Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antarcticibacterium flavum JB01H24 and application thereof

An aerobic denitrification and bacteria technology, applied in the field of microorganisms and environmental science, can solve the problems such as aerobic denitrification bacteria that have not been found

Inactive Publication Date: 2018-03-13
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No aerobic denitrifying bacteria have been found in Flavobacteriaceae

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antarcticibacterium flavum JB01H24 and application thereof
  • Antarcticibacterium flavum JB01H24 and application thereof
  • Antarcticibacterium flavum JB01H24 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation of aerobic denitrifying bacteria (Antarcticibacterium flavum) JB01H24

[0021] The marine sediment samples were collected from the Antarctic Ross Sea (165°34'9.84"E, 77°35'17.88"S) at a water depth of 774m. 1g of sediment sample (wet weight) was added to 10mL of sterile seawater, fully shaken and mixed, and the suspension dilution was applied to the Marine Agar 2216 (MA, BDDifco) plate by the standard dilution coating method, and placed in 4 Cultivate at ℃ for 1 month; then pick the colonies from the plate and streak and purify, the transmission electron microscope photos of the obtained strain JB01H24 are as follows figure 1 shown.

[0022] The purified strain JB01H24 was stored in a glycerol tube (-80°C) and a lyophilized tube (4°C) respectively. Experiments have found that the strain JB01H24 is generally cultured with Marine Agar or Marine Broth 2216 (MB, BDDifco) at a culture temperature of 25-28°C.

Embodiment 2

[0023] Example 2 Identification of aerobic denitrifying bacteria (Antarcticibacterium flavum) JB01H24

[0024] The genome of strain JB01H24 obtained in Example 1 was extracted by CTAB method: its 16S rRNA gene was amplified using 27F and 1492R primers, and connected to the cloning vector PCR2.1. The length of the 16SrRNA sequence obtained after sequencing is 1482bp, and the nucleotide sequence is shown in SEQ ID NO:1. Using MEGA 7.0.25, Kimura 2-parameter model, NJ and ML algorithm to build a tree (bootstrap repeated 1000 times), the final result of the evolutionary tree is as follows figure 2 . Identification result: the bacterial strain JB01H24 that embodiment 1 isolates is a new genus, named Antarcticibacterium, is the type species of this genus of bacteria; The full name of the strain is named: aerobic denitrifying bacteria (Antarcticibacterium flavum) JB01H24.

[0025] In the EzBioCloud database, the 16S rRNA gene sequence of the aerobic denitrifying bacteria (Antarct...

Embodiment 3

[0047] Example 3 Study on denitrification efficiency of aerobic denitrifying bacteria (Antarcticibacterium flavum) JB01H24

[0048] Insert the aerobic denitrifying bacteria (Antarcticibacteriumflavum) JB01H24 into the denitrification medium according to the inoculum size of 1%, culture at 28°C and 180rpm for 5 days, and respectively at 0h, 4h, 8h, 12h, 16h, 24h, 32h, 40h, 48h , 60h, 72h, 96h, 120h, take samples, measure the culture OD, and detect NO 3 - (thymol colorimetric method), the result is as follows image 3 .

[0049] Wherein, the denitrification medium includes the following components: 2g KNO 3 , 2.5g sodium succinate, 2.5g sodium citrate, 1gK 2 HPO 4 , 1g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 330mL seawater, add double distilled water to 1L, adjust pH to 7.4, sterilize with moist heat, add 1 / 50 compound carbon source, 1 / 500 compound vitamin solution, 1 / 1000 compound trace element mixed solution before use.

[0050] The preparation method of the trace element m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Antarcticibacterium flavum JB01H24 and an application thereof. The Antarcticibacterium flavum JB01H24 belongs to the Flavobacteriaceae in the Bacteroidetes according to the taxonomy and is preserved in the Guangdong microbiological culture collection center (GDMCC) in floor 5th, building 59th, courtyard 100th, Xianlie middle road, Guangzhou city, Guangdong province on August 31, 2017. After the Antarcticibacterium flavum JB01H24 is cultured in a denitrification medium with an initial concentration of nitrate nitrogen of 14 mg / L under 180 rpm and 28 DEG C for 72h, the nitrate nitrogen removal rate is greater than 95% and thus the Antarcticibacterium flavum JB01H24 can be used for treating nitrogen oxides and has a great significance for the development of a denitrification technology.

Description

technical field [0001] The invention belongs to the field of microorganism and environmental science and technology, and more specifically, the invention relates to a novel aerobic denitrification bacterium (Antarcticibacterium flavum) JB01H24 and its application in treating nitrogen oxides. Background technique [0002] With the development of industry and agriculture and the intensification of human activities, a large amount of nitrogen enters the water body through various channels, resulting in increasingly serious nitrogen pollution in the water body, endangering the safety of the ecosystem, the environment and human health. Therefore, the denitrification of water body has become the focus and focus of water treatment research. [0003] At present, in the field of environmental engineering and sewage / wastewater treatment, microbial denitrification technology has become the most widely used denitrification technology, with low cost, high efficiency, easy operation, no s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/163C02F2101/166C12N1/205C12R2001/01
Inventor 李安章张明霞姚雨欣邹卫玲朱红惠姚青
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com