High-salt-tolerance nucleic acid sensor for mercury and application of high-salt-tolerance nucleic acid sensor

Abstract

The invention discloses a high-salt-tolerance nucleic acid sensor for mercury and an application of the high-salt-tolerance nucleic acid sensor. The sensor comprises a molecular identification and signal amplification element and a signal transformation element, wherein the molecular identification and signal amplification element is used for specifically identifying mercury ions, and an amplification target product is obtained under the action of the mercury ions by an isothermal exponential amplification reaction on the basis of mispairing of thymine-mercury ion-thymine; the amplification product forms a G-quadruplex structure under the action of heme, and accordingly, a concentration signal of the mercury ions is amplified and transformed into a concentration signal of the G-quadruplexstructure; the G-quadruplex structure of the signal transformation element is used for catalyzing color development of a color developer, and accordingly, the concentration signal of the mercury ionsis converted into a signal of optical density; the concentration of the mercury ions is calculated by detecting the optical density. The sensor has high specificity and sensitivity, and the mercury ions in high-salt field environment can be detected rapidly, qualitatively and quantitatively.

Description

technical field [0001] The invention belongs to the technical field of heavy metal detection, and in particular relates to a mercury high-salt-resistant nucleic acid sensor and an application thereof. Background technique [0002] Mercury is a highly toxic heavy metal element. It is usually silvery white. It is the only liquid metal at room temperature. It has fluidity and can evaporate at room temperature. It is commonly known as "mercury". Mercury is a very rare element in the earth's crust. Mercury in nature is mainly in the form of elemental mercury (Hg), inorganic mercury (Hg + , Hg 2+ Salt and its complexes) and organic mercury (alkylmercury, phenylmercury), various forms of mercury can undergo a variety of biological and chemical transformations in nature. Mercury rarely occurs in nature as a pure metal, with mercuric sulfide, thirmalite, mercurite, and minerals associated with cinnabar being the most common deposits of mercury. Mercury can be polluted through vari...

AI Technical Summary

Problems solved by technology

However, the traditional method of colorimetric detection of mercury still has certain limitations, such as cumbersome operation steps, poor sensitivity and selectivity, and the need to use organic solvents that easily pollute the environment, and many detection environments have high levels of salt. Therefore, it is urgent to develop a non-polluting, simple, fast, high sensitivity and high specificity method to meet the needs of trace metal mercury detection.

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