Plant growth-promoting bacillus aryabhattai for heavy saline-alkali soil and application of PGPR bacillusaryabhattai

A Bacillus arborii and rhizosphere growth-promoting technology, applied in the field of agricultural and forestry microorganisms, can solve the problems of reduced salt tolerance, reduced plant rhizosphere biological activity, and lack of resistance to high salt, so as to promote growth and improve salt-alkali tolerance Ability, improve the effect of salt-alkali resistance

Active Publication Date: 2018-06-01
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most plants and plant rhizosphere growth-promoting bacteria do not have the ability to resist high salinity and high salinity, especially in the case of severe saline-alkali

Method used

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  • Plant growth-promoting bacillus aryabhattai for heavy saline-alkali soil and application of PGPR bacillusaryabhattai
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  • Plant growth-promoting bacillus aryabhattai for heavy saline-alkali soil and application of PGPR bacillusaryabhattai

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening, isolation and preservation of rhizosphere growth-promoting bacteria in heavy saline-alkali soil

[0024] Collect 6 samples of Tamarix rhizosphere soil in the Yellow River Delta. The bacterial isolate was separated from the rhizosphere soil sample by gradient dilution method. Purify the isolated bacteria by the three-zone streaking method, and judge whether the strains are purified by microscopy, number the purified bacteria, pick a single colony, and inoculate it on a skimmed milk medium plate with a sterilized toothpick. After culturing at 37°C for 2 days, select the colonies with transparent circle, measure the colony diameter d and transparent circle diameter D, and calculate D / d, select the larger ratio for purification and repeatedly verify the effect of protease production, select the stable functional strain for further Re-screening.

[0025] Folin phenol method is used for re-screening. Add 1 mL of the original fermentation broth to 2 mL of 0....

Embodiment 2

[0026] Example 2 Physiological, biochemical and molecular identification of rhizosphere growth promoting bacteria GPR018 in heavy saline-alkali soil

[0027] Colony characteristics and cell morphology: the colony is round and regular, the colony is large, the surface is convex, relatively moist, slightly yellow, opaque, the cell is small, rod-shaped, with spores, and its colony morphology is like figure 1 As shown, the optical microscope inspection is as figure 2 Shown.

[0028] Physiological and biochemical characteristics: positive contact enzyme test, positive citrate utilization test, negative starch hydrolysis test, negative VP determination test, negative D-glucose test, negative D-mannitol test, negative trehalose test, negative D-sorbitol test , Sucrose test is negative, glucose gas production test is negative.

[0029] Functional qualitative and quantitative determination: add 1mL of the original fermentation broth to 2mL of 0.5% casein solution, bath in a water bath at pH ...

Embodiment 3

[0034] Example 3 The salt tolerance of GPR018 and the growth-promoting effect in a heavy salt-alkali environment

[0035] 1. Salt tolerance

[0036] Choose 4 species of Bacillus asheri, namely Bacillus asheri BPR078 (the deposit number is (CGMCC No.11971), Bacillus asheri BPR088 (the deposit number is (CGMCC No.11972)), and Bacillus asheri JIA2 (the deposit number is (CGMCC No.11967) and the present invention’s Bacillus argentii GPR018 (preservation number is (CGMCC No.11973). The 4 long-producing strains were first inoculated into liquid LB medium, 180rpm, 37℃ constant temperature shaker culture 24h . Then take 200μL of activated bacteria solution to the sodium chloride concentration of 1%, 3%, 6%, 9% LB liquid medium, each gradient three parallel, and then placed at 180rpm, 37 ℃ constant temperature shaking Incubate in a bed for 24 hours, use a spectrophotometer, and take the corresponding medium without inoculation of the bacterial suspension as a blank control, take regular sa...

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Abstract

The invention discloses plant growth-promoting bacillus aryabhattai for heavy saline-alkali soil and an application of the plant growth-promoting bacillus aryabhattai. The strain can improve salt andalkali resistance of tamarix chinensis in a heavy saline-alkali environment. A microbial agent can be prepared from the plant growth-promoting bacillus aryabhattai and inoculated to the periphery of the roots of the tamarix chinensis; compared with different inoculation microbial agents, the microbial agent can promote growth of the tamarix chinensis and increase water content of the roots and plants of the tamarix chinensis after salt stress for a certain period of time. Therefore, the bacillusaryabhattai can be used as an inoculant in a heavy saline-alkali habitat, and an excellent strain resource for improving saline-alkali resistance capacity of the tamarix chinensis is provided.

Description

Technical field [0001] The invention belongs to the technical field of agricultural and forestry microorganisms, and specifically relates to a plant rhizosphere growth-promoting Bacillus asheri and its application. Background technique [0002] Land salinization is one of the important factors restricting the development of agriculture and forestry. Salinization severely restricts plant growth, which has a great impact on crop yields. According to statistics, the loss of crops in irrigated areas around the world due to salinization is as much as 11 billion US dollars, and it is increasing. At present, salinized soil accounts for about 30% of the total land area, and there are about 1 billion ha in the world. 2 The land is in a state of salinization. According to the statistical report of the second national census organized by the Ministry of Agriculture, the total area of ​​saline soil in China accounts for 4.88% of the available land area in the country, of which the salinize...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P21/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 井大炜马海林刘方春刘幸红马丙尧杜振宇杨庆山周健彭琳
Owner SHANDONG FOREST SCI RES INST
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