Preparation method for injection for treating diabetic wounds and product thereof

A technology of diabetes and injection, which is applied in the field of medicine, can solve the problems of weakened activity, weakened therapeutic effect, poor healing effect of diabetic refractory wounds, etc., and achieve the effect of enhanced effect

Active Publication Date: 2018-04-20
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mesenchymal stem cells have achieved some curative effects in the treatment of diabetic refractory wounds, after intravenous or local injection of a single MSCs cell, the cell survival rate will decrease and the activity will weaken during the migration process. Its therapeutic effect will be weakened, resulting in poor healing effect of diabetic refractory wounds in the later stage

Method used

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  • Preparation method for injection for treating diabetic wounds and product thereof
  • Preparation method for injection for treating diabetic wounds and product thereof
  • Preparation method for injection for treating diabetic wounds and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Cultivate umbilical cord mesenchymal stem cells in a culture dish up to passage 6, then add 1 ml of trypsin with a mass fraction of 0.25% to digest for 1 min, add an equal volume of serum after digestion to remove the activity of trypsin, and then carry out the first For the first centrifugation, the centrifugal force is 800g, and the time is 5min. After the centrifugation is completed, the supernatant is discarded, and then the cells are resuspended with 10ml PBS solution, and the second centrifugation is performed. The centrifugal force is 800g, and the time is 5min. supernatant, and then add 3ml PBS solution to resuspend the cells to obtain a concentration of 5×10 6 cells / ml solution containing mesenchymal stem cells (MSCs).

[0028] 2) Dissolve 200ug type I collagen in 1ml of 0.5% acetic acid aqueous solution by mass volume ratio to obtain a type I collagen solution; dissolve 100ug laminin in 1ml PBS solution to obtain a laminin solution; dissolve the type I coll...

Embodiment 2

[0030] Example 2 Application of Injection for Treating Diabetic Wounds in Treating Diabetic Wounds in Rats

[0031] Rats fed with high sugar and high fat were taken, and 35 mg / kg streptozotocin (STZ) was injected intraperitoneally to establish a diabetic rat model. The modeled diabetic rats were randomly divided into three groups. A full-thickness skin defect wound with a diameter of 1 cm was created on the upper part, and each group was injected with local subcutaneous injection around the wound. The control group was injected with 1ml of PBS solution, the MSCs treatment group was injected with 1ml of the MSCs solution prepared in Example 1, and the ECM-MSCs treatment group was injected with 1ml The ECM-MSCs injection prepared in Example 1.

[0032] The size of the wound was measured at 2, 4, 6, 8, 10, and 12 days after the injection to observe the healing situation; HE and immunohistochemical staining were performed on the healed diabetic wound to observe the wound repair an...

Embodiment 3

[0040] 1) Cultivate umbilical cord mesenchymal stem cells in a culture dish to passage 7, then add 1ml of trypsin with a mass fraction of 0.1% to digest for 2 minutes, add an equal volume of serum after digestion to remove the activity of trypsin, and then carry out the first For the first centrifugation, the centrifugal force is 800g, and the time is 5min. After the centrifugation is completed, the supernatant is discarded, and then the cells are resuspended with 10ml PBS solution, and the second centrifugation is performed. The centrifugal force is 800g, and the time is 5min. supernatant, and then add 5ml PBS solution to resuspend the cells to obtain a concentration of 8×10 6 cells / ml solution containing mesenchymal stem cells (MSCs).

[0041] 2) Dissolve 100ug type I collagen in 1ml of 1.0% acetic acid aqueous solution by mass volume ratio to obtain a type I collagen solution; dissolve 38ug laminin in 1.5ml PBS buffer solution to obtain a laminin solution; The collagen sol...

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Abstract

The invention discloses a preparation method for an injection for treating diabetic wounds and a product thereof. The preparation method comprises the following steps: (1) a solution which contains mesenchymal stem cells is prepared; (2) a mixed solution of type I collagen solution and laminin is prepared; (3) the mixed solution and the solution containing the mesenchymal stem cells are mixed andsufficiently and uniformly dispersed, and thereby the injection for treating diabetic wounds is obtained. According to the invention, the type I collagen and the laminin are mainly utilized to wrap the mesenchymal stem cells, the activity of the mesenchymal stem cells is protected, and thereby the problem that the therapeutic effect is weakened as time goes by in the process of treatment is avoided; moreover, the type I collagen and the laminin can cooperate with the mesenchymal stem cells, and thereby the therapeutic effect is further enhanced.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of an injection for treating diabetic wounds and a product thereof. Background technique [0002] Wound healing is a complex biological process. It is a complex process in which inflammatory cells, repair cells, extracellular matrix, cytokines and other factors participate in and are highly coordinated and mutually regulated. The skin of diabetic patients is vulnerable to damage. After the damage, it often recurs and persists, forming stubborn and difficult-to-heal ulcers. Glycosylation, causing abnormal function of vascular endothelium and impaired response; abnormal function of macrophages, prolonged and unbalanced inflammatory response; lack or damage of growth factors, reduction of gelatin tissue, disorder of epidermal barrier function, and reduction of granulation tissue, etc. , resulting in ulcers that are difficult to heal. With the impr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/51A61K9/08A61K47/42A61P17/02A61P3/10A61K38/39
CPCA61K9/0019A61K9/08A61K35/51A61K38/39A61K47/42A61K2300/00
Inventor 莫朝晖汪凌昊何红晖赵立玲陈科万新星
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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