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A method for detecting fingertip blood wetness method and its kit and device

A detection reagent and wet method technology, which is applied in the field of fingertip blood wet method detection, can solve the problems of test result deviation, blood cell rupture, sample size difficult to separate, etc., and achieve the effect of simple composition and accuracy

Active Publication Date: 2021-05-14
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fingertip blood is whole blood, and the sample size is too small to separate
Moreover, fingertip blood is directly tested by wet method, and there will be two problems: one is that during the reaction process, blood cells rupture, releasing hemoglobin and other components to interfere with the test; Volume of whole blood, under different blood pressure volumes, the amount of serum is different, and the final test results will have large deviations
Therefore, fingertip blood can hardly be used in current wet assays.

Method used

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  • A method for detecting fingertip blood wetness method and its kit and device
  • A method for detecting fingertip blood wetness method and its kit and device
  • A method for detecting fingertip blood wetness method and its kit and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: A device for fingertip blood wetness detection

[0048] Such as figure 1 and 2 , the device for fingertip blood wetness detection includes a fingertip blood sample dilution coagulation area 3, a hemoglobin detection area 4 and a target analyte detection area 5, the fingertip blood sample dilution coagulation area 3, a hemoglobin detection area 4 Connected with the target analyte detection area 5, the fingertip blood suction device 2 and the pipetting device 1 are fixed on the fingertip blood sample dilution and coagulation area to form an integral device.

Embodiment 2

[0049] Embodiment 2, the kit that is used for fingertip blood wet method detection:

[0050] It includes the device for detecting fingertip blood wetness described in Example 1, an agglutination reagent for red blood cells, a detection reagent for hemoglobin and a detection reagent for a target analyte.

Embodiment 3

[0051] Embodiment 3, the detection of triglyceride:

[0052] Adopt the kit described in embodiment 2 to detect,

[0053] In the detection device, the fingertip blood sample dilution and coagulation area 3, the hemoglobin detection area 4 and the target analyte detection area 5 contain corresponding reagents, and the solution formula of each area is as follows:

[0054] Fingertip blood sample dilution and coagulation area 3: the erythrocyte agglutination reagent is physiological saline containing anti-erythrocyte antibody;

[0055] Hemoglobin detection area 4: the detection reagent of hemoglobin is made up of potassium ferricyanide, 50Mm, pH7.0 dipotassium hydrogen phosphate, 0.1% triton;

[0056] Target analyte detection area 5: Triglyceride detection reagent consists of piperazine-1,4-diethanesulfonic acid buffer (pipis buffer) (50mM, pH6.8), LPL (2200U / L), GK (250U / L), POD (1000U / L), magnesium chloride (40mM), sodium cholate (3.5mM), ATP (1.5μM), 4-aminoantipyrine (1mM), ...

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Abstract

The invention belongs to the field of biochemical detection, and in particular relates to a method for fingertip blood wetness detection, a kit and a device thereof. In the detection method of the present invention, fingertip blood wetness detection is carried out by using the method of the present invention, and the red blood cells in the fingertip blood sample are deposited by the red blood cell agglutination reagent in the first solution, so that the blood cells coagulate rapidly, thereby achieving serum and red blood cell Separation to avoid interference with target analytes due to blood cell rupture. The fingertip blood sample diluted by the first solution is subjected to a second reaction with the second solution containing the hemoglobin detection reagent, the hemoglobin concentration is detected by a colorimetric method, and the hematocrit is obtained by calculation. Calculate the correction value of the target analyte concentration according to the obtained hematocrit result, avoid the problem of deviation in the final test result caused by different hematocrit, and improve the accuracy of the test result. The device and kit for detecting fingertip blood wetness described in the present invention are simple in composition, and are widely applicable to the detection of various targets.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and in particular relates to a blood detection method, in particular to a method for fingertip blood wetness detection, a kit and a device thereof. Background technique [0002] In vitro diagnosis, namely IVD (In Vitro Diagnosis), refers to products and services that obtain clinical diagnostic information by testing human samples (blood, body fluids, tissues, etc.) outside the human body, and then determine diseases or body functions. [0003] In vitro diagnostic testing methods can basically be divided into wet method and dry method. The reaction system of wet method is liquid phase, which is the most widely used method. For example, most of the current biochemical analyzers use wet method for testing. The reaction system of the dry method is solid, such as dry chemical test paper. In the current test and analysis, most of the analytes are analytes in serum, so blood cells and serum need to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N15/05
CPCG01N15/05G01N21/78
Inventor 李宗祥
Owner SINOCARE
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