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Method for accurately measuring content of chitosan oligosaccharide according to resonance rayleigh scattering method

A technology of resonance Rayleigh scattering and accurate measurement, which can be used in measurement devices, material analysis by optical means, instruments, etc., and can solve the problems of increased resonance scattering intensity and cumbersome operation techniques.

Active Publication Date: 2018-05-29
GUANGDONG PHARMA UNIV
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Problems solved by technology

[0005] In order to overcome the technical deficiency that the content of chitosan oligosaccharides in the finished product is difficult to quantify in the prior art and the operation technology is cumbersome, the present invention provides a method for accurately measuring the content of chitosan oligosaccharides by the resonance Rayleigh scattering method. , chitosan oligosaccharides can combine with ruby ​​red to form ionic associations, which significantly enhance the resonance scattering intensity, adding sodium dodecyl sulfate as a sensitizer to improve its sensitivity

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  • Method for accurately measuring content of chitosan oligosaccharide according to resonance rayleigh scattering method
  • Method for accurately measuring content of chitosan oligosaccharide according to resonance rayleigh scattering method
  • Method for accurately measuring content of chitosan oligosaccharide according to resonance rayleigh scattering method

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Embodiment

[0072] A method for accurately measuring chitosan content by resonance Rayleigh scattering method, comprising the steps of:

[0073] 1) Draw the standard curve of △I and different concentrations of chitosan oligosaccharides

[0074] Add 1.0mL of B-R buffer solution with a pH of 4.9, a certain concentration gradient of chitosan oligosaccharide standard solution, 1.0mL of sodium lauryl sulfate solution with a concentration of 0.086mol / L, and 1.0mL of a concentration of 1.0× 10 -4 mol / L ruby ​​red solution, use distilled water to make up the volume, shake well, place it at room temperature for 10min, and then measure it on the F-2500 fluorescence spectrophotometer with λ ex =λ em Carry out synchronous scanning, record the resonance scattering intensity I of each detection system containing chitosan oligosaccharide at λ=470nm, and the resonance scattering intensity I of the reagent blank at λ=470nm 0 , and calculate △I=I-I 0 , Establish the standard curve C of ΔI and chitosan ...

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Abstract

The invention relates to a method for accurately measuring the content of chitosan oligosaccharide according to a resonance rayleigh scattering method, and belongs to the field of macromolecule detection. The chitosan oligosaccharide and rubylith are combined to form ionic associate under the certain condition, then the resonance scattering strength is obviously increased, and lauryl sodium sulfate serving as a sensitizer is added to improve the sensitivity. The resonance scattering strength and the chitosan oligosaccharide form a linear relation within certain concentration range, the linearrelation is taken as a quantitative basis of the chitosan oligosaccharide, and the resonance rayleigh scattering method for measuring the chitosan oligosaccharide is established. The measurement of the method is affected by the ionic concentration and the interferent, a standard product with the ionic concentration controlled to 0.05 mol / L to 0.20 mol / L is taken as a quantitative standard in the actual measurement of a sample, and the method has the advantages of cheap agents, high sensitivity, good repeatability, simplicity and convenience in operation, and the like.

Description

technical field [0001] The invention relates to a method for accurately measuring chitosan oligosaccharide content by a resonance Rayleigh scattering method, belonging to the field of macromolecular detection. Background technique [0002] Chitosan oligosaccharide / chitooligosaccharide (COS), also known as low oligoglucose, low oligoglucosamine, etc., is a basic amino oligosaccharide, which is formed by connecting 2-glucosamine through β-1,4 glycosidic bonds. Which contains part of the acetylglucosamine residues. Oligochitosan has a wide range of sources, is safe and non-toxic, has good water solubility, and has various biological activities such as anti-oxidation, antibacterial, anti-tumor, and enhancement of immunity. It has attracted widespread attention from domestic and foreign medical researchers. Therefore, the accurate quantification of chitosan oligosaccharides is very important, and it is of great significance to establish a simple and efficient method to determine...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 白研苏政权陈红红
Owner GUANGDONG PHARMA UNIV
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