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Method of extracting anthocyanin of blackberries by adopting eutecticevaporate solvent

A deep eutectic solvent and anthocyanin technology, which is applied in the field of food processing, can solve the problems of different extraction effects, and achieve the effects of mild reaction conditions, easy operation, and wide application range

Inactive Publication Date: 2018-04-20
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are great differences in the composition and structure of anthocyanins in different plant raw materials, and the extraction effects are also different.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In this embodiment, blackberry is used as anthocyanin raw material.

[0022] A method for extracting blackberry anthocyanins using a deep eutectic solvent, comprising the following steps:

[0023] (1) After the blackberry is homogenized, it is dried in a drying oven at 40° C., pulverized, and passed through an 80-mesh sieve;

[0024] (2) Accurately weigh 1.0000 g of blackberry powder after sieving, mix with 10 mL of deep eutectic solvent composed of choline chloride and malic acid with a molar ratio of 1.5: 1 according to the solid-liquid ratio of 1: 10, add 35 % of water; ultrasonically assisted extraction, the extraction power is 300W, the temperature is 45°C, and the time is 30min; after the reaction is completed, a deep eutectic solvent solution system containing anthocyanins is obtained. Centrifuge at 4000rpm for 20min, take the supernatant, ie the crude anthocyanin extract; filter through a 0.45μm filter membrane to obtain the fine anthocyanin extract.

Embodiment 2

[0026] In this embodiment, blackberry is used as anthocyanin raw material.

[0027] A method for extracting blackberry anthocyanins using a deep eutectic solvent, comprising the following steps:

[0028] (1) After the blackberry is homogenized, it is dried in a drying oven at 40° C., pulverized, and passed through an 80-mesh sieve;

[0029] (2) Accurately weigh 1.0000 g of blackberry powder after sieving, mix with 30 mL of choline chloride and proline with a molar ratio of 1: 1 according to the solid-liquid ratio of 1: 30 and 30 mL, and add Diluted with 35% water; ultrasonically assisted extraction, the extraction power is 300W, the temperature is 45°C, and the time is 30min; after the reaction is completed, a deep eutectic solvent solution system containing anthocyanin is obtained. Centrifuge at 4000rpm for 20min, take the supernatant, namely the crude anthocyanin extract; filter through a 0.22μm filter membrane to obtain the fine anthocyanin extract.

Embodiment 3

[0031] In this embodiment, blackberry wine residue is used as anthocyanin raw material.

[0032] A method for extracting blackberry anthocyanins using a deep eutectic solvent, comprising the following steps:

[0033] (1) Blackberry wine residue is dried in a drying oven at 40°C, pulverized, and passed through a 100-mesh sieve;

[0034] (2) Accurately weigh 1.0000 g of sieved blackberry wine lees powder, mix it with 15 mL of deep eutectic solvent composed of choline chloride and oxalic acid with a molar ratio of 1:1 according to the solid-liquid ratio of 1:15, add 34.8 % of water; ultrasonically assisted extraction, the extraction power is 330W, the temperature is 45°C, and the time is 30min; after the reaction is completed, a deep eutectic solvent solution system containing anthocyanins is obtained. Centrifuge at 4000rpm for 20min, take the supernatant, ie the crude anthocyanin extract; filter through a 0.45μm filter membrane to obtain the fine anthocyanin extract.

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PUM

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Abstract

The invention discloses a method of extracting anthocyanin of blackberries by adopting an eutecticevaporate solvent. The method comprises the following steps: drying, crushing and sieving a blackberryraw material; mixing the sieved blackberry raw material with the eutecticevaporate solvent, adding water to dilute, carrying out ultrasonic assisted extraction to obtain an eutecticevaporate solventsystem containing anthocyanin, carrying out centrifugalization, and taking the supernate to obtain an anthocyanin coarse extraction solution; and filtering the coarse extraction solution through a filter membrane to obtain an anthocyanin fine extraction solution. The method provided by the invention has the advantages of being green and safe in extraction solvent, mild in reaction condition, easyto operate, efficient to extract and the like, and is suitable for extracting anthocyanin matters in a plant raw material.

Description

technical field [0001] The invention belongs to the field of food processing, in particular to a method for extracting blackberry anthocyanins by using a deep eutectic solvent. Background technique [0002] Blackberries are known as the "fruit of life". Blackberry anthocyanins include cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, cyanidin-3-O-xyloside, cyanidin-3-O-malonate glucoside Glycosides, etc., wherein the content of cyanidin-3-O-glucoside is as high as 80%. The extraction methods of blackberry anthocyanins reported in relevant literatures include water extraction, organic solvent extraction, biological enzymatic hydrolysis, ultrasonic and microwave-assisted methods, etc. Due to the common shortcomings of traditional extraction methods such as low efficiency, residual organic solvents, and easy destruction of anthocyanins during the extraction process, it is urgent to explore the application of some new methods in the extraction of anthocyanins from blackberries...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 范琳琳周剑忠夏秀东
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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