Method for extracting corn yellow pigment food additive through laser assistance

A technology of corn yellow pigment and food additives, which is applied in the food field, can solve the problems of high extraction conditions, narrow application range, complicated operation, etc., and achieve the effects of low equipment and raw material costs, accelerated extraction efficiency, and good safety

Inactive Publication Date: 2018-04-13
CHENDU NEW KELI CHEM SCI CO LTD
7 Cites 1 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0010] In view of the currently widely used corn yellow pigment solvent extraction method, the requirements for reagents and extraction conditions are high and the effect is poor. At the same time, the general high-tech auxiliary extraction method has the problems of long processing time, complicated operation, expensive equipment and high cost. , and the extraction ef...
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Abstract

The invention provides a method for extracting a corn yellow pigment food additive through laser assistance. The method comprises the following steps of compounding corn protein powder tiny powder into suspension liquid, performing enzymolysis with cellulase and neutral protease, adding precipitate obtained through filtering enzymatic hydrolysate into ethanol, assisting extraction with laser illumination, after an extraction solution is filtered, taking supernatant, removing protein through chitosan flocculation, performing decompressed concentration, and performing vacuum freeze drying so asto obtain the corn yellow pigment food additive. According to the method, enzymes in corn can be activated through laser illumination, cell walls are thoroughly destroyed, pigment is released to the maximum extent, the extraction efficiency is high, the yield is high, the safety is good, and the corn yellow pigment food additive can be used as a coloring agent and an additive of various foods. Besides, the cost of equipment and raw materials is low, and the method is suitable for industrialized production.

Application Domain

Food science

Technology Topic

Freeze dryNeutral protease +12

Image

  • Method for extracting corn yellow pigment food additive through laser assistance
  • Method for extracting corn yellow pigment food additive through laser assistance

Examples

  • Experimental program(6)
  • Comparison scheme(1)

Example Embodiment

[0036] Example 1
[0037](1) Grind corn gluten powder into superfine powder with a superfine pulverizer, stir and mix with water to prepare a suspension, adjust the pH value to 9.5, adjust the temperature to 53°C, add cellulase, and use biphenyl after 2 hours of enzymatic hydrolysis Three phenols are used to inactivate the enzyme, then the pH value is adjusted to 6.5, the temperature is adjusted to 52°C, neutral protease is added, and the enzymolysis is carried out for 2.5 hours; part, 80 parts of water; the particle size of corn gluten powder superfine powder is 0.5 μ m; the enzyme amount of cellulase is 1%; the enzyme amount of neutral protease is 1.2%;
[0038] (2) Centrifuge and filter the enzymatic solution, remove the supernatant, add ethanol to the resulting precipitate, mix well, and then use a laser to irradiate repeatedly, completely destroy the cell wall of the corn, and further activate the enzyme in the corn, The various pigment components are released to the greatest extent, and dissolved in ethanol, centrifuged and filtered after 30 minutes, and the supernatant is collected; in the ethanol extract, the total mass parts of each component are calculated in 100 parts Among them, 30 parts of enzymatic hydrolysis sediment and 70 parts of ethanol; the filter centrifuge is a three-legged centrifuge; the laser instrument is a horizontal laser instrument; the power of the laser is 20mW;
[0039] (3) Add chitosan to the supernatant of step (2), adjust the pH value to 5, the temperature is 40°C, stir at a speed of 40r/min for 3min, let it stand for 4h, a large amount of free chitosan molecules Under the action of the amino group, the protein molecule flocculates and precipitates. After filtration, the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain the corn yellow pigment food additive; the addition of the chitosan is 0.01% of the supernatant quality. %; The temperature of vacuum freeze-drying is -40℃, and the vacuum degree is 4Pa.
[0040] Example 1 The method for extracting zeaxanthin, the total extraction time, yield, food safety and extraction cost are shown in Table 2.

