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Method for detecting thrombin by virtue of SERS enhanced controllable chain-shaped assembly

A thrombin and assembly technology, which is used in measurement devices, instruments, material analysis by optical means, etc., can solve the problem that the stability of antibodies is easily affected by the environment, the preparation cycle of antibodies is long, and the sensitivity and specificity of test results are affected. problem, to achieve the effect of strong Raman signal enhancement, quantitative detection, and the effect of strong Raman enhancement

Active Publication Date: 2018-02-23
嘉兴劲境电子商务有限公司
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Problems solved by technology

[0005] Technical problem to be solved: The widely used immunological detection method for protein detection relies on the use of antibodies, and the preparation cycle of antibodies is long, and the stability of antibodies is easily affected by the environment, which further affects the sensitivity and specificity of detection results

Method used

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  • Method for detecting thrombin by virtue of SERS enhanced controllable chain-shaped assembly
  • Method for detecting thrombin by virtue of SERS enhanced controllable chain-shaped assembly

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Embodiment 1

[0029] A method for detecting thrombin with SERS-enhanced controllable chain assembly includes the following steps:

[0030] (1) Synthesis of Au@Ag nanoparticles and Ag nanoparticles

[0031] Add 96 mL of ultrapure water to a clean three-necked bottle, then add 2.5 chloroauric acid with a mass concentration of 0.4%, magnetically stir and heat to boil, add 1.5 mL of trisodium citrate with a mass concentration of 1% after 10 minutes, the solution is from After the colorless turns to wine red, the heating is stopped, and the stirring is continued for 15 minutes to obtain 20 nm gold nanoparticles. Take more than 5mL of synthesized gold nanoparticles and add 200μL of PVP solution with a mass concentration of 1%, 100μL of ascorbic acid solution with a concentration of 0.1M, and then add 70μL of silver nitrate solution with a concentration of 0.1mM, mix well, and shake at room temperature for 1h. The 50nm Au@Ag nanoparticles with 20nm Au core and 15nm thickness Ag shell are obtained.

[0...

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Abstract

The invention relates to a method for detecting thrombin by virtue of an SERS enhanced controllable chain-shaped assembly, belonging to the field of nano-sensing detection. According to the method, DNA molecules of two aptamers of the thrombin are respectively modified to the surfaces of an Au@ nano-particle and an Ag nano-particle, an alternate-arrayed chain-shaped structure is formed between thetwo nano-particles by controlling the DNA modification amounts of the surfaces of the nano-particles through the combination between assembly molecules and the thrombin when the thrombin exists, anda relatively strong Raman signal enhancement effect is achieved; and when the content of the thrombin is higher, the chain structure is longer, and the Raman signal strength is stronger, and the quantitative determination of the thrombin can be realized according to the corresponding relation between the content of the thrombin and the Raman signal strength. The method is simple in operation and high in sensitivity, and the thrombin in a serum sample can be detected; and by utilizing the nano-structure assembly, an important theoretical foundation is provided for detection of other target objects taking the aptamers as recognition molecules.

Description

Technical field [0001] The invention belongs to the field of nano-sensing detection, and in particular relates to a method for detecting thrombin with a SERS-enhanced controllable chain assembly. Background technique [0002] Protein is the main carrier of life activities and the main performer of biological functions. Almost all physiological and pathological processes can cause corresponding changes in protein. Therefore, protein detection is used in basic research in life sciences, human health evaluation, disease diagnosis, medicine and food Analysis and biosafety monitoring have high application value, and the detection of disease-related proteins is a very important task in the field of biomedical diagnosis. [0003] At present, the commonly used methods for protein detection are immunoassay methods based on the specific recognition of antigens and antibodies, mainly including Western Blot, ELISA, affinity chromatography, and protein chip, but this method requires It relies ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨蕾
Owner 嘉兴劲境电子商务有限公司
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