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Dopamine detection method based on metal organic framework materials

A technology of metal-organic frameworks and detection methods, which is applied in the fields of material analysis, material excitation analysis, and material analysis through optical means. It can solve the problems of dopamine selective detection interference, etc., and achieve the effect of easy-to-operate selective detection and simple methods

Inactive Publication Date: 2020-01-07
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, the coexistence of dopamine (DA), ascorbic acid (AA), folic acid (FA) and uric acid (UA) in biological fluids interferes with the selective detection of dopamine

Method used

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  • Dopamine detection method based on metal organic framework materials
  • Dopamine detection method based on metal organic framework materials
  • Dopamine detection method based on metal organic framework materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Synthesis and determination of iodine-Cd(II) complexes based on 4-(1H-1,2,4-triazol-1-ylmethyl)aniline

[0036]Based on the iodine-Cd(II) complex of 4-(1H-1,2,4-triazol-1-ylmethyl)aniline, the general chemical formula is as follows:

[0037] [Cd(L) 2 I 2 ] (1); L is 4-(1H-1,2,4-triazol-1-ylmethyl)aniline, as a ligand, the structural formula is

[0038]

[0039] (1) with CdI 2 As a raw material, 4-(1H-1,2,4-triazol-1-ylmethyl)aniline is used as ligand L; 0.1831g (0.5mmol) CdI2 is weighed and dissolved in 5mL of water (i.e. uniformly dispersed), Weigh 0.1741g (1mmol) of ligand L and dissolve it in 5mL of methanol (that is, uniform dispersion), mix the above two solutions (that is, uniform dispersion), and then put it in a 15mL hydrothermal kettle and keep it at 160°C for three days (every day 24 hours), slowly cooling down (that is, natural cooling to room temperature 20-25 degrees Celsius) to obtain colorless transparent crystals.

[0040] (2) Accordin...

Embodiment 2

[0049] Embodiment 2: Prepare multiple ready-to-use solutions for subsequent implementation

[0050] (1) 100 mg L -1 Preparation of metal-organic framework material suspension: Weigh 0.0100g metal-organic framework material and dissolve it in 100mL high-purity water, use an ultrasonic cleaner to ultrasonically disperse for 5 minutes to form a uniform suspension, and store it in a dark place for later use.

[0051] (2) Preparation of 0.1M 25°C Tris-HCl buffer solution: Weigh 0.6058g of Tris (Tris) and dissolve it in 40mL of high-purity water, add hydrochloric acid to adjust the pH to 7.4, then add high-purity water to adjust the volume to 50mL, Store in the refrigerator until ready to use.

[0052] (3) Preparation of 10mmol / L potassium permanganate solution: Accurately weigh 0.0079g of potassium permanganate reagent and dissolve it in 5ml of high-purity water, shake well to dissolve it completely, and prepare a series of standard working solutions with different concentrations ...

Embodiment 3

[0054] Example 3: Determination of UV Spectrum and Fluorescence Emission Patterns of Metal Organic Framework Complex Materials Used in the Present Invention

[0055] Use a pipette gun to draw 600 μL metal-organic framework complex suspension (100 mg L -1 ), so that the metal-organic framework complex material concentration in the final system is 15mg L -1 1. Add 400 μL of Tris-HCl buffer solution (0.1M), then add high-purity water to make the volume to 4mL, and use a UV-visible spectrophotometer to detect the absorbance of the system; use a fluorescence spectrophotometer at an excitation wavelength of 242nm, an excitation slit of The fluorescence intensity of the detection system was detected under the conditions of 5nm slit and 660V photomultiplier tube voltage. as attached image 3 As shown, 1 is an ultraviolet spectrum diagram, and 2 is a fluorescence emission diagram. The metal-organic framework complex material used in the present invention has a strong absorption at 24...

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Abstract

The invention discloses a dopamine detection method based on a metal organic skeleton material. An iodine-Cd(II) complex based on 4-(1H-1,2,4-triazole-1-ylmethyl)aniline is adopted as the metal organic skeleton material and used as a fluorescent probe to selectively detect the content of dopamine. The method is simple and convenient, no complicated functionalization process is needed, and the selective detection for the dopamine can be realized.

Description

technical field [0001] The invention belongs to the field of preparation of metal-organic framework complexes and fluorescence sensing, and in particular relates to the application of a metal-organic framework complex in the selective detection of dopamine in a fluorescent "off-on" mode without labeling. Background technique [0002] Dopamine (DA) is an important neurotransmitter present in the central nervous system of mammals. It plays an important role in the nervous system, cardiovascular system, and regulation of kidney function and hormone secretion. When its content is abnormal, it can lead to some clinical diseases, such as Parkinson's disease, schizophrenia, Alzheimer's disease, depression and so on. Therefore, the detection of dopamine has become a topic of concern to researchers all over the world. In recent years, with the progress and development of materials science, the use of synthetic materials to analyze and detect biological small molecules has sprung up ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 李妍程月郭超丁斌王优优
Owner TIANJIN NORMAL UNIVERSITY