, γ-PGA(-COOH):DA=1:1. The obtained system was transferred to a dialysis bag, and placed in deionized water for dialysis for 5 days; the purified solution obtained after dialysis was freeze-dried to obtain a γ-PGA-PHPA-DA polymer, and the grafting rates of PHPA and DA were 13% and 10%, respectively. 9%.
Embodiment 3
[0042] Dissolve γ-PGA (molecular weight: 700,000 Daltons) in a mixed solution of MES buffer (pH=5.5, 0.12M) and methanol (MES / MeOH=1 / 1, v / v), γ-PGA and The mass volume ratio of the mixed solution is 15g / L, stir and mix evenly; then add EDC and NHS, stir and activate in ice bath for 6h. Add PHPA and DA respectively, stir and react at room temperature for 8h; the molar ratio of each substance is as follows, EDC:γ-PGA(-COOH)=3:1, EDC:NHS=1:1,γ-PGA(-COOH): PHPA=1:3, γ-PGA(-COOH):DA=1:1. The obtained system was transferred to a dialysis bag, placed in deionized water and dialyzed for 7 days; the obtained purified solution after dialysis was freeze-dried to obtain a γ-PGA-PHPA-DA polymer, and the grafting rates of PHPA and DA were 17% and 10%, respectively. 6%.
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