Susceptible gene chip used for detecting type II diabetes associated with organophosphorus pesticides

Abstract

The invention belongs to the technical field of gene detection and discloses a susceptible gene chip used for detecting type II diabetes associated with organophosphorous pesticides. The susceptible gene chip includes a stationary phase carrier made of aldehyde-based glass and specific oligonucleotide probes used for detecting type II diabetes susceptible genes associated with organophosphorus pesticide residues; nucleotide sequences of the specific oligonucleotide probes are shown as SEQ ID NO: 1 to SEQ ID NO: 8, and the probes are fixed to the stationary phase carrier. A complementary base specific hybridization method is utilized to achieve high-throughput detection; the operation is simple; after labeling is conducted by fluorescent dye, films are washed, a detection result is obtainedthrough a fluorescence scanner, coloration is not needed, the detection is rapid, the cost is low, and a trace reaction system is achieved. The detection result shows that the false positive rate is3.25% and the false negative rate is 1.05%. The susceptible gene chip has a great application market and economic benefits and is easy to apply on a large scale.

Description

technical field [0001] The invention belongs to the technical field of gene detection, in particular to a susceptibility gene chip for detecting type II diabetes related to organophosphorus pesticides. Background technique [0002] Diabetes is a common endocrine and metabolic disease characterized by chronic hyperglycemia. The most common type is type II diabetes, accounting for about 90% of the total number of diabetes. The etiology and pathogenesis of type Ⅱ diabetes are complex, and it has obvious genetic susceptibility. It is currently considered to be a multifactorial disease caused by the participation and interaction of multiple risk factors such as genetics, environment, and behavior. In recent years, with the completion of the Human Genome Project and the establishment of high-throughput single nucleotide polymorphism (single nucleotide polymorphism, SNP) detection gene chips, a series of susceptibility genes related to type 2 diabetes have been discovered. So far,...

AI Technical Summary

Problems solved by technology

[0005] Diabetes susceptibility gene detection methods generally use PCR-RFLP and DNA sequencing methods in the past, but there are certain shortcomings, such as long operation time, cumbersome steps, easy missed detection, and not suitable for batch, multi-locus, special structural sequence detection, etc. Difficulties in the clinical diagnosis of diabetes

At present, there is still a lack of type 2 diabetes susceptibility gene detection chip related to organophosphorus pesticides. There is no correlation between the existing type 2 diabetes susceptibility gene detection gene chip and type 2 diabetes caused by long-term exposure to organophosphate pesticides. Basis for selection of targeted therapy drugs

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