Method for preparing rare ginsenoside by using schizophyllum commune for biotransformation of ginsenoside

A technology of rare ginseng saponins and ginsenosides, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as insecurity, and achieve the effect of improving health care and medicinal efficacy

Active Publication Date: 2018-03-02
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It overcomes the shortcomings of using unsafe bacteria and molds that cannot...

Method used

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  • Method for preparing rare ginsenoside by using schizophyllum commune for biotransformation of ginsenoside
  • Method for preparing rare ginsenoside by using schizophyllum commune for biotransformation of ginsenoside
  • Method for preparing rare ginsenoside by using schizophyllum commune for biotransformation of ginsenoside

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Schizophyllum was inoculated into the liquid fermentation medium at an inoculum of 5%, the pH of the medium was 8, and ginsenoside Rb 1 The feeding amount was 5g / L, the rotation speed of the shaker was 120 r / min, and the fermentation was carried out at 20°C for 4 days. After the fermentation, the bacterial liquid was filtered, and the mycelium was ultrasonically extracted 3 times with 80% ethanol, 30 min each time. Extract the solvent under reduced pressure at 40°C, concentrate it into an aqueous solution, and then combine the concentrated extract with the bacterial solution; separate with macroporous resin D101, elute with a gradient of 20%-90% ethanol, and detect by thin-layer chromatography , collect and merge the fraction solutions with the same Rf value as the positive reference substance of ginseng rare saponins, recover the solvent under reduced pressure, separate and prepare by preparative liquid chromatography, and obtain ginsenoside F 2 and compound K. The c...

Embodiment 2

[0048] Schizophyllum was inoculated into the liquid fermentation medium at an inoculation amount of 10%, the pH of the medium was 7, and ginsenoside Rb 2 The feeding amount was 10g / L, the rotation speed of the shaker was 140 r / min, and the fermentation was carried out at 22°C for 5 days. After the fermentation, the bacterial liquid was filtered, and the mycelium was ultrasonically extracted 4 times with 80% ethanol, 30 min each time. The solvent was recovered from the extract under reduced pressure at 50°C, and concentrated into an aqueous solution. Then the concentrated extract was combined with the bacterial solution, separated with macroporous resin D101, 20%-90% ethanol gradient elution, detected by thin layer chromatography, collected and combined with the same Rf value of the positive reference substance of ginseng rare saponins The fraction solution was recovered under reduced pressure, and separated and prepared by preparative liquid chromatography to obtain compound ...

Embodiment 3

[0050] Schizophyllum was inoculated into the liquid fermentation medium with a 15% inoculation amount, the pH of the medium was 6, the feeding amount of ginsenoside Rc was 20g / L, the rotation speed of the shaker was 160 r / min, and the fermentation was carried out at 25°C. Cultured for 6 days. After the fermentation, the bacterial liquid was filtered, and the mycelium was ultrasonically extracted 5 times with 80% ethanol, 30 min each time. The solvent was recovered from the extract under reduced pressure at 60°C, and concentrated into an aqueous solution. Then the concentrated extract was combined with the bacterial solution, separated with macroporous resin D101, 20%-90% ethanol gradient elution, detected by thin layer chromatography, collected and combined with the same Rf value of the positive reference substance of ginseng rare saponins The fraction solution was recovered under reduced pressure and the solvent was separated and prepared by preparative liquid chromatography...

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Abstract

The invention discloses a method for preparing rare ginsenoside by using schizophyllum commune for biotransformation of ginsenoside. A novel method is used for biotransformation of ginsenoside, duringa fermentation process, schizophyllum commune is capable of degrading ginsenoside to generate a lot of rare saponins, a lot of various biologically-active substances such as schizophyllan, polypeptide, alkaloid, enzyme, amino acid, and vitamin can be simultaneously generated; and health care and medicinal efficacy of a fermentation product can be increased with a maximum. For biotransformation ofginsenoside, during the fermentation process, schizophyllum commune can degrade ginsenoside to generate a lot of rare saponins, a lot of various biologically-active substances such as schizophyllan,polypeptide, alkaloid, enzyme, amino acid, and vitamin can be simultaneously generated; and health care and medicinal efficacy of a fermentation product can be increased with a maximum. The method canbe used for preparing a monomer rare saponin, and can be directly extract a crude extract product for product exploitation, a simple processing purpose with high efficiency, safety, environment protection, and low cost can be achieved, and the method provides guarantee for industrial production, and food and new drugs preparation.

Description

technical field [0001] The invention relates to a preparation method of ginseng rare saponins, discloses a method for preparing ginseng rare saponins by using Schizophyllum biotransformation ginsenosides, and belongs to the technical field of liquid fermentation of edible and medicinal fungi. Background technique [0002] Ginsenosides are the main active ingredients of Panax ginseng, American ginseng and Panax notoginseng, which have the main physiological activities of Panax genus plants. So far, more than 100 kinds of ginsenosides have been isolated and their structures determined. Ginsenosides are divided into diol type ginsenosides (Rb 1 , Rc, Rb 2 , Rd, Rg 3 、Rh 2 , compound K, etc.) and triol type ginsenosides (Re, Rg 1 , Rg 2 , Rf, Rh 1 etc.), wherein ginsenoside Rb 1 , Rc, Rb 2 , Rd, Re and Rg 1 It is the main saponin of Panax genus, accounting for about 90% of ginsenosides. Rare ginsenosides, such as ginsenoside Rg 3 , F2, compound K, Rh 2 、Rh 3 etc.,...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12R1/645
CPCC12P33/20
Inventor 刘志李玉孙光芝阮长春王立娟
Owner JILIN AGRICULTURAL UNIV
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