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Novel use of acetate-containing small molecules as peroxidases acting as catalysts

A technology of peroxidase and small molecules, applied in the field of simulated enzymes, can solve the problems of limited sources, extensive development and use restrictions, heat sensitivity and poor stability of horseradish peroxidase, and achieve easy access and low price Inexpensive, good stability

Inactive Publication Date: 2018-03-23
GUANGDONG IND TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, horseradish peroxidase has poor sensitivity and stability to heat, and its sources are limited. These defects have greatly restricted its extensive development and use in biosensors.

Method used

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  • Novel use of acetate-containing small molecules as peroxidases acting as catalysts
  • Novel use of acetate-containing small molecules as peroxidases acting as catalysts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The mensuration of embodiment 1 peroxidase activity

[0033] Dissolve 0.82g of sodium acetate in 10mL of water, and add 0.1mL of the above solution into 0.9mL of buffer systems with different pHs (including 0.05mol / L Tris-HCl, 1mmol / L H 2 o 2 , 1mmol / L 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS)).

[0034] The result is as figure 1 As shown, the small molecule has a good catalytic effect under the conditions of pH 1.0, 2.0, 3.0, 4.0, 5.0, and 6.0. The darker the color, the better the catalytic effect.

Embodiment 2

[0035] Preparation and application of embodiment 2 hydrogen peroxide detection test paper

[0036] (1) Preparation of impregnation solution: Take a 1000mL beaker, add 40mL sodium alginate solution (concentration is 50g / L), add 10mL 1% (v / v) Tween 80 solution, add 40mL gelatin solution (concentration is 80g / L) L), add 100mL pH 5.0 acetic acid-sodium acetate buffer solution (100mM sodium acetate), 10mL potassium iodide (50g / L), 10g polyvinylpyrrolidone, 0.5g brilliant blue, and mix well to obtain an impregnation solution;

[0037] (2) Immersion paper: Pour the impregnation solution prepared in step (1) into a glass dish, soak the filter paper, scrape off the excess solution with a glass rod, and dry it in a blast oven at 40°C for 30 minutes. After drying, it is Light blue test paper, obtains described hydrogen peroxide detection test paper.

[0038] like figure 2 As shown, the different concentrations of hydrogen peroxide to be tested (0 μM, 10 μM, 20 μM, 50 μM, 100 μM, 1000 ...

Embodiment 3

[0039] Preparation and application of embodiment 3 hydrogen peroxide detection test paper

[0040] (1) Preparation of impregnation solution: Take a 1000mL beaker, add 40mL sodium alginate solution (concentration is 50g / L), add 10mL 1% (v / v) Tween 80 solution, add 40mL gelatin solution (concentration is 80g / L) L), add 100mL pH 5.0 acetic acid-potassium acetate buffer solution (100mM potassium acetate), 10mL potassium iodide (50g / L), 10g polyvinylpyrrolidone, 0.5g brilliant blue, and mix well to obtain an impregnation solution;

[0041] (2) Immersion paper: Pour the impregnation solution prepared in step (1) into a glass dish, soak the filter paper, scrape off the excess solution with a glass rod, and dry it in a blast oven at 40°C for 30 minutes. After drying, it is Light blue test paper, obtains described hydrogen peroxide detection test paper.

[0042] Different concentrations of hydrogen peroxide (0 μM, 10 μM, 20 μM, 50 μM, 100 μM, 1000 μM, 2000 μM) to be tested were droppe...

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Abstract

The invention discloses a novel use of acetate-containing small molecules as peroxidases acting as catalysts. It is disclosed herein for the first time that acetate-containing small molecules have peroxidase activity. The acetate-containing small molecules have clear molecular structure, are easy to obtain and insensitive to heat, have good stability and low price and are easy to commercially develop. The invention also successfully constructs a novel method of detecting hydrogen peroxide based on the novel use and successfully applies the novel method in test papers to detect hydrogen peroxide, glucose, uric acid, cholesterol and the like. The peroxidase activity based on the small molecules is applicable to the fields of food, environment and sanitation and the like, and the small molecules have a promising application prospect.

Description

technical field [0001] The invention belongs to the field of simulated enzymes, and in particular relates to the new use of acetate-containing small molecules as peroxidase-like catalysts. Background technique [0002] Horseradish peroxidase is a biocatalyst produced by long-term evolution in nature. It can efficiently and exclusively catalyze the chemical reaction of hydrogen peroxide under mild conditions. Designing horseradish peroxidase-based biosensors is a common strategy used by researchers today, that is, with the assistance of enzymes, the specific binding of antibodies and antigens outputs readable electrochemical and optical signals. This method is easy to miniaturize The signal can be read by the naked eye, which can satisfy people's daily monitoring of their physical condition. As an efficient catalyst, horseradish peroxidase is widely used in food hygiene, environmental protection, forensic and other fields. [0003] However, horseradish peroxidase has poor ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/75G01N21/78G01N33/52
CPCG01N21/75G01N21/78G01N33/52G01N2021/7759
Inventor 栗瑞敏邓毛程李静陈维新王瑶周爱芳
Owner GUANGDONG IND TECHN COLLEGE
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