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Method for building biological activity gel film layer on surface of biological material containing alkynyl

A technology of biological activity and biomaterials, applied in the field of tissue engineering, can solve problems such as weak binding force, poor modification effect, desorption, etc., and achieve the effect of mild reaction conditions, wide application range and strong flexibility

Inactive Publication Date: 2018-03-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complexity of the structure of the porous scaffold material, the surface of the internal material of the scaffold can only be modified by physical adsorption. The modified molecules bind to the surface through van der Waals force, the binding force is weak, and it is easy to desorb from the surface. Difference
Lack of a method to modify the surface of the material inside the scaffold, so that the modified molecules and the surface can be chemically bonded together

Method used

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  • Method for building biological activity gel film layer on surface of biological material containing alkynyl
  • Method for building biological activity gel film layer on surface of biological material containing alkynyl
  • Method for building biological activity gel film layer on surface of biological material containing alkynyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) forming a film of polyurethane (Mn=70000) containing alkyne groups on glass, with a mass of about 0.2 g, to obtain a polyurethane film containing alkyne groups;

[0038] (2) Add 0.2 g of alkyne-containing polyurethane film (the content of the alkyne group is about 1 mmol / g) (2eq) and 0.5 g of PEG2000 modified with azide groups (the content of the azide group is 0.9 mmol / g) (2eq) into 10ml of water, deoxygenated by nitrogen for 30min; weigh 0.002gCuBr / 0.002gPMDETA (0.01eq / 0.1eq) and dissolve in 0.3ml of anaerobic water to obtain an aqueous solution of the catalyst; add the aqueous solution of the catalyst to the reaction system, under nitrogen protection React at 30°C for 24 hours, take out the polyurethane film from the reaction solution, wash with methanol / water (V / V=1:1) to remove copper ions and unreacted small molecules, dry in vacuum, and compound the PEG gel film layer on the surface of the polyurethane material , to obtain a polyurethane film bonded with a PE...

Embodiment 2

[0049] (1) Electrospinning PAA (Karebay Biochem, Inc, with an alkynyl content of about 1 mmol / g) (Mn=65800) containing alkyne groups (hexafluoroisopropanol as a solvent, and a concentration of 6% in the spinning solution) , the voltage is 12kV, the injection speed is 1ml / h, the pole distance is 10cm), the average pore size is 400μm, and the mass is 1g, and the PAA three-dimensional scaffold containing alkyne groups is obtained;

[0050] (2) 1g of PAA three-dimensional scaffold containing alkyne group (alkyne group content is about 1mmol / g) (1eq) and 1g chitosan modified with azido group (azido group content 2mmol / g) (2eq) were added to In 30ml of water, pass nitrogen gas to remove oxygen for 40min, add copper sulfate pentahydrate 0.008g (0.2eq) and sodium ascorbate 0.03(1eq), react at 37°C for 10h under nitrogen protection, take the three-dimensional scaffold out of the reaction solution, EDTA / ethanol (V / V=1:1) washing to remove copper ions and unreacted small molecules, vacu...

Embodiment 3

[0056] (1) Three-dimensional printing (T=205°C P=3.5bar) of polyurethane containing alkyne groups (Mn=35000), with an average pore size of 400 μm and a mass of 5 g, to obtain a three-dimensional polyurethane scaffold containing alkyne groups;

[0057] (2) Add 5 g of polyurethane three-dimensional scaffolds containing alkyne groups (alkyne group content 1 mmol / g) (1 eq) and 4 g of gelatin modified with azido groups (azido groups about 2 mmol / g) (1.6 eq) into 50 ml of water , deoxygenated with nitrogen for 30 minutes, added 0.003g of copper powder, reacted at 50°C for 15 hours under the protection of nitrogen, took the three-dimensional scaffold out of the reaction solution, washed with EDTA / ethanol (V / V=1:1) to remove copper powder and unreacted Small molecules, vacuum-dried, gelatin gel layer compounded on the surface of the polyurethane scaffold material to obtain a polyurethane scaffold bonded with gelatin;

[0058] (3) Add the gelatin-bonded polyurethane scaffold and 4 g of...

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Abstract

The invention belongs to the technical field of tissue engineering, and discloses a method for building a biological activity gel film layer on the surface of a biological material containing alkynyl.The method comprises the following steps that (1) performing reaction on a high molecular material containing alkynyl and biological active macromolecules containing azide groups to obtain a high molecular material with biological active macromolecules; (2) performing reaction on the biological active macromolecules containing alkynyl and a high molecular material bonded with biological active macromolecules to obtain a high molecular material bonded with an active layer; (3) repeating the step (1) or the steps (1) to (2) to obtain the high molecular materials bonded with active layers with different thickness on the surface; (4) performing reaction on micromolecules containing azide groups or alkynyl and the high molecular materials in the step (3) to obtain a product. The method provided by the invention is simple, flexible and efficient; the reaction conditions are mild; the thickness of the biological active layer can be controlled; no limitation exists on the material form morphological structure; the application range is wide; the combination of the biological active layer and the high molecular materials is stable and firm.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a method for constructing a biologically active gel film layer on the surface of an alkyne-containing biological material. Background technique [0002] As one of the three major elements of tissue engineering, tissue engineering scaffold materials provide a three-dimensional environment for tissue engineering cells to grow. Biocompatibility is also extremely important. On the one hand, it is necessary to avoid triggering inflammatory or teratogenic reactions. On the other hand, as a biological carrier of cells, it is necessary to endow the material with certain biological functions, such as facilitating cell adhesion and providing good support for tissue repair. environment etc. At present, many polymer materials used in tissue engineering scaffolds lack biological activity and cannot play a good role in repairing, replacing or treating. Since a single m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/50A61L27/52A61L27/54
CPCA61L27/34A61L27/50A61L27/52A61L27/54A61L2300/252A61L2300/606A61L2400/18A61L2420/02A61L2420/08C08L71/02C08L5/08C08L89/06
Inventor 吴刚朱美潼方立明刘章拴王舒浩
Owner SOUTH CHINA UNIV OF TECH
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