Synergic disease-resistant bacterium as well as culture method and applications thereof

Abstract

The invention relates to a synergic disease-resistant bacterium as well as a culture method and applications of the synergic disease-resistant bacterium. The synergic disease-resistant bacterium is Bacillus safensis, and is assigned with the microbial accession number of CGMCC No.7847. The culture method comprises the following steps: selecting an SD23 strain as a starting strain for screening ofa first round, preparing a bacterium suspension, sequentially carrying out natural separation screening, mutation treatment screening and culture and screening of high-concentration shenqinmycin culture medium, wherein the strain obtained by culture and screening of the high-concentration shenqinmycin culture medium is adopted as a starting strain for screening of the next round, and repeatedly carrying out the process for multiple rounds, thus obtaining the target strain. Compared with the prior art, the synergic disease-resistant bacterium has the advantages that the synergic disease-resistant bacterium has good drug resistance to shenqinmycin, and forms a mixed preparation with shenqinmycin, the use is safe, the prevention and treatment effects for rice sheath blight disease and watermelon fusarium wilt can be improved, the yield is increased, meanwhile, the use amount of shenqinmycin is reduced, so that the cost is reduced.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a synergistic anti-disease bacterium and its cultivation method and application. Background technique [0002] The control of fungal diseases such as rice, vegetables, and fruits is currently dominated by chemical pesticides, which have brought many adverse effects on the environment and the human body. Shenzimycin is a new type of biopesticide with high efficiency and low toxicity. High, resulting in a relatively small area of ​​use, how to improve the control of Shenzimycin on rice sheath blight and other fungal diseases, reduce the cost of use, and develop new preparations so that it can be more widely used in crop production Among them, it has become the research direction of technicians. [0003] At present, some bacillus are sold as a kind of biological pesticides in the market, but it is still relatively difficult to achieve the desired effect, and it cannot be applied together wi...

AI Technical Summary

Problems solved by technology

[0002] The control of fungal diseases such as rice, vegetables, and fruits is currently dominated by chemical pesticides, which have brought many adverse effects on the environment and the human body. Shenzimycin is a new type of biopesticide with high efficiency and low toxicity. High, resulting in a relatively small area of ​​use, how to improve the control of Shenzimycin on rice sheath blight and other fungal diseases, reduce the cost of use, and develop new preparations so that it can be more widely used in crop production It has become the research direction of technicians

[0003] At present, some bacillus are sold as a kind of biological pesticides on the market, but it is still difficult to achieve the desired effect, and it cannot be applied together with some antibacterial pesticides

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