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Preparation method of edible mushroom bioactive peptide

A technology for edible fungi and active peptides, which is applied to medical preparations containing active ingredients, antibacterial drugs, pharmaceutical formulations, etc., can solve problems such as waste of raw materials, human body absorption and utilization rate, etc., to improve immunity and food safety. , the effect of diuretic and moisturizing activity

Inactive Publication Date: 2018-01-30
赵德润
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the waste of raw materials in the extraction process of various edible fungus active substances in the prior art and the shortcomings of low absorption and utilization by the human body, the present invention provides a preparation method of edible fungus active peptides, which are rich in biological Active peptides have the functions of nourishing, improving the body's immunity, anti-tumor, anti-virus, anti-bacterial and anti-inflammatory, prevention and auxiliary treatment of cardiovascular and cerebrovascular diseases, regulating endocrine, protecting liver and liver, clearing heat and detoxification, calming the nerves, removing blood stasis and regulating qi, moisturizing the lungs and eliminating Phlegm, diuretic and damp, anti-cancer and other pharmacological activities, and easy to digest and absorb, rich in nutrients and high utilization rate of the body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1. 1. Preparation of edible fungus active peptide liquid: select 10 kg of freshly dried Cordyceps sinensis of the year for pulverization; add water 10 times the weight of the pulverized edible fungus, dilute and soak for 30 minutes and stir evenly, and the edible fungus after homogenization is broken Adjust the pH value to 6, add cellulase, pectinase, neutral protease, papain (1:1:1:2) multi-enzyme for enzymolysis, the amount of enzyme added is 9500 enzyme activity units per gram of edible fungus (u ), the temperature is controlled at 50 degrees Celsius, and the enzymolysis is carried out for 4.5 hours; after the enzymolysis is completed, the enzyme is deactivated in a boiling water bath for 10 minutes, then cooled and centrifuged, and the speed of the centrifuge is about 9000rpm, and the active peptide liquid of Cordyceps sinensis is obtained;

[0017] 2. Put the Cordyceps sinensis active peptide solution prepared above into a stainless steel barrel and put i...

Embodiment 2 1

[0020] Embodiment two, 1, the preparation of active peptide liquid of edible fungus: choose the clean Grifola frondosa fungus 25kg that is fresh in that year and carry out pulverizing treatment; add the water of 5 times of the weight of pulverized edible fungus and carry out diluting and soaking for 30 minutes and stir evenly, after breaking and homogenizing Adjust the pH of the Grifola frondosa fungus liquid to 4, add cellulase, edible fungus enzyme, neutral protease, papain (1:1:1:2) for enzymatic hydrolysis, and the enzyme dosage is 10,000 per gram of edible fungus Enzyme activity unit (u), temperature control 55 degrees Celsius, enzymolysis for 6 hours; after enzymolysis, boiling water heat bath to kill enzyme for 15 minutes, cooling and centrifuging, centrifuge speed is about 8000rpm, and the active peptide liquid of Grifola frondosa is obtained.

[0021] 2. Put the Grifola frondosa active peptide liquid prepared above into a stainless steel barrel and put it into a steam ...

Embodiment 3 1

[0024] Embodiment three, 1, preparation of active peptide liquid of edible fungus: select 15 kg of fresh dried morels of the year and carry out crushing treatment; add water 15 times the weight of ultrafine pulverized edible fungi, dilute and soak for 30 minutes, stir evenly, break and homogenate Adjust the pH value of the final hickory chick liquid to 3.5, add cellulase, edible fungus enzyme, neutral protease, papain (1:1:1:2) for enzymatic hydrolysis, and the enzyme dosage is 8000 per gram of edible fungus Enzyme activity unit (u), temperature control 58 degrees Celsius, enzymatic hydrolysis for 4.5 hours; after enzymolysis, boiling water heat bath to inactivate the enzyme for 12 minutes, cooling and centrifuging, the centrifuge speed is about 6000rpm, and the morel active peptide liquid is obtained.

[0025] 2. Put the morel active peptide liquid prepared above into a stainless steel barrel and put it into a steam sterilizer for 25 minutes of steam autoclaving at 115 degrees...

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Abstract

The invention is named as preparation method of edible mushroom bioactive peptide. The invention relates to the field of traditional Chinese medicine and biotechnology. The preparation method includessteps of taking one or several fresh or dry edible mushroom as a raw material; and carrying out the operations of smashing, homogenizing, enzymolysis, centrifugation, sterilization and ultrafiltration. The method further includes steps of adjusting pH value of the broken and homogenized edible mushroom to 3-6; adding cellulose, edible mushroom enzyme, neutral protease, and papain at the ratio of1: 1: 1: 2 to perform enzymolysis; adding 5000-1000 pieces of enzyme activity units (u) to every gram of edible mushrooms; performing enzymolysis at 60-60 DEG C for 3-6 hours; then performing enzyme deactivation, centrifugation, sterilization and ultrafiltration to obtain the edible mushroom bioactive peptide fluid with molecular weight of 5000 Daltons below. The edible mushroom bioactive peptidecan be used for preparing freeze-dried powder, oral liquid and capsule products. The edible mushroom bioactive peptide has the pharmacological activities of nourishing, improving immunity of the organism, preventing tumor and virus, antisepsis and anti-inflammation, preventing and helping to treat cardiovascular and cerebrovascular diseases, tranquilizing and allaying excitement, moistening lung and eliminating phlegm, preventing and treating cancer, and others. The preparation method is scientific and strict in procedure, strong in operability and easy to perform industrial production.

Description

technical field [0001] The invention relates to the fields of traditional Chinese medicine and biotechnology. In particular, it relates to an anti-tumor, anti-virus, anti-bacterial and anti-inflammatory, prevention and auxiliary treatment of cardiovascular and cerebrovascular diseases, endocrine regulation, liver protection, heat-clearing and detoxification, calming and tranquilizing, removing blood stasis and regulating qi, moistening The preparation method of the active peptide of edible fungus which has the functions of removing phlegm from the lung, diuresis and dampness, etc. Background technique [0002] Edible fungus refers to the general term for fungi that can be used by humans, including shiitake mushrooms, boletus, fungus, white fungus, throat fungus, Cordyceps flower, bamboo fungus, tea tree mushroom, etc. , Grifola frondosa, Morchella, etc., some wild mushrooms that have nourishing medicinal effects also belong to the fungus category. According to statistics, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61P1/14A61P37/04A61P35/00A61P31/04A61P5/00A61P1/16A61P25/20A61P11/10A61P39/00C12P21/06A61K36/076A61K36/074A61K36/07A61K36/068A61K36/062
Inventor 赵德润
Owner 赵德润
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