Detection method and application of cardiomyocytes based on photonic crystal hydrogel fibers

A technology of photonic crystal fibers and photonic crystals, applied in the field of biomedical material research, to achieve the effects of easy large-scale production, simple detection methods, and low cost

Active Publication Date: 2020-03-31
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the research and development of a highly sensitive detection platform for cardiomyocytes, the preparation of materials capable of sensing at the single-cell level, and the construction of stable cardiomyocytes and detection technologies for the contractility and beating frequency of myocardial single cells still face severe challenges. challenge

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  • Detection method and application of cardiomyocytes based on photonic crystal hydrogel fibers
  • Detection method and application of cardiomyocytes based on photonic crystal hydrogel fibers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Detection and application of cardiomyocytes based on gelatin methacrylate (GelMA) photonic crystal hydrogel fibers

[0040] 1. Preparation of GelMA photonic crystal hydrogel fiber:

[0041] Photonic crystal hydrogel fibers were prepared by chemical etching method using photonic crystal fiber template and biocompatible hydrogel:

[0042] 1) first disperse the purified silicon dioxide particles monodispersed at 300nm in an ethanol solution to obtain a silicon dioxide ethanol solution with a concentration of silicon dioxide particles of 20wt%;

[0043] 2) Then inject the silica ethanol solution into the capillary (ID 300 μm), and naturally deposit to form a photonic crystal fiber template. Finally, the obtained photonic crystal fiber template is calcined at a high temperature (800°C) to obtain a photonic crystal fiber with good mechanical strength template;

[0044] 3) Soak the photonic crystal fiber template with better mechanical strength in the GelMA hydroge...

example 2

[0054] Example 2 A kind of cardiomyocyte detection based on bovine serum albumin (BSA) photonic crystal hydrogel fiber and its application

[0055] 1. Preparation of BSA photonic crystal hydrogel fiber:

[0056] Photonic crystal hydrogel fibers were prepared by physical dissolution method using photonic crystal fiber template and biocompatible hydrogel:

[0057] 1) Firstly, the purified polystyrene particles dispersed in 100 nm are dispersed in an aqueous solution to obtain a polystyrene aqueous solution with a concentration of 10 wt %;

[0058] 2) Then inject the polystyrene aqueous solution into the capillary (ID 200 μm), and naturally deposit to form the photonic crystal fiber template.

[0059] 3) Soak the photonic crystal fiber template with better mechanical strength in BSA hydrogel prepolymer solution (0.2g / ml) for 2h, and cross-link with glutaraldehyde (2wt%) to obtain the photonic crystal hydrogel hybrid system;

[0060] 4) Finally, absolute ethanol was used to diss...

example 3

[0069] Example 3 Detection and application of cardiomyocytes based on gelatin photonic crystal hydrogel fibers

[0070] 1. Preparation of gelatin photonic crystal hydrogel fibers:

[0071] Photonic crystal hydrogel fibers were prepared by chemical etching method using photonic crystal fiber template and biocompatible hydrogel:

[0072] 1) first disperse the purified silicon dioxide particles monodispersed at 200nm in an ethanol solution to obtain a silicon dioxide ethanol solution with a concentration of silicon dioxide particles of 2 wt %;

[0073] 2) Then inject the silica ethanol solution into the capillary (ID 200μm), and naturally deposit to form a photonic crystal fiber template, and finally perform high-temperature calcination (100°C) on the obtained photonic crystal fiber template to obtain a photonic crystal fiber with better mechanical strength template;

[0074] 3) Soak the photonic crystal fiber template with better mechanical strength in the gelatin hydrogel pre...

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Abstract

The invention discloses a myocardial cell detection method based on photonic crystal hydrogel fiber and application thereof; the method comprises the steps of 1) preparing biocompatible photonic crystal fiber; 2) using the photonic crystal hydrogel fiber to culture myocardial cells; 3) detecting the myocardial cells based on the photonic crystal hydrogel fiber; 4) analyzing data. The photonic crystal hydrogel fiber prepared herein has good biocompatibility; the photonic crystal hydrogel fiber has photonic band gap in photonic crystal, provides an optical sensing substrate for detection of myocardial cells, and contractibility and beat frequency of the myocardial cells are detected through changes in structural color or reflecting peak of the photonic crystal hydrogel fiber; the method of the invention is applicable to screening and evaluation of cardiac drugs, and screening is performed according to the changes in the contractibility and beat frequency of the myocardial cells after thedrugs are added. The method provided herein is simple to perform, low in cost, and convenient to apply to large-scale production.

Description

technical field [0001] The invention relates to a cardiomyocyte detection method based on a photonic crystal hydrogel fiber and an application thereof, belonging to the technical field of biomedical material research. Background technique [0002] At present, the source of cardiomyocytes mainly depends on the culture of cardiomyocytes in vitro and the construction of animal models in vivo. In the process of detecting the function of cardiomyocytes, simple two-dimensional cell culture cannot be real due to the lack of complexity of the body. Simulate complex physiological processes in the body; and the uncertainty of animal models and their own complexity make it also face certain challenges in the application of cardiomyocyte detection. In the development of new drugs and other physiological process research, for the In order to realize the true simulation of internal organs at the cell or organ level, researchers have combined the advantages of precision machining, bionics ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/25G01N33/50C12N5/077
CPCC12N5/0657C12N2533/00C12N2533/30C12N2533/50C12N2533/54C12N2533/74C12N2533/76C12N2533/78G01N21/25G01N21/255G01N33/5029G01N33/5061
Inventor 赵远锦付繁繁高崴陈卓玥刘羽霄
Owner SOUTHEAST UNIV
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