Preparation method of antibacterial preservative

A technology of bactericidal preservatives and mass ratio, applied in the direction of bactericides, botanical equipment and methods, biocides, etc., can solve the problems of easy pollution in the production and use process, difficult preparation of bactericidal preservatives, and difficult cleaning of oil emulsions, etc. Achieve the effects of eliminating hemolytic toxicity, strong adhesion, and improved antibacterial properties

Inactive Publication Date: 2018-05-18
CHANGZHOU DAAO NEW MATERIAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The technical problem to be solved by the present invention: the current bactericidal preservatives have poor water solubility, cannot be completely dissolved into the oil emulsion and are difficult to clean in silk processing, have high toxicity, have an impact on the human body and the production environment at the concentration used, and are acidic or The acidic chemical fiber oil bactericidal preservative is not easy to be prepared into a water-solvent transparent preparation, and it is easy to pollute in the process of production and use, the drug effect is low, the production cost is high, and the service life is short. A preparation method for the bactericidal preservative is provided.

Method used

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  • Preparation method of antibacterial preservative

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preparation example Construction

[0026] A preparation method of a bactericidal preservative, comprising the steps of:

[0027] (1) Take the transglutaminase powder and add it to the PBS buffer solution with a concentration of 0.2 mol / L at a mass ratio of 1:8, stir and mix at 30~35°C for 20~30min to obtain a stirred mixture, take the stirred mixture at 3000 r / L After centrifuging for 10 min, take the supernatant A for vacuum filtration, take the filtrate for dialysis, and freeze-dry to obtain dry powder A for future use;

[0028] (2) Take sulfur and add sodium methoxide and methanol at a mass ratio of 6:11:25~20, stir and mix at -3~-4°C for 1~2h, then add benzyl chloride with 50~60% methanol mass, and heat up to 75 Keep at ~80°C for 12~15h, cool down, filter with suction, take the filtrate a and distill it under reduced pressure, take the vacuum distillate a according to the mass ratio of 1:10:15, add deionized water and ether to mix, let it stand, and collect the supernatant B. Rotary evaporation to obtain r...

Embodiment 1

[0033] Transglutaminase powder: purchased from Wuhan Huashun Biotechnology Co., Ltd.

[0034] A preparation method of a bactericidal preservative, comprising the steps of:

[0035] (1) Take transglutaminase powder and add it to PBS buffer solution with a concentration of 0.2 mol / L at a mass ratio of 1:8, stir and mix at 30°C for 20 minutes to obtain a stirred mixture, take the stirred mixture and centrifuge at 3000 r / min for 10 minutes , take supernatant A for vacuum filtration, take the filtrate for dialysis, and freeze-dry to obtain dry powder A for subsequent use;

[0036] (2) Take sulfur and add sodium methoxide and methanol at a mass ratio of 6:11:25, stir and mix at -3°C for 1 hour, then add benzyl chloride with 50% methanol mass, heat up to 75°C and keep for 12 hours, cool, and filter with suction. Take the suction filtrate a for vacuum distillation, take the vacuum distillate a, add deionized water and diethyl ether in a mass ratio of 1:10:15, mix, let it stand, colle...

Embodiment 2

[0041] Transglutaminase powder: purchased from Wuhan Huashun Biotechnology Co., Ltd.

[0042] A preparation method of a bactericidal preservative, comprising the steps of:

[0043] (1) Take transglutaminase powder and add it to PBS buffer solution with a concentration of 0.2 mol / L at a mass ratio of 1:8, stir and mix at 32°C for 22 minutes to obtain a stirred mixture, take the stirred mixture and centrifuge at 3000 r / min for 10 minutes , take supernatant A for vacuum filtration, take the filtrate for dialysis, and freeze-dry to obtain dry powder A for subsequent use;

[0044] (2) Take sulfur and add sodium methoxide and methanol at a mass ratio of 6:11:22, stir and mix at -3°C for 1 hour, then add benzyl chloride with 52% methanol mass, raise the temperature to 77°C and keep for 13 hours, cool, and filter with suction. Take the suction filtrate a for vacuum distillation, take the vacuum distillate a, add deionized water and diethyl ether in a mass ratio of 1:10:15, mix, let i...

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Abstract

The invention belongs to the technical field of preservatives, and particularly relates to a preparation method of an antibacterial preservative. According to the method, by synthesizing a macromolecular antibacterial peptide substance, a long-chain hydrophilic-positive ion antibacterial peptide variant is prepared, wherein the macromolecular antibacterial peptide substance mainly consists of hydrophilic positive ion polypeptides and has selectively antibacterial activity, and by removing hydrophobicity, not only can the antibacterial activity and membrane activity similar to those of amphoteric macromolecules be preserved, but also hemolysis toxicity is even eliminated, and hydrophobicity in antibacterial peptide macromolecules is removed. On the one hand, an assistance is provided for breaking the stability of cell membranes and breaking the completeness of the cell membranes to generate holes, correspondingly cellular contents are exposed to the outside of cells and die, an effect of killing bacteria and resisting the bacteria is achieved, a broad-spectrum antibacterial capability is achieved, and most of germs can be killed; on the other hand, the hydrophilic performance of thepreservative can help to improve the water solubility, and the preservative has great biocompatibility and can form a stable antibacterial preservative liquid.

Description

technical field [0001] The invention belongs to the technical field of preservatives, and in particular relates to a preparation method of a bactericidal preservative. Background technique [0002] Fiber oil bactericidal preservative is a preparation for sterilizing chemical fiber oil, suitable for various chemical fiber oils, and widely used in POY, FDY, UDY and other oil systems. It is generally made of a variety of high-efficiency preservatives, which can kill a variety of bacteria, fungi, and yeast. [0003] In the production process of synthetic fibers, it is necessary to add an appropriate amount of oil agent. The oil agent is usually composed of various functional surfactants. Its main function is to endow fibers with excellent smoothness, antistatic properties, and clustering properties. The emulsion that constitutes the oil contains a lot of water, and at the same time contains a large number of essential elements for the growth of microorganisms. In addition, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N61/00A01N37/46A01N25/02A01N25/10A01P1/00A01P3/00
CPCA01N25/02A01N25/10A01N37/46A01N61/00
Inventor 黄宇成郑薇林凡
Owner CHANGZHOU DAAO NEW MATERIAL TECH CO LTD
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