Example Embodiment

[0041] Example 2
[0042] (1) Use a superfine pulverizer to pulverize corn gluten powder into superfine powder, stir and mix with water to prepare a suspension, adjust the pH value to 9, adjust the temperature to 54°C, add cellulase, and use continuous Glucoglucinol is used to inactivate the enzyme, then the pH value is adjusted to 6.5, the temperature is adjusted to 52°C, neutral protease is added, and the enzymolysis is carried out for 3.5 hours; 15 parts, 85 parts of water; the particle size of superfine corn gluten powder is 0.8 μm; the enzyme amount of cellulase is 1.4%; the enzyme amount of neutral protease is 1.1%;
[0043] (2) Centrifuge and filter the enzymatic solution, remove the supernatant, add ethanol to the resulting precipitate, mix well, and then use a laser to irradiate repeatedly, completely destroy the cell wall of the corn, and further activate the enzyme in the corn, The various pigment components are released to the greatest extent, and dissolved in ethanol, centrifuged after 25min, and the supernatant is collected; in the ethanol extract, the total mass parts of each component are calculated in 100 parts, Among them, 28 parts of enzymatic hydrolysis sediment and 72 parts of ethanol; the filter centrifuge is an overhead centrifuge; the laser instrument is a vertical laser instrument; the power of the laser is 18mW;
[0044] (3) Add chitosan to the supernatant of step (2), adjust the pH value to 6.3, the temperature is 32°C, stir at a speed of 48r/min for 3min, let stand for 2h, a large amount of free chitosan molecules Under the action of the amino group, the protein molecule flocculates and precipitates. After filtration, the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain the corn yellow pigment food additive; the addition of the chitosan is 0.02% of the supernatant quality. %; The temperature of vacuum freeze-drying is -20℃, and the vacuum degree is 8Pa.
[0045] Example 2 The method for extracting zeaxanthin, the total extraction time, yield, food safety and extraction cost are shown in Table 2.

Example Embodiment

[0046] Example 3
[0047] (1) Grind corn gluten powder into superfine powder with a superfine pulverizer, stir and mix with water to prepare a suspension, adjust the pH value to 9.5, adjust the temperature to 53°C, add cellulase, and use continuous Glucoglucinol is used to inactivate the enzyme, then the pH value is adjusted to 7.5, the temperature is adjusted to 49°C, neutral protease is added, and the enzymolysis is carried out for 3 hours; parts, 88 parts of water; the particle size of superfine corn gluten powder is 0.6 μm; the enzyme amount of cellulase is 1.2%; the enzyme amount of neutral protease is 1.2%;
[0048] (2) Centrifuge and filter the enzymatic solution, remove the supernatant, add ethanol to the resulting precipitate, mix well, and then use a laser to irradiate repeatedly, completely destroy the cell wall of the corn, and further activate the enzyme in the corn, The various pigment components are released to the greatest extent, and dissolved in ethanol, centrifuged after 22min, and the supernatant is collected; in the ethanol extract, the total mass parts of each component are calculated in 100 parts, Among them, 28 parts of enzymatic hydrolysis sediment and 72 parts of ethanol; the filter centrifuge is a scraper unloading centrifuge; the laser instrument is a horizontal laser instrument; the power of the laser is 15mW;
[0049] (3) Add chitosan to the supernatant of step (2), adjust the pH value to 6, the temperature is 35°C, stir at a speed of 55r/min for 3min, let it stand for 3h, a large amount of free chitosan molecules Under the action of amino groups, protein molecules flocculate and precipitate, and after filtration, the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain corn yellow pigment food additives; the addition of the chitosan is 0.03% of the supernatant quality. %; The temperature of vacuum freeze-drying is -30℃, and the vacuum degree is 5Pa.
[0050] Example 3 The method for extracting zeaxanthin, the total extraction time, yield, food safety and extraction cost are shown in Table 2.

PUM

PropertyMeasurementUnit
Particle size0.5 ~ 1.0µm

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