Application of 3 beta-acetoxyl group-7,25-kanziol diene-24R-alcohol to comprehensive prevention and treatment of lipid abnormity and diabetes
A technology of acetoxy and quincediene, which is applied in the field of medicine, can solve the problems that have not been seen, the glucose tolerance and insulin resistance of diabetic patients have little effect, and the blood lipid level cannot be significantly adjusted.
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Embodiment 1
[0022] Example 1: The lipid-lowering effect of 3β-acetoxy-7,25-calendadien-24R-ol
[0023] 1. Experimental animals and methods
[0024] C57BL mice, SPF grade, male, body weight (20±2) g, were randomly divided into two groups, the first group of 14 was the normal control group, given normal feed, and the remaining mice (34) were used to produce high-fat mice. The models were given high-fat feed (20% lard, 1.25% cholesterol, and 0.5% sodium cholate were added to the basic feed), and they were free to eat and drink. After 8 weeks of continuous feeding, 4 mice were sacrificed from the normal control group and the model mice respectively, and the serum and liver biochemical indicators of the two groups of mice, as well as the pathological sections of liver tissue, were compared to determine whether the model was established successfully. 30 mice successfully modeled were randomly divided into 3 groups, the model group (10 mice), the 3β-acetoxy-7,25-calendadiene-24R-alcohol group (...
Embodiment 2
[0037] Example 2: Hypoglycemic Effect of 3β-Acetoxy-7,25-Calendadien-24R-ol
[0038] 1. Experimental animals and methods
[0039] The modeling and administration methods are the same as those in Example 1, and the drug is administered by gavage continuously for 6 weeks after the modeling.
[0040] Glucose tolerance test method: After 6 weeks of continuous administration, the mice were fasted overnight, and after oral administration of glucose 2g / kg at 0, 15, 30, 60, 90, and 120 minutes respectively, the blood glucose was measured at the tip of the tail, and calculated using GraphPad Prism software The corresponding area under the line (AUC).
[0041] Insulin tolerance test method: After continuous administration of mice for 6 weeks, fasting overnight, intraperitoneal injection of insulin 0.75IU / kg at 0, 30, 60, 90, 120, and 150min, the tail tip blood was taken to measure the corresponding blood sugar, using GraphPad Prism software calculates the corresponding area under the ...
Embodiment 3
[0059] Embodiment 3: the application in pharmaceutical preparation or food
[0060] 1. 3β-Acetoxy-7,25-Granadien-24R-ol Tablets
[0061] 3β-Acetoxy-7,25-calendadien-24R-ol 10mg, starch 88g, magnesium stearate 3g.
[0062] Preparation process: Take 3β-acetoxy-7,25-camadien-24R-alcohol, mix with starch and magnesium stearate evenly, make granules, dry, and compress into tablets.
[0063] 2. 3β-Acetoxy-7,25-Candrene-24R-ol Capsules
[0064] 3β-Acetoxy-7,25-calendadien-24R-ol 10mg, starch 88g, magnesium stearate 3g.
[0065] Preparation process: Take 3β-acetoxy-7,25-camadien-24R-alcohol, mix with starch and magnesium stearate evenly, make granules, dry, pack into capsules, and obtain.
[0066] 3. 3β-Acetoxy-7,25-Mansadien-24R-ol Injection
[0067] 3β-Acetoxy-7,25-calendadien-24R-ol 47mg, sodium chloride for injection 7mg.
[0068] Preparation process: Dissolve 3β-acetoxy-7,25-camadien-24R-alcohol in 1.9 mL of water for injection, add sodium chloride for injection to isotonici...
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