Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of method for preparing antibacterial collagen based on safrole epoxidation modification

A safrole and epoxidation technology, which is applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems such as the lack of long-term effect of continuous dissolution antibacterial mode, affecting the biocompatibility of natural collagen, and achieve antibacterial effects. Functional long-lasting, mild response conditions

Active Publication Date: 2022-03-08
YIBIN UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, the continuous dissolution antibacterial mode must lack long-term effect
Secondly, while many traditional antibacterial agents effectively kill harmful microorganisms such as bacteria, they also show physiological toxicity to mammals, and their addition will affect the inherent biocompatibility of natural collagen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of method for preparing antibacterial collagen based on safrole epoxidation modification
  • A kind of method for preparing antibacterial collagen based on safrole epoxidation modification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Safrole epoxidation modification:

[0022] Dissolve 12 parts of m-chloroperoxybenzoic acid in 100 parts of dichloromethane, add 8 parts of safrole, and stir at 20°C for 24 hours; thereafter, the product is washed 3 times with 2% aqueous sodium hydroxide solution, and then used to remove Ionized water was washed 3 times; finally, the oil layer was dried with anhydrous magnesium sulfate, filtered and distilled under reduced pressure to obtain epoxidized safrole;

[0023] (2) Epoxidized safrole grafted modified collagen:

[0024] Dissolve 10 parts of collagen in 0.2mol / L acetic acid aqueous solution, then coat the above mixture to form a film, and dry it at 20°C to constant weight; then soak the above collagen film in pH value 7, epoxidized safrole concentration 3% aqueous solution; after 12 hours, the collagen film was taken out and washed three times with deionized water to obtain epoxidized safrole grafted modified collagen.

[0025] The results of infrared spectr...

Embodiment 2

[0027] (1) Safrole epoxidation modification:

[0028] Dissolve 15 parts of iodosobenzene in 150 parts of chloroform, add 10 parts of safrole, and stir for 20 hours at 25°C; after this, the product is washed 4 times with 5% potassium hydroxide aqueous solution, and then washed with deionized water for 5 time; at last, the oil layer is dried with anhydrous magnesium sulfate, and after filtration, vacuum distillation obtains epoxidized safrole;

[0029] (2) Epoxidized safrole grafted modified collagen:

[0030] Dissolve 15 parts of collagen in 0.4 mol / L acetic acid aqueous solution, then coat the above mixture to form a film, and dry it at 22°C to constant weight; then soak the above collagen film in pH 8, epoxidized safrole concentration 7% aqueous solution; after 24 hours, the collagen film was taken out and washed 4 times with deionized water to obtain epoxidized safrole grafted modified collagen.

[0031] The results of infrared spectroscopy and circular dichroism experimen...

Embodiment 3

[0033] (1) Safrole epoxidation modification:

[0034] Dissolve 20 parts of tert-butanol peroxide in 200 parts of carbon tetrachloride, add 12 parts of safrole, and stir at 30° C. for 12 hours; after this, the product is washed 5 times with 7% aqueous sodium bicarbonate solution, and then used to remove Washing with deionized water for 5 times; finally, the oil layer was dried with anhydrous magnesium sulfate, filtered and distilled under reduced pressure to obtain epoxidized safrole;

[0035] (2) Epoxidized safrole grafted modified collagen:

[0036] Dissolve 20 parts of collagen in 0.5 mol / L acetic acid aqueous solution, then coat the above mixture to form a film, and dry it at 25°C to constant weight; then soak the above collagen film in pH 9, epoxidized safrole concentration 10% aqueous solution; after 48 hours, the collagen film was taken out and washed 5 times with deionized water to obtain epoxidized safrole grafted modified collagen.

[0037] The results of infrared s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing antibacterial collagen based on safrole epoxidation modification. The method uses a weak epoxidation reagent to modify the safrole, and then uses the covalent reaction between the epoxy group and the amino group on the side chain of the collagen macromolecule to covalently introduce the safrole into the collagen, so as to endow the collagen substrate with good antibacterial activity. The collagen material obtained by this modification method has a long-term antibacterial function, the triple helical structure of the collagen substrate will not be destroyed, and its inherent biocompatibility can also be completely preserved. It is applied in the field of collagen-based biomedical materials bright future.

Description

technical field [0001] The invention relates to a method for preparing antibacterial collagen based on safrole epoxidation modification, which belongs to the field of biomass materials. Background technique [0002] Collagen is the most important structural protein in the connective tissue of higher vertebrates. It exists widely in animal tissues in the form of skin, bones, tendons, ligaments, nerves, blood vessels, and teeth, and plays a role in supporting organs and protecting biological organisms. Collagen is the most abundant protein in mammals, accounting for about 25-33% of the total protein in animals. The special triple-helix conformation of collagen endows it with many excellent biological properties, such as degradability, hemostasis, and tissue compatibility, so that it can be used in tissue engineering such as skin substitutes, artificial cartilage, and artificial blood vessels, as well as in various fields. It has been widely used in a variety of medicine and h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K1/113C07K1/02
CPCC07K14/78
Inventor 徐洲范浩军陈意常金明官小玉王忠辉曾琦
Owner YIBIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com