6-dansylamide-indole fluorescent probe and its preparation method and application
A technology of dansylamide and fluorescent probes, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of differentiation, difficulty, difficult culture medium, etc., and achieve accurate detection results, low cost, and easy operation simple effect
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Embodiment 1
[0034] In this embodiment, the name and structural formula of the 6-dansylamide-indole fluorescent probe are as follows:
[0035]
[0036] The preparation method of above 6-dansylamide-N-methyl-indole is as follows:
[0037] Weigh dansyl chloride (1327 mg, 4.92 mmol) dissolved in 3 ml of pyridine, weigh 6-amino-indole (500 mg, 3.78 mmol) dissolved in 4 ml of pyridine, slowly drop into the dansyl chloride solution, Stirred under ice bath for 2 hours to room temperature, protected by argon, reacted overnight. 100 ml of double distilled water was added to the reaction to terminate the reaction, and the reaction mixture was precipitated. The precipitated solid was dissolved with 100 ml of ethyl acetate, washed twice with 60 ml of sodium bicarbonate, twice with 20 ml of saturated sodium chloride, and twice with 50 ml of water. Sodium sulfate was added to remove water, and 1954 mg of intermediate was obtained through column chromatography;
[0038]Intermediate 1 (60 mg, 0.164 ...
Embodiment 2
[0042] In this embodiment, the name and structural formula of the 6-dansylamide-indole fluorescent probe are as follows:
[0043]
[0044] The above 6-dansylamide- N -Ethyl-indole is prepared as follows:
[0045] Weigh dansyl chloride (1327 mg, 4.92 mmol) dissolved in 3 ml of pyridine, weigh 6-amino-indole (500 mg, 3.78 mmol) dissolved in 4 ml of pyridine, slowly drop into the dansyl chloride solution, Stirred under ice bath for 2 hours to room temperature, protected by argon, reacted overnight. 100 ml of double distilled water was added to the reaction to terminate the reaction, and the reaction mixture was precipitated. The precipitated solid was dissolved with 100 ml of ethyl acetate, washed twice with 60 ml of sodium bicarbonate, twice with 20 ml of saturated sodium chloride, and twice with 50 ml of water. Sodium sulfate was added to remove water, and 1954 mg of intermediate was obtained through column chromatography;
[0046] Intermediate 1 (60 mg, 0.164 mmol) and ...
Embodiment 3
[0050] In this embodiment, the name and structural formula of the 6-dansylamide-indole fluorescent probe are as follows:
[0051]
[0052] The above 6-dansylamide- N -Propyl-indole is prepared as follows:
[0053] Weigh dansyl chloride (1327 mg, 4.92 mmol) dissolved in 3 ml of pyridine, weigh 6-amino-indole (500 mg, 3.78 mmol) dissolved in 4 ml of pyridine, slowly drop into the dansyl chloride solution, Stirred under ice bath for 2 hours to room temperature, protected by argon, reacted overnight. 100 ml of double distilled water was added to the reaction to terminate the reaction, and the reaction mixture was precipitated. The precipitated solid was dissolved with 100 ml of ethyl acetate, washed twice with 60 ml of sodium bicarbonate, twice with 20 ml of saturated sodium chloride, and twice with 50 ml of water. Sodium sulfate was added to remove water, and 1954 mg of intermediate was obtained through column chromatography;
[0054] Intermediate 1 (60 mg, 0.164 mmol) and...
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