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Calcium carbonate based compound micro particles and preparation and application thereof

A calcium carbonate and compound technology, which is applied to calcium carbonate-based compound particles and their preparation and application fields, can solve problems such as toxic and side effects, and achieve the effects of uniform particle size, content reduction, and reversal of tumor immunosuppressive microenvironment

Active Publication Date: 2020-11-06
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, radiotherapy and chemotherapy have obvious toxic and side effects, and can easily lead to drug resistance in tumors. Therefore, the development of a safe and efficient radiofrequency ablation combined treatment strategy is of great importance for further broadening the scope of application of existing radiofrequency ablation techniques in the clinical treatment of liver cancer. the meaning of

Method used

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  • Calcium carbonate based compound micro particles and preparation and application thereof
  • Calcium carbonate based compound micro particles and preparation and application thereof
  • Calcium carbonate based compound micro particles and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Preparation of polylactic acid-glycolic acid calcium carbonate particles

[0067] After weighing PLGA and PLGA-PEG according to the mass ratio of 1:1, they were dissolved in dichloromethane to prepare a 30 mg / ml mixed solution of PLGA and PLGA-PEG. Weigh NaHCO 3 with CaCl 2 Dissolved in water to prepare 0.625M NaHCO 3 Aqueous solution with 1.25M CaCl 2 aqueous solution. The prepared NaHCO 3 Aqueous solution with CaCl 2 The aqueous solution was mixed with PLGA and PLGA-PEG mixed solution according to the volume ratio of 1:3, and sonicated for 297 s with an ultrasonic cell disruptor under the condition of ultrasonic power of 100 W to obtain emulsion A (NaHCO 3 @PLGA-PEG) and Emulsion B (CaCl 2 @PLGA-PEG). The emulsion A and the emulsion B were mixed, and the emulsion C was obtained by ultrasonic emulsification with an ultrasonic cell disruptor for 297 s under the condition of an ultrasonic power of 100 W. The obtained emulsion C was dispersed in a 1% P...

Embodiment 2

[0068] Example 2: Property detection of polylactic acid-glycolic acid calcium carbonate particles

[0069] The polylactic acid-glycolic acid calcium carbonate particles prepared in Example 1 were qualitatively detected, and were respectively detected by transmission electron microscopy, dynamic light scattering and proton neutralization ability.

[0070] Among them, the results of transmission electron microscopy are as follows: figure 1 As shown in a, the results show that the polylactic acid-glycolic acid calcium carbonate particles prepared in Example 1 are distributed in a monodispersed state in water, indicating that the polylactic acid-glycolic acid calcium carbonate particles prepared by the present invention have a uniform particle size, and in water Good dispersion.

[0071] The results of dynamic light scattering detection are as follows figure 1 b, the results show that the polylactic acid-glycolic acid calcium carbonate particles prepared in Example 1 are in differ...

Embodiment 3

[0073] Example 3: Behavior detection of polylactic acid-glycolic acid calcium carbonate particles in mice

[0074] The behavior of the polylactic acid-glycolic acid calcium carbonate particles prepared in Example 1 in mice was detected. First of all, blood circulation detection was performed, and the fluorescent dye DIR-labeled polylactic-glycolic acid calcium carbonate microparticles were intravenously injected into mice, and the blood of mice was collected at different time points and the fluorescence intensity in the blood was detected. The result of blood circulation such as figure 2 As shown in a, the results show that the polylactic acid-glycolic acid calcium carbonate microparticles prepared in Example 1 have a longer blood circulation time in mice after intravenous injection. Tumor enrichment results such as figure 2 b shows, figure 2 In b, the dashed circle represents the fluorescence of the tumor site. Fluorescence imaging results of tumor-bearing mice proved ...

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Abstract

The invention relates to calcium carbonate based compound micro particles and preparation and application thereof. The calcium carbonate based compound micro particles disclosed by the invention comprise calcium carbonate and a macromolecule stable layer, wherein the macromolecule stable layer comprises amphipathic molecules and / or hydrophilic molecules. The invention further discloses applicationof the calcium carbonate based compound micro particles to preparation of one or more of an adjuvant, a chemotherapy enhancing adjuvant, a radiotherapy enhancing adjuvant and a radiofrequency ablation treatment adjuvant for tumor immnuotherapy. The calcium carbonate based compound micro particles disclosed by the invention have an excellent effect of regulating a tumor slight acid environment, can reduce content of immunosuppressive cells in tumor, increase content of immune cells capable of killing the tumor and promote polarization of tumor-associated macrophages from the M2 type to the M1type, effectively turns the tumor immunosuppression micro environment, activate antitumor immunity, and can enhance a suppression effect of various treatment means of immunotherapy, chemotherapy, radiotherapy, radiofrequency ablation treatment and the like on tumor growth.

Description

technical field [0001] The invention relates to the field of tumor treatment preparations, in particular to a calcium carbonate-based composite particle and its preparation and application. Background technique [0002] Tumor immunotherapy is a treatment method that inhibits tumor growth by activating the body's own immune system. Its core is to effectively activate the anti-tumor response of T lymphocytes in tumor patients to achieve specific inhibition and killing of tumor growth. After years of development, cancer immunotherapy can be mainly divided into four aspects: immune modulators, immune checkpoint blockade therapy, adoptive immunotherapy and immune vaccines. [0003] Although activating the body's immune function to destroy cancer cells has broad prospects, traditional immunotherapy also exposes many toxic side effects associated with excessive immunity during treatment, such as low response to immune checkpoint inhibitors, which are only effective for some patient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K31/4188A61K31/704A61K39/39A61K41/00A61K45/00A61K47/02A61K47/34A61P35/00
CPCA61K9/167A61K9/1611A61K9/1647A61K33/10A61K39/39A61K41/00A61K45/00A61K31/704A61K31/4188A61P35/00A61K2039/55505A61K2039/55555A61K2300/00
Inventor 刘庄冯良珠杨志娟郝钰朱宇杰
Owner SUZHOU UNIV
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