Preparation method for carbon quantum dot with high fluorescence quantum yield and application of carbon quantum dot in cell imaging

A technology of fluorescence quantum yield and carbon quantum dots, applied in the field of nanomaterials, can solve the problems of low quantum yield, not reaching quantum yield, etc., and achieve the effects of simple synthesis method, superior biocompatibility, and high fluorescence yield

Inactive Publication Date: 2018-04-20
WUHAN UNIV OF TECH
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the quantum yields of currently synthesized carbon quantum dots are relatively low, such as: the quantum yield of yellow quantum dots synthesized by Li et al. is 11.7%, even after sodium borohydride reduction, the quantum yield reaches 22.9%; After the quantum dots were connected to alkaneamides through chemical reactions, the quantum yield increased from 0.02% to 13%; Tesuka et al. modified carbon quantum dots with amino groups and the yield reached more than 40%. In addition, there are many different types of synthetic carbon quantum dots, but they are nowhere near the quantum yield one would expect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for carbon quantum dot with high fluorescence quantum yield and application of carbon quantum dot in cell imaging
  • Preparation method for carbon quantum dot with high fluorescence quantum yield and application of carbon quantum dot in cell imaging
  • Preparation method for carbon quantum dot with high fluorescence quantum yield and application of carbon quantum dot in cell imaging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method with high fluorescence quantum yield carbon quantum dots, comprising the steps of:

[0035] (1) Dissolve 1.5 g of citric acid monohydrate and 0.57 g of L-cysteine ​​in 10 mL of deionized water, and fully dissolve to obtain a precursor solution;

[0036] (2) Move the obtained precursor solution to a microwave oven, heat the reaction for 4 minutes at a power of 1300W, and naturally cool to room temperature to obtain a paste solid;

[0037] (3) Add 10 mL of water to dissolve the paste solid obtained in step (2) to obtain a suspension; then centrifuge the suspension at 9000r / min for 10 minutes in a high-speed centrifuge, take the supernatant, and use a 0.22 μm needle to remove the supernatant Filter through a pore filter and collect the filtrate, which is the carbon quantum dot solution;

[0038] (4) Dry the carbon quantum dot solution under vacuum conditions (the drying temperature is 30° C., and the drying time is 32 h), to obtain the carbon quantum ...

Embodiment 2

[0042] A cell biological experiment with high fluorescence quantum yield carbon quantum dots, comprising the following steps:

[0043] (1) Starve the HUVEC cells and CRL-5288 cells for a certain period of time;

[0044] (2) get the carbon quantum dots prepared in embodiment 1, be made into different concentrations, add respectively in the petri dish of two kinds of cells after filter sterilization, cultivate;

[0045] (3) Detecting the situation of the carbon quantum dots prepared in Example 1 of cell phagocytosis under a fluorescence microscope;

[0046] (4) After culturing for 6h, 24h, and 48h, the absorbance values ​​were detected respectively, and the inhibition rate of the carbon quantum dots prepared in Example 1 on cells was calculated according to the formula.

[0047] It can be seen from this example that: at the optimal working concentration (0.07825mg / mL) of the carbon quantum dots prepared in Example 1, the cell inhibition rate is the lowest and less than 6% in th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method for a carbon quantum dot fluorescent probe used for detection of the concentration of nitric oxide in organisms. The carbon quantum dot is a powdery solidwith excellent water solubility, is synthesized from citric acid and L-cysteine through a microwave-assisted method, and has extremely high quantum yield which is up to 85%. The preparation method for the carbon quantum dot comprises the following steps: 1) dispersing and dissolving the citric acid and the L-cysteine in deionized water; 2) carrying out stirring and ultrasonic dispersion, and allowing the citric acid and the L-cysteine to be uniformly dispersed in a solution; 3) transferring a mixed solution into a microwave oven, and carrying out heating for 2 to 8 min; 4) carrying out cooling to a room temperature, adding water, and carrying out dissolving; 5) transferring an obtained solution into a centrifuge, and carrying out centrifuging at a high speed; and 6) subjecting a centrifuged solution to filtering and drying operations so as to prepare the carbon quantum dot. The carbon quantum dot prepared by using the method provided by the invention has the advantages of high fluorescence quantum yield, good chemical stability, photobleaching resistance and biological compatibility, and applicability to detection of the concentration of the nitric oxide in the organisms.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and in particular relates to a preparation method of carbon quantum dots with high fluorescence quantum yield and its application in cell imaging. Background technique [0002] Carbon is one of the earliest elements human beings came into contact with, and it is also one of the most common elements on earth. Amino acids and nucleotides, the basic units of life on earth, come from carbon as the skeleton, and have been highly concerned throughout the development of human science and technology. Carbon atoms have a variety of electron orbits, which can form many substances with different structural properties, so there are many allotropes of carbon in nature, such as diamond, graphite, graphene, carbon nanotubes, etc. Carbon quantum dots were accidentally discovered by Xu et al. in the purification of single-layer carbon nanotubes in 2004, which triggered a new wave of research on fluorescent nan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B32/15A61K49/00B82Y20/00B82Y40/00
CPCA61K49/0019B82Y20/00B82Y40/00C09K11/65
Inventor 丁莉芸林海涛黄俊王星瑞
Owner WUHAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap