Synergic disease-resistant bacterium as well as culture method and applications thereof
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A cultivation method and anti-bacteria technology, applied in the field of microorganisms, can solve the problems of small use area, high cost, inability to apply antibacterial pesticides, etc., and achieve the effects of high safety, reduced usage, and improved control effect.
Active Publication Date: 2018-01-30
SHANGHAI NONGLE BIOLOGICAL PROD
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[0002] The control of fungal diseases such as rice, vegetables, and fruits is currently dominated by chemical pesticides, which have brought many adverse effects on the environment and the human body. Shenzimycin is a new type of biopesticide with high efficiency and low toxicity. High, resulting in a relatively small area of use, how to improve the control of Shenzimycin on rice sheath blight and other fungal diseases, reduce the cost of use, and develop new preparations so that it can be more widely used in crop production It has become the research direction of technicians
[0003] At present, some bacillus are sold as a kind of biological pesticides on the market, but it is still difficult to achieve the desired effect, and it cannot be applied together with some antibacterial pesticides
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Embodiment 1
[0029] The screening and cultivation scheme of disease-resistant and synergistic Bacillus is as follows:
[0030] Starting strain: SD23 Bacillus Cereus
[0031] Slant, plate culture medium:
[0032] 5 grams of beef extract, 10 grams of peptone, 5 grams of chloride, 10-15 grams of agar, 1000 ml of water, PH7.0-7.5, sterilized at 121 ° C for 20 minutes
[0033] Shake flask medium: 30 grams of soybean meal powder, 10 grams of corn flour, 3 grams of peptone, 5 grams of yeast powder, and a fermentation temperature of 32°C.
[0034] Shaker: Oscillating
[0035] (1) Inoculate the starting strain SD23 on the slant medium, culture at 32°C for 2-3 days, wash down the slant spores, and make a bacterial suspension;
[0036] Breeding method:
[0037] (1) Natural separation
[0038] Preparation of the single spore suspension of the strain: wash the cultivated slant with 0.85% physiological saline in a triangular flask with glass beads, shake for several minutes, filter through a steril...
Embodiment 2
[0097] This embodiment is basically the same as Embodiment 1, except that the application rate of the compound agent in this embodiment is 30 g / mu.
Embodiment 3
[0099] This embodiment is basically the same as Embodiment 1, except that the application rate of the compound agent in this embodiment is 80 g / mu.
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Abstract
The invention relates to a synergic disease-resistant bacterium as well as a culture method and applications of the synergic disease-resistant bacterium. The synergic disease-resistant bacterium is Bacillus safensis, and is assigned with the microbial accession number of CGMCC No.7847. The culture method comprises the following steps: selecting an SD23 strain as a starting strain for screening ofa first round, preparing a bacterium suspension, sequentially carrying out natural separation screening, mutation treatment screening and culture and screening of high-concentration shenqinmycin culture medium, wherein the strain obtained by culture and screening of the high-concentration shenqinmycin culture medium is adopted as a starting strain for screening of the next round, and repeatedly carrying out the process for multiple rounds, thus obtaining the target strain. Compared with the prior art, the synergic disease-resistant bacterium has the advantages that the synergic disease-resistant bacterium has good drug resistance to shenqinmycin, and forms a mixed preparation with shenqinmycin, the use is safe, the prevention and treatment effects for rice sheath blight disease and watermelon fusarium wilt can be improved, the yield is increased, meanwhile, the use amount of shenqinmycin is reduced, so that the cost is reduced.
Description
technical field [0001] The invention relates to the field of microbes, in particular to a synergistic anti-disease bacterium and its cultivation method and application. Background technique [0002] The control of fungal diseases such as rice, vegetables, and fruits is currently dominated by chemical pesticides, which have brought many adverse effects on the environment and the human body. Shenzimycin is a new type of biopesticide with high efficiency and low toxicity. High, resulting in a relatively small area of use, how to improve the control of Shenzimycin on rice sheath blight and other fungal diseases, reduce the cost of use, and develop new preparations so that it can be more widely used in crop production Among them, it has become the research direction of technicians. [0003] At present, some bacillus are sold as a kind of biological pesticides in the market, but it is still relatively difficult to achieve the desired effect, and it cannot be applied together wi...
Claims
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