Method for producing soybean isolate protein by high-temperature acid precipitation method

A technology of soybean protein isolate and acid precipitation, which is applied in the field of extraction of soybean protein isolate, can solve the problems of wasting protein resources, protein loss, and increasing the difficulty of sewage treatment, so as to reduce protein content, reduce COD value, and reduce the difficulty of sewage treatment. Effect

Inactive Publication Date: 2018-03-20
LINYI YUWANG VEGETABLE PROTEIN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, within this range, part of the protein cannot be precipitated, resulting in protein loss. The lost protein needs to be precipitated by polyalu

Method used

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  • Method for producing soybean isolate protein by high-temperature acid precipitation method

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Embodiment 1

[0051] A method for producing soybean protein isolate by high-temperature acid precipitation, comprising the steps of:

[0052] a) Extraction: mix the raw material low-temperature defatted soybean meal with water at a ratio of 1:8 by weight, add sodium hydroxide to adjust the pH value to 6.8, water temperature 45°C, stir for 15 minutes, use a multi-stage homogeneous pump to pulverize and transport to the storage tank , use microwave sterilization for 5 minutes, store for 30 minutes and wait for separation;

[0053] b) centrifugal separation: centrifuging the extract in step a) to obtain primary bean dregs and primary soymilk, washing the primary bean dregs with water to obtain secondary bean dregs and secondary soymilk, and mixing the primary soymilk and secondary soymilk;

[0054] c) acid precipitation: adjust the pH value of the mixture of primary soybean milk and secondary soybean milk to 4.3, and carry out sedimentation; add 0.2‰ Tween 80 with solid content before sedimentat...

Embodiment 2

[0062] A method for producing soybean protein isolate by high-temperature acid precipitation, comprising the steps of:

[0063] a) Extraction: Mix the raw material low-temperature defatted soybean meal with water at a ratio of 1:10 by weight, add sodium hydroxide to adjust the pH value to 7.5, water temperature 35°C, stir for 15 minutes, use a multi-stage homogeneous pump to pulverize and transport to the storage tank , use microwave sterilization for 2 minutes, store for 30 minutes and wait for separation;

[0064] b) centrifugal separation: centrifuging the extract in step a) to obtain primary bean dregs and primary soymilk, washing the primary bean dregs with water to obtain secondary bean dregs and secondary soymilk, and mixing the primary soymilk and secondary soymilk;

[0065] c) Acid precipitation: adjust the pH value of the mixture of primary soybean milk and secondary soybean milk to 4.8, and carry out sedimentation; add 0.3‰ Tween 80 with solid content before sedimen...

Embodiment 3

[0073] A method for producing soybean protein isolate by high-temperature acid precipitation, comprising the steps of:

[0074] a) Extraction: mix the raw material low-temperature defatted soybean meal with water in a ratio of 1:5 by weight, add sodium hydroxide to adjust the pH value to 7.0, water temperature 40°C, stir for 10 minutes, use a multi-stage homogeneous pump to pulverize and transport to the storage tank , use microwave sterilization for 3 minutes, store for 20 minutes and wait for separation;

[0075] b) centrifugal separation: centrifuging the extract in step a) to obtain primary bean dregs and primary soymilk, washing the primary bean dregs with water to obtain secondary bean dregs and secondary soymilk, and mixing the primary soymilk and secondary soymilk;

[0076] c) Acid precipitation: adjust the pH value of the mixture of primary soybean milk and secondary soybean milk to 4.5, and carry out sedimentation; add 0.1‰ Tween 80 with solid content before sediment...

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Abstract

The invention discloses a method for producing soybean isolate protein by a high-temperature acid precipitation method. The method comprises the following steps: (1) mixing low-temperature defatted soybean meal as a raw material and water with the temperature of 35-45 DEG C according to a weight ratio of 1:(5 to 15), regulating a pH value to 6.8-7.5, crushing and sterilizing after stirring, and separating to obtain primary soybean residue and primary soybean milk; (2) regulating the pH value of the soybean milk obtained in the step (1) to 4.3-4.8, and carrying out sedimentation; (3) heating protein suspension after sedimentation, then carrying out flash evaporation for degassing, cooling, and separating to obtain acid solid-phase protein slurry; (4) adding water into the acid solid-phase protein slurry, mixing and then regulating the pH to 6.5-7.5; (5) carrying out sterilization, flash evaporation for degassing, drying and recovery on neutralized feed liquid obtained in the step (4) toobtain a soybean isolate protein product. The method for producing the soybean isolate protein by the high-temperature acid precipitation method, disclosed by the invention, has the beneficial effects that the content of protein in whey waste water can be reduced, the acid-precipitation loss of the protein can be reduced, the recovery rate of the soybean protein can be increased, the treating difficulty of the whey waste water can be reduced, the use amount of polymeric aluminium chloride can be reduced, and the environmental pollution and the danger control can be reduced.

Description

technical field [0001] The invention relates to the technical field of extracting soybean protein isolate, in particular to a method for producing soybean protein isolate by high-temperature acid precipitation. Background technique [0002] Soybean protein is currently the only reported cholesterol-free plant protein that contains 9 essential amino acids needed by the human body and meets the needs of the human body. It is recognized as a complete protein that can supplement the animal protein and grain protein diets Defects. However, the soy protein intake of Chinese residents is relatively low, especially the intake of soy protein is only 5%, which is far below the recommended amount of 20%. On the basis of affirming the nutritional function of soybean protein, researchers have never given up on the research on the health function of soybean protein for a long time. A large number of experiments have proved that soybean protein has the functions of lowering blood fat and ...

Claims

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Application Information

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IPC IPC(8): C07K1/30C07K1/14
CPCC07K1/14C07K1/30
Inventor 马军时玉强刘汝萃崔玉涛
Owner LINYI YUWANG VEGETABLE PROTEIN
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