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Application of 20S proteasome inhibitor in preparing medicine for treating Japanese B encephalitis virus infection

A proteasome inhibitor, Japanese encephalitis virus technology, applied in the field of medicine

Active Publication Date: 2018-06-01
武汉百药联科科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no precedent for the use of bortezomib, carfilzomib, and exazomib in the treatment of Japanese encephalitis virus infection

Method used

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  • Application of 20S proteasome inhibitor in preparing medicine for treating Japanese B encephalitis virus infection
  • Application of 20S proteasome inhibitor in preparing medicine for treating Japanese B encephalitis virus infection
  • Application of 20S proteasome inhibitor in preparing medicine for treating Japanese B encephalitis virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Application of bortezomib in the treatment of mice infected with Japanese encephalitis virus

[0037] 1. Establishment of a mouse model infected with Japanese encephalitis virus

[0038] 1. Experimental materials

[0039] (1) Experimental animals: four-week-old Balb / c female mice.

[0040] (2) Virus strain: JEV P3 strain

[0041] (3) Other reagents:

[0042] 1) 0.01M PBS buffer: weigh NaH 2 PO 4 0.593g, Na 2 HPO 4 5.802g, NaCl 17.0g, deionized water (ddH 2 O) Dilute to 2L, store at room temperature for later use.

[0043] 2) DMEM basal medium: a bottle of DMEM powder, weighed NaHCO 3 3.7g, HEPES 5.95g, add 800mLddH 2 O, stir well to dissolve, dilute to 1000mL, sterilize with a 0.22μm filter, and store at 4°C for later use.

[0044] 2. Experimental steps

[0045] Mice were injected intraperitoneally with 10 6 PFU Japanese encephalitis virus, the mice in the control group were intraperitoneally injected with the same dose of DMEM medium. At the su...

Embodiment 2

[0065] Example 2 Application of Carfilzomib in the Treatment of Mice Infected with Japanese Encephalitis Virus

[0066] 1. Establishment of a mouse model infected with Japanese encephalitis virus

[0067] The steps of establishing a mouse model infected with Japanese encephalitis virus are the same as in Example 1, and the therapeutic effect of carfilzomib on mice infected with Japanese encephalitis virus is as follows:

[0068] 2. Evaluation of the therapeutic effect of carfilzomib on mice infected with Japanese encephalitis virus

[0069] 1. Experimental materials

[0070] (1) Drug: Carfilzomib, traits: white powder, method of use: use 2% DMSO+30% PEG 300+2% Tween 80+ddH 2 O is dissolved and administered intravenously.

[0071] (2) Other reagents: 4% paraformaldehyde.

[0072] 2. Specific experimental steps

[0073] (1) Animal experiment grouping and sample collection:

[0074] Four-week-old Balb / c female mice were divided into four groups: PBS group (blank control gro...

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PUM

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Abstract

The invention discloses application of a 20S proteasome inhibitor in preparing a medicine for treating Japanese B encephalitis virus infection. The 20S proteasome inhibitor is a boric acid type 20S proteasome inhibitor or an epoxy ketone type 20S proteasome inhibitor. The boric acid type 20S proteasome inhibitor or the epoxy ketone type 20S proteasome inhibitor can effectively treat the Japanese Bencephalitis virus infection, and provides a new option for clinical medication. According to the application disclosed by the invention, by establishing an animal model of mice infected with the Japanese B encephalitis virus, analyzing the survival rate of the medicated mice and the pathological sections of the brain tissues, comparing with healthy mice and the mice not medicated after infectedwith the Japanese B encephalitis virus and conducting statistical data analysis, the 20S proteasome inhibitors such as bortezomib, ixazomib and carfilzomib are proven to have a certain treatment effect on the Japanese B encephalitis virus infection in the aspects of survival rate, brain tissue lesion and the like.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the use of a 20S proteasome inhibitor in the preparation of a medicine for treating Japanese encephalitis virus infection. Background technique [0002] Japanese encephalitis virus (Japanese encephalitis virus, JEV) belongs to Flaviviridae Flavivirus genus, is the pathogen that causes nervous system infectious disease - Japanese encephalitis, other members of the same genus are West Nile virus (West Nile virus) virus, WNV), yellow fever virus (Yellowfever virus, YFV), dengue virus (DENV) 1-4 types, Zika virus (Zika virus, ZIKV), etc. The virus is mainly transmitted through the bites of mosquitoes carrying the virus, and Culex tritaeniorhynchus is the main vector of the virus. Therefore, the prevalence of the virus shows obvious seasonality, with frequent occurrence in summer and autumn. [0003] Japanese encephalitis virus mainly infects pigs, horses, cattle and other animals a...

Claims

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Application Information

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IPC IPC(8): A61K38/05A61K38/08A61P31/14
CPCA61K38/05A61K38/08Y02A50/30
Inventor 张红雨吕博敏全源张青叶黄清
Owner 武汉百药联科科技有限公司
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