Method for measuring carbohydrates in water by using fluorescence spectrum

A technology of fluorescence spectroscopy and sugar substances, which is applied in fluorescence/phosphorescence, measuring device, material analysis by optical means, etc., which can solve the distinction of hydroxymethyl furfural, the inability of simultaneous quantitative analysis, and the different yields of hydroxymethyl furfural, etc. question

Active Publication Date: 2018-03-27
UNIV OF SCI & TECH OF CHINA
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Problems solved by technology

However, because glucose and fructose produce HMF in different mechanisms during the dehydration process, the HMF yields of these two sugars are different, and the anthrone method cannot distinguish the source of HMF. The measurement results of the method are limited by the proportion of sugar in the sample, and the content of the two cannot be quantitatively analyzed at the same time.
It can be seen that the anthrone method cannot quantitatively analyze the aldose and ketose in the sample at the same time, leaving room for the improvement of this technology

Method used

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  • Method for measuring carbohydrates in water by using fluorescence spectrum
  • Method for measuring carbohydrates in water by using fluorescence spectrum
  • Method for measuring carbohydrates in water by using fluorescence spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Simultaneous quantitative analysis of glucose and fructose by sulfuric acid-fluorescence spectrometry

[0076] 1.1 Prepare 1g / L glucose solution and fructose solution as sample stock solution.

[0077] 1.2 Dilute the stock solution step by step to 0-50 mg / L as the standard solution of glucose and fructose.

[0078] 1.3 Add 2 mL of sample to a clean test tube and add 2.5 mL of concentrated sulfuric acid (98% v / v) and mix well.

[0079] 1.4 Place the mixed sample in a boiling water bath for 10 minutes, then cool it down to room temperature in a cold water bath.

[0080] 1.5 Use LS55 fluorescence spectrometer (Perkin-Elmer, Inc, USA) to measure the fluorescence spectrum of the sample. The slit for excitation and emission light was set at 10 nm, and the scanning speed was 1200 nm / min. The emission spectrum ranges from 300nm to 550nm with a step size of 0.5nm; the excitation light ranges from 320nm to 450nm with a step size of 10nm. All samples were tested 3 ti...

Embodiment 2

[0086] Example 2 Quantitative analysis of polysaccharides by sulfuric acid-fluorescence spectroscopy

[0087] 2.1 Prepare 1g / L dextran, lactose, sucrose, sorbitol and gluconic acid solution as the sample stock solution.

[0088] 2.2 Dilute the stock solution of dextran, lactose and sucrose to 0-50 mg / L, and dilute sorbitol and gluconic acid to 0-200 mg / L.

[0089] 2.3 Add 2 mL of sample to a clean test tube and add 2 mL of concentrated sulfuric acid (98% v / v) and mix well.

[0090] 2.4 Place the mixed sample for 2 minutes and cool it down to room temperature in a cold water bath.

[0091] 2.5 Use LS55 fluorescence spectrometer (Perkin-Elmer, Inc, USA) to measure the fluorescence spectrum of the sample. Fluorescence intensities were measured at two excitation emission positions (excitation 320 nm, emission 425 nm and excitation 375 nm, emission 455 nm).

[0092] 2.6 Use linear regression to decompose the fluorescence data and calculate the concentration of glucose and fructo...

Embodiment 3

[0098] Embodiment 3 Sulfuric acid-fluorescence spectrometry is to the validity analysis of actual sample test

[0099] 3.1 Configure 1g / L glucose solution and fructose solution as sample stock solution.

[0100] 3.2 Dilute the stock solution stepwise to 0-50mg / L at intervals of 10mL as standard solutions of glucose and fructose.

[0101] 3.3 From the center of East Chaohu Lake (31°32.081′N, 117°38.845′E), the center of West Chaohu Lake (31°38.341′N, 117°21.416′E), the upper reaches of Nanfei River (31°50′12.05″ 5 natural sample. Glucose and fructose of 0-50 mg / L were added to all samples at intervals of 10 mg / L to obtain 36 spiked recovered samples for measurement.

[0102] 3.4 Add 2 mL of sample to a clean test tube and add 2.5 mL of concentrated sulfuric acid (98% v / v) and mix well.

[0103] 3.5 Place the mixed sample in a boiling water bath for 8 minutes, then cool to room temperature.

[0104] 3.6 Use a Horiba Aqualog fluorescence spectrometer (Horiba Co., Japan) to m...

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Abstract

The invention provides a method for measuring carbohydrates in water by using a fluorescence spectrum. The method comprises the following steps: adding concentrated sulfuric acid into a to-be-tested sample and heating to obtain a reacted to-be-tested sample; carrying out fluorescent spectroscopy detection on the reacted to-be-tested sample to obtain fluorescence intensity; obtaining the content ofthe ketose and the aldose in the to-be-tested sample according to a fluorescence intensity standard model of ketose and aldose, wherein the heating time is greater than or equal to 0 minute. Comparedwith the prior art, the method disclosed by the invention has the advantages that by adding the concentrated sulfuric acid into the to-be-tested sample, the carbohydrates which have no fluorescent signals are dehydrated to generate fluorescent hydroxymethyl furfural and other fluorescent by-products; by measuring the fluorescence signals of products, quantitative analysis of the carbohydrates inthe water can be realized; in addition, different fluorescence data can be identified by utilizing different ways of generating hydroxymethyl furfural by the aldose and the ketose and fluorescence differences of reaction by-products , thereby realizing quantitative analysis of the contents of the aldose and the ketose in the to-be-tested sample at the same time.

Description

technical field [0001] The invention belongs to the technical field of water treatment, in particular to a method for measuring carbohydrates in water by fluorescence spectroscopy. Background technique [0002] As a very basic biomolecule, carbohydrates participate in a variety of complex biochemical processes and play an important role in various engineering applications. Therefore, the quantitative detection of carbohydrates in water is very important. In order to characterize sugars in water, staining methods for microscopy, biological and physicochemical methods have been widely used, but the above methods cannot quantitatively analyze the content of total sugars in large-volume samples. [0003] At present, the technologies that can quantitatively analyze carbohydrates mainly include boric acid method and anthrone method. However, the boric acid method relies on the binding of boric acid or its derivatives to sugars, so some non-sugar substances that can bind to boric ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6417
Inventor 钱晨俞汉青宫博
Owner UNIV OF SCI & TECH OF CHINA
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