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Glycine-glucose-exopolysaccharide compound feed additive

A feed additive and exopolysaccharide technology, which is applied in animal feed, pharmaceutical formula, additional food elements, etc., to achieve the effect of improving sensitivity, improving immunity and body function

Active Publication Date: 2018-04-10
福建九为生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, glycine is mainly used as a nutritional additive and attractant for increasing amino acids in feed for livestock and poultry, especially pets. So far, there are no reports of glycine promoting antibiotics to inhibit the growth of drug-resistant bacteria and glycine and glucose combined to promote the action of antibiotics. The Report on Combination of Glycine, Glucose and Exopolysaccharide as Feed Additive

Method used

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  • Glycine-glucose-exopolysaccharide compound feed additive
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  • Glycine-glucose-exopolysaccharide compound feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047]Determination of drug resistance of Edwardsiella tarda EIB202

[0048] Edwardsiella tarda is a Gram-negative short bacillus. It was first reported by Hoshina (1 96 2a), and it is related to reddisease of Japanese eel. From the first report to now, the fungus has caused diseases in more than 20 kinds of fish, such as eel, flounder, tilapia, Chinese soft-shelled turtle, carp, etc., causing huge losses to aquaculture. Edwardsiella tarda is also a common pathogenic bacteria in humans and fish, which directly poses a threat to human health.

[0049] Firstly, the minimum inhibitory concentration of Edwardsiella tarda to various antibiotics was determined. The results showed that the minimum inhibitory concentration of Edwardsiella tarda EIB202 to kanamycin was 12.5 μg / mg, the minimum inhibitory concentration to tetracycline was 125 μg / mg, and the minimum inhibitory concentration to chloramphenicol was 50 μg / mg, indicating that Edwardsiella tarda EIB202 is a multidrug resist...

Embodiment 2

[0051] Glycine increases the susceptibility of Edwardsiella tarda to kanamycin

[0052] 1. Preparation of test samples: Pick a single colony of Edwardsiella tarda EIB202 from the LB plate and inoculate it in 5 mg of LB medium, culture at 200 rpm at 30°C for 24 hours to reach saturation. Collect the bacterial liquid by centrifugation, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD value of the bacterial solution to 0.2, and then pack them separately 5mL in a test tube for later use.

[0053] 2. Glycine can increase the sensitivity of Edwardsiella tarda EIB202 to kanamycin

[0054] In order to know whether glycine contributes to the bactericidal effect of bacteria, the experiment of adding glycine under the premise of adding kanamycin was carried out. To the above prepared bacterial samples, first add 40 μg / mL kanamycin, then...

Embodiment 3

[0056] Glucose synergizes with glycine to increase susceptibility of Edwardsiella tarda

[0057] It has been reported in the literature that glucose can increase the sensitivity of aminoglycosides to Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) persistent drug-resistant bacteria (persistence), and it has been proved that this phenomenon is the Unique to glycoside antibiotics. We therefore also investigated the susceptibility of bacteria to kanamycin for glucose, and glycine and glucose.

[0058] 1. Glucose and glycine can synergistically increase the susceptibility of Edwardsiella tarda to kanamycin

[0059] In order to study whether glucose and glucose can cooperate with glycine to increase the sensitivity of bacteria to kanamycin, the experiment was divided into three groups: one group only added 40 μg / mL kanamycin in the prepared samples as a control, and the other two groups On the basis of adding 40 μg / mL kanamycin, 10 mM glucose, 10 mM glu...

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Abstract

The invention discloses a glycine-glucose-exopolysaccharide compound feed additive and relates to feed additives. The feed additive comprises, by mass, 0.01% to 50.0% of glycine, 0.01% to 50.0% of glucose, 0.01% to 50.0% of exopolysaccharide and the balance of carriers. A mass ratio of the glycine-glucose-exopolysaccharide compound feed additive to feed is 0.05-5%. Through use of glycine, the survival rate of Edwardsiella tarda in kanamycin treatment is decreased significantly so that the glycine can improve the sensitivity to kanamycin. Through use of glycine and glucose, the survival rate ofEdwardsiella tarda in kanamycin treatment is decreased significantly. The glycine and glucose can produce synergism and have a gradient effect of a glycine concentration.

Description

technical field [0001] The invention relates to a feed additive, in particular to a compound feed additive of glycine glucose exopolysaccharide. Background technique [0002] Although the use of antibiotics plays an essential role in the protection of human health and life and intensive animal farming, due to the abuse and misuse of antibiotics, it has become a threat to human health, livestock and poultry farming, aquaculture and the ecological environment. The key factor. Therefore, the control of bacterial antibiotic resistance is very important. [0003] At present, antibiotics are widely used in animal husbandry. On the one hand, some antibiotics are essential as veterinary drugs to control bacterial infections; on the other hand, some antibiotics can promote animal growth as feed drug additives. The extensive use of antibiotics has caused a large number of sensitive bacteria to die, and drug-resistant bacteria to multiply, which has promoted and enhanced bacterial r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/142A23K20/163A23K50/30A23K50/60A23K50/80A61K31/715A61K31/198A61K31/7004A61P31/04A61P37/04
CPCA61K31/198A61K31/7004A61K31/715A23K20/142A23K20/163A23K50/30A23K50/60A23K50/80A61K2300/00Y02A40/818
Inventor 谢荔朋
Owner 福建九为生物技术有限公司