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Tissue engineered cornea

A tissue engineering, corneal technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problem of reduced expression, and achieve the effect of improving thickness and mechanical toughness

Inactive Publication Date: 2018-04-20
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been confirmed that keratocytes cultured in vitro can easily differentiate into fibroblasts and further differentiate into fibroblasts, resulting in decreased expression of keratocyte-specific proteins such as keratin, CD34 and CHST6

Method used

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Examples

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Embodiment Construction

[0029] The technical solutions of the present invention will be further illustrated and described below through specific embodiments in conjunction with the accompanying drawings.

[0030] The ultra-thin amniotic membrane in the following examples is the dense layer retained after the spongy layer and reticular layer are removed by protease digestion.

[0031] Materials and methods:

[0032] 1. Multi-layer ultra-thin amniotic membrane production process (such as figure 1 shown)

[0033] (1) The placenta comes from a cesarean section patient, with the consent of the puerpera and her family members and signed a consent form, and the test for AIDS, syphilis, hepatitis B, hepatitis C and other pathogens before the cesarean section is negative; in the ultra-clean workbench, the Cut the amniotic membrane with chorion from the placenta; tear off the chorion; rinse repeatedly with sterile PBS solution until the amniotic membrane is smooth and transparent without blood stains to obta...

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Abstract

The invention discloses a tissue engineered cornea. The tissue engineered cornea comprises at least three amnion tissue layers, wherein corneal stromal cells are grown between two adjacent amnion tissue layers, extracellular matrixes which are secreted by the corneal stromal cells and contain keratocan, lumican, I type collagen and V type collagen are also contained, and the extracellular matrixesare regularly arranged, so that a tissue structure similar to corneal stroma is formed. Corneal epithelial cells and corneal endothelial cells are respectively inoculated on the upper surface and thelower surface of the structure, so that a total cornea is formed. Surface collagenous fibers of the amnion tissue layers are regularly arranged, are parallel to each other and are transversally intersected and closely arranged to form a fiber sheet, so that the corneal stromal cells and the extracellular matrixes secreted by the corneal stromal cells are guided to be regularly arranged; and at least three amnion tissue layers have the characteristic of inhibiting fibrosis, so that the characteristics of the corneal stromal cells are maintained, and differentiation of the corneal stromal cellsto fibroblasts is inhibited.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a tissue engineering cornea. Background technique [0002] The human corneal stroma is composed of tightly arranged collagen fiber sheets, which have high transparency and good mechanical toughness, which are difficult to achieve in vitro, so it is a great challenge for tissue engineering cornea in vitro. About 285 million people are visually impaired worldwide, of which 39 million are blind. Corneal disease is the second leading cause of blindness after cataracts. At the same time, it is reported that about 20% of the causes of blindness in children are caused by corneal diseases. At present, when other treatment methods fail, corneal transplantation is the most effective and common treatment for corneal diseases, but due to the shortage of high-quality corneal donors, rejection after corneal transplantation, and the increasing failure rate after transpla...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/36A61L27/38
CPCA61L27/3604A61L27/3687A61L27/3834A61L2430/16
Inventor 李炜车欣张丽颖何卉刘祖国
Owner XIAMEN UNIV
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