Check patentability & draft patents in minutes with Patsnap Eureka AI!

Full premix rapid human HLA-B*27 gene detection kit and method thereof

A gene detection and HLA-B technology, applied in the field of biomedical clinical molecular detection, can solve the problems of cumbersome data analysis, high price, long cycle, etc., and achieve the effect of widespread promotion value, easy operation and low cost.

Inactive Publication Date: 2018-03-27
SUZHOU KUANGYUAN MOLECULAR BIOTECH
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the disadvantages of the prior art, such as high price, long period, low efficiency, cumbersome data analysis, etc. when detecting HLA-B*27 alleles, and provides a fully premixed rapid human HLA- B*27 gene detection kit and method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Full premix rapid human HLA-B*27 gene detection kit and method thereof
  • Full premix rapid human HLA-B*27 gene detection kit and method thereof
  • Full premix rapid human HLA-B*27 gene detection kit and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1. Design of primers and probes:

[0084] According to the reference sequence of the HLA-B gene (NG_023187) published by the NCBI nucleic acid sequence database GeneBank of the National Center for Biotechnology Information in the United States (NG_023187) and the information of HLA-B*27 (HLA00223) published by the British IMG / HLA database, Primer Express of ABI Company was used 3.0 software respectively designed primers and probes for detecting HLA-B*27 and other genes. Primers and probes, as follows:

[0085] Forward primer:

[0086] HLA-B*27F: 5’-GGCTACGTGGACGACACGCTGT-3’

[0087] Reverse primer:

[0088] HLA-B*27R: 5’-GCAGGCTCTCTCGGTCAGTCTGTGCC-3’

[0089] Fluorescent probes:

[0090] TM-HLA-B*27: 5’FAM-CGTGAGGTTCGACAGCGACGCCGCG-3’BHQ1

[0091] In order to monitor the effectiveness of the reaction system, internal control primers and probes are added to the detection system. The present invention selects a sequence of the human conserved gene GAPDH (its r...

Embodiment 2

[0097] Example 2. Preparation of primers

[0098] The designed primers and probe sequences are handed over to the synthesis company for synthesis, generally using automatic chemical synthesis with instruments, and a synthesis inspection report is required.

Embodiment 3

[0099] Example 3: Preparation of the whole premixed rapid human HLA-B*27 gene detection kit

[0100] Prepare a reaction solution for detecting human HLA-B*27 alleles, in which the PCR reaction solution contains not only buffers, magnesium ions, dNTPs and other substances necessary for PCR reactions, but also detection primers and probes, internal control primers and probes, and Antibody-modified TaqDNA polymerase and PCR protectant trehalose. The concentration of the PCR reaction solution components and the sequence information of the included primers and probes for the above-mentioned specific detection are as follows:

[0101] Table 1. Components of PCR reaction solution

[0102] Reaction solution components

concentration

buffer

10×

Magnesium ions

15mM

Dntp MIX

10mM

Upstream Detection Primers

2µM

Downstream Detection Primers

2µM

detection signal probe

2µM

upstream internal control primer

1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a fully premixed rapid human HLA-B*27 gene detection kit and method. According to the principle of allele-specific PCR, human leukocyte antigen HLA-B*27 alleles are detected on a real-time fluorescent quantitative PCR technology platform . In addition, modified Taq DNA polymerase and disaccharide PCR protective agent are added to the reaction system to increase the stability of the reaction system and realize the long-term stability of the pre-mixed detection system.

Description

technical field [0001] The invention belongs to the field of biomedical clinical molecular detection, and in particular relates to a kit and a detection method for detecting HLA gene HLA-B*27 alleles. Background technique [0002] HLA antigen is the expression product of human major histocompatibility complex (Major Histocompatibility Complex, MHC). In the immune system, it is mainly responsible for the mutual recognition between cells, the induction of immune response, and the regulation of the function of immune response. HLA antigens can be divided into three categories: Class I molecules are HLA-A, -B, and -C series antigens, which are widely distributed on the surface of nucleated cells in various tissues, including platelets and reticulocytes, and mature red blood cells generally do not contain HLA antigens; Class II molecules are HLA-D / DR, -DP, and DQ series antigens, which are mainly expressed on B cells and antigen-presenting cells. These two types of antigens are r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 王进吴梦华吴子侠彭飞
Owner SUZHOU KUANGYUAN MOLECULAR BIOTECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com