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Nuclear-shell-shaped carbon nanohorn/lipidosome nano carrier and preparation method thereof

A technology of carbon nanohorns and nanocarriers, which is applied in the field of core-shell carbon nanohorns/liposome nanocarriers and its preparation, can solve the problems of poor prognosis of TAM, and achieve the ability to obviously control drug release and biocompatibility Good, high drug loading capacity effect

Inactive Publication Date: 2018-03-06
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, in many but not all human tumors, infiltration of a large number of TAMs is associated with poor prognosis

Method used

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  • Nuclear-shell-shaped carbon nanohorn/lipidosome nano carrier and preparation method thereof
  • Nuclear-shell-shaped carbon nanohorn/lipidosome nano carrier and preparation method thereof
  • Nuclear-shell-shaped carbon nanohorn/lipidosome nano carrier and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation method of core-shell nanocarriers

[0041] (1) SWCNHs with a length of 50-100 nm were added to a weak acid aqueous solution (30% H 2 O 2 ), ultrasonically dispersed for 30 min, condensed and refluxed for 4 h under mechanical stirring in an oil bath at 80 °C, cooled and allowed to stand for a period of time after the reaction, removed the upper layer of acid and diluted with distilled water, filtered through a 0.22 μm mixed cellulose filter membrane under vacuum, and the filter cake was The filtrate was washed with a large amount of deionized water until the pH value of the filtrate was neutral, and vacuum-dried at 50 °C to obtain O-SWCNHs;

[0042](2) Disperse O-SWCNHs in a methanol solution of doxorubicin (DOX), the mass ratio of doxorubicin and O-SWCNHs is 1:4; the mixture solution is sonicated for 30 min, and then phosphate with pH 7.4 is added dropwise Buffer solution (PBS), then use a cell crusher ultrasonic power 400W, ultrasonic for 2min, ...

Embodiment 2

[0046] Example 2 In vitro release determination of nanocarriers

[0047] The DOX-O-SWCNHs and pure drug DOX prepared in step (2) of Example 1 were dispersed in PBS solutions of pH 7.4 and pH 5.0, respectively, so that the DOX concentrations in the above four samples were kept at 70 μg / mL. Take 2mL of the sample solution and place it in a dialysis bag with a molecular weight cut-off of 8kDa, clamp the dialysis bag with a dialysis clip and put it into the corresponding 40mL PBS buffer solution, and place it in a horizontal constant temperature oscillator at 37 ° C for dynamic dialysis ( The frequency is 100 r / min), and the timing is started. After a certain period of time, 0.5 mL of dialysate is taken, and 0.5 mL of fresh PBS solution is supplemented at the same time. After centrifuging the taken samples, the content of DOX was determined by UV.

[0048] attached figure 2 In order to study the release curve of the drug in the functionalized SWCNHs (DOX-O-SWCNHs) drug-loading ...

Embodiment 3

[0049] Example 3 Cytotoxicity test of nanocarriers

[0050] (1) Cell culture: Non-small cell lung cancer A549 and normal cell 297T cells were selected, cultured in DMEM medium containing 10% fetal bovine serum (FBS), and placed at 37°C, 5% CO 2 cultured in an incubator.

[0051] (2) Detection of tumor cell survival rate: A549 and 293T cells in logarithmic growth phase were taken and 8 × 10 cells per well were used. 4 The cell density of cells / mL was inoculated in a 96-well culture plate, and the raw material SWCNHs and the carbon nanohorn drug delivery complex O-SWCNHs prepared in Example 1 were added to A549 and 293T cells in the logarithmic growth phase. %CO 2 Incubate for 24h and 48h under conditions. When the culture was stopped, wash twice with PBS, add 10 μL of WST-1 to each well, shake on a micro-shaker for 10 min, measure the absorbance values ​​at 450 nm and 630 nm with an enzyme-linked immunosorbent assay, and calculate the survival rate of tumor cells. . The ex...

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Abstract

The invention relates to a nuclear-shell-shaped carbon nanohorn / lipidosome nano carrier. The grain size of the carrier is 100 to 200nm. The carrier is prepared from pH sensitivity type lipidosome andcarbon oxide nanohorn wrapped by the lipidosome, wherein a polarization factor or polarization medicine of tumor related macrophages are loaded to the surface of the pH sensitivity type lipidosome, and an antitumor medicine is loaded to the carbon oxide nanohorn. The invention further provides a preparation method of the carrier. The preparation method comprises the steps: (1) evenly mixing the carbon oxide nanohorn and the antitumor medicine in an organic solvent, adding a buffer solution with a pH as 7.0 to 7.5 and evenly mixing to obtain a medicine loaded carbon nanohorn; (2) dissolving thepH sensitivity type lipidosome into the organic solvent, then adding water to hydrate, adding the polarization factor or the polarization medicine of the tumor related macrophages, evenly mixing andpenetrating through a membrane to be extruded out to obtain polarization factor or polarization medicine loaded pH sensitivity type lipidosome; (3) evenly mixing products of the step (1) and the step(2) and penetrating through the membrane to be extruded out to obtain the nuclear-shell-shaped carbon nanohorn / lipidosome nano carrier.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a core-shell carbon nanohorn / liposome nanocarrier and a preparation method thereof. Background technique [0002] Malignant tumor is one of the common diseases that seriously threaten human life and health in today's world. At present, the main treatment methods include surgery, chemotherapy and radiotherapy. Chemotherapy is a common treatment method for tumors. Since most anti-tumor drugs lack specificity to tumor cells, chemotherapy kills tumor cells and also kills normal cells, with large toxic and side effects. After chemotherapy drugs enter the systemic blood circulation through injection, they go through steps such as protein binding, metabolism, and excretion, and fewer drugs reach the tumor site. The combination of chemotherapy and immunotherapy allows the drug to reach the tumor site more efficiently and inhibit the growth of tumor cells. [0003] Macrophage classification ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/04A61K38/20A61K31/675A61K31/663A61K31/704A61P35/00A61P35/04
CPCA61K9/1271A61K31/663A61K31/675A61K31/704A61K38/20A61K47/02A61K2300/00
Inventor 曹青日张晓雪
Owner SUZHOU UNIV
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