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Preparation method of surface-carboxylation gold magnetic core shell nano-composite particles

A composite nanoparticle and nanoparticle technology, applied in the field of biomedical engineering, can solve problems such as no functional groups, and achieve the effects of good biocompatibility, dispersion stability, good biological functionality and multiple responsiveness

Inactive Publication Date: 2018-02-02
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the Fe prepared by these methods 3 o 4 @Au composite nanoparticles have no reactive functional groups on the surface. If they want to further compound with other materials, the surface must be further modified

Method used

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  • Preparation method of surface-carboxylation gold magnetic core shell nano-composite particles
  • Preparation method of surface-carboxylation gold magnetic core shell nano-composite particles
  • Preparation method of surface-carboxylation gold magnetic core shell nano-composite particles

Examples

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Effect test

Embodiment 1

[0025] A method for preparing surface carboxylated gold magnetic core-shell composite nanoparticles, comprising the steps of:

[0026] (1) Fe 3 o 4 Preparation of nanoparticles: 1.156g FeCl 3 ·6H 2O, 3.3012g of ammonium acetate, 0.3424g of trisodium citrate were dissolved in 60mL of ethylene glycol, and kept for 1 hour when heated to 170°C; after cooling to room temperature, the solution was transferred to the reactor and reacted at 200°C for 8 hours; After the end, perform magnetic separation, wash with absolute ethanol and water alternately for 3 times, and finally disperse in absolute ethanol.

[0027] (2) Fe 3 o 4 Surface modification of nanoparticles: ① Put 1.96g of maleic anhydride (maleic anhydride) in a 10mL round-bottomed flask, add 3.4mL of APTES dropwise under the condition of ice-water bath, put it into the rotor and stir, and wait until the reactants become into a white solid (APTES-COOH) to stop the reaction; ② Take 30mg Fe 3 o 4 Add the absolute ethanol ...

Embodiment 2

[0033] A method for preparing surface carboxylated gold magnetic core-shell composite nanoparticles, comprising the steps of:

[0034] (1) Fe 3 o 4 Preparation of nanoparticles: 1.156g FeCl 3 ·6H 2 O, 3.3012g of ammonium acetate, 0.3424g of trisodium citrate were dissolved in 60mL of ethylene glycol, and kept for 1 hour when heated to 170°C; after cooling to room temperature, the solution was transferred to the reactor and reacted at 200°C for 16 hours; After the end, perform magnetic separation, wash 4 times with absolute ethanol and water, and finally disperse in absolute ethanol.

[0035] (2) Fe 3 o 4 Surface modification of nanoparticles: ① same as Example 1; ② take 30mg Fe 3 o 4 Add the absolute ethanol solution of nanoparticles and 1mL ammonia water to 100mL absolute ethanol, disperse evenly and place in a water bath at 40°C for reaction; add 45μL TEOS and react for 2h; add 30mg APTES-COOH and react for another 7h to end the reaction. Magnetic separation or centr...

Embodiment 3

[0041] A method for preparing surface carboxylated gold magnetic core-shell composite nanoparticles, comprising the steps of:

[0042] (1) Fe 3 o 4 Preparation of nanoparticles: 1.156g FeCl 3 ·6H 2 O, 3.3012g of ammonium acetate, 0.3424g of trisodium citrate were dissolved in 60mL of ethylene glycol, and kept for 1 hour when heated to 170°C; after cooling to room temperature, the solution was transferred to a reaction kettle and reacted at 200°C for 10 hours; After the end, perform magnetic separation, wash with absolute ethanol and water alternately for 3 times, and finally disperse in absolute ethanol.

[0043] (2) Fe 3 o 4 Surface modification of nanoparticles: ① same as Example 1; ② take 30mg Fe 3 o 4 Add the absolute ethanol solution of nanoparticles and 2mL ammonia water to 100mL absolute ethanol, disperse evenly and place in a water bath at 40°C for reaction; add 30μL TEOS and react for 2h; add 60mg APTES-COOH and react for another 7h to end the reaction. Magnet...

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Abstract

The invention discloses a preparation method of surface-carboxylation gold magnetic core shell nano-composite particles, and belongs to the field of biomedical engineering. According to the preparation method, magnetic Fe<3>O<4> nano particles are prepared by means of a solvothermal method at first, surface modification is conducted on the magnetic Fe<3>O<4> nano particles by means of tetraethyl orthosilicate and silane coupling agent with carboxyl modified, the magnetic Fe<3>O<4> nano particles subjected to surface modification are subjected to surface sulfhydrylization with bovine serum albumin (BSA), then gold nano particles prepared with a sodium citrate reduction method are added, Fe<3>O<4> @ Au core shell nano-composite particles are obtained through formation and assembly of disulfide bonds, finally the nano-composite particles are subjected to surface carboxylation by means of 3-mercaptopropionic acid (MPA), and the surface-carboxylation gold magnetic core shell nano-compositeparticles are obtained. The surface-carboxylation gold magnetic core shell nano-composite particles are good in dispersity, high in stability and high in magnetic performance, the average hydration particle size is 860.2 nm, the distribution coefficient PDI is 0.103, the average Zeta potential is -30.1 mv, meanwhile the result of infrared spectroscopy indicates that the surfaces of the nano-composite particles contain carboxyl groups which can be further functionalized, and the application potential of the nano-composite particles in the biomedical field of targeted drug controlled release, thermal therapy, cell and protein separation and the like is expanded.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a method for preparing surface carboxylated gold magnetic core-shell composite nanoparticles. Background technique [0002] Magnetic nanoparticles such as Fe 3 o 4 Magnetic nanoparticles have unique magnetic properties and good biocompatibility, and show potential application prospects in biomedical fields such as separation of cells and biomolecules, magnetic resonance imaging, targeted drug delivery, hyperthermia, and stem cell labeling. Gold nanoparticles have excellent electrical and optical properties and good biocompatibility, and are easy to stably combine with biomacromolecules (such as proteins, nucleic acids, peptides), and are widely used in molecular recognition and biomarkers. In addition, gold nanoparticles have high light-to-heat conversion efficiency. After intravenous injection, they can be enriched in the tumor site. After being irradiated wit...

Claims

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Application Information

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IPC IPC(8): H01F1/00H01F1/11B01J20/22B01J20/28B01J20/32
CPCH01F1/0054B01J20/06B01J20/22B01J20/28009B01J20/3291H01F1/112
Inventor 郭惠玲孙宏浩刘明星祝红达孙红梅
Owner HUBEI UNIV OF TECH
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