Cell digester

Abstract

The invention provides a cell digester. The cell digester comprises a digestion tank, a bracket used for supporting the digestion tank, and a digestion tank top cover, wherein the digestion tank comprises a tank body and a tank bottom; the tank bottom adopts a cavity for collecting liquid; a liquid outlet is formed in the lowest end of the cavity; a detachable filter screen is horizontally arranged in a position where the tank body is connected with the tank bottom; the filter screen is matched with the tank body, and can intercept microcarriers or impurity block masses and ensure that cells flow to the cavity of the tank bottom; a sampling / recycling opening is formed in the outer side wall of the tank body; the sampling / recycling opening is formed above the filter screen and is used for sampling or recycling microcarriers. The cell digester can be used for digestion of primary cells and digestion of microcarrier culture cells, can separate cells, microcarriers or large block masses inreal time during digestion, greatly shortens the cell digestion period and prevents basal cells from being pressed, is beneficial to continuous cell culture, and is applicable to industrial and scalecell culture.

Description

technical field [0001] The invention relates to a cell digester. Background technique [0002] During the preparation of primary cells, the tissue needs to be cut into small pieces, which are then digested with a digestive agent into tissue clumps and then into individual cells. During this process, cells are gradually dissociated from the tissue and become a single-cell suspension. The existing digestion technology cannot collect cells and terminate digestion in real time, which greatly damages the biological characteristics of cells, resulting in low cell adhesion rate and difficulty in surviving. [0003] In the field of biological products, when preparing cell seeds and cell culture, cells are usually seeded on microcarriers and cultured in fermenters. The most important thing in the process of cell fermentation is the digestion of cells. However, the drain ports of various fermentors disclosed in the prior art are all above the bottom of the fermenter. When the cells...

AI Technical Summary

Problems solved by technology

However, the sampling ports of commercially available fermenters are all higher than the bottom of the fermenter, resulting in the need to add excess digestive juice to increase the volume so that the liquid level is higher than the sampling port to take samples

As we all know, excessive digestive juice can easily cause irreversible damage to cells, thereby changing the biological characteristics of cells, resulting in low survival rate of cells in the subsequent culture process, and even affecting the quality of biological products

However, after cell digestion, microcarriers and cells can’t be effectively separated by precipitation at the same time, so it is extremely difficult to apply to the preparation of cell seeds.

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