Free fatty acid detection kit and preparation method thereof

A technology for detecting kits and free fatty acids, which is applied in the field of medical testing, can solve the problems of not essentially improving the sensitivity of kits, poor stability of kits, short storage time, etc., achieve good self-sterilizing ability, improve detection sensitivity, sensitivity Improved effect

Inactive Publication Date: 2018-06-01
李宏奎
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The report uses two non-ionic surfactants "alkyl glycoside APG0810 and lauryl polyoxyethylene ether" in combination with EDTA-2Na and mannitol to stabilize 4-aminoantipyrine and acyl coenzyme A activity of oxidase, peroxidase, etc., so as to solve the problem of poor stability and short storage time of the existing single stabilizer kit
However, since the sensitivity of the kit is closely related to the enzyme activity, the kit disclosed in the above report only prolongs the storage time on the basis of ensuring the original activity of the enzyme, and does not substantially improve the sensitivity of the kit.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Free fatty acid detection kit and preparation method thereof
  • Free fatty acid detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A free fatty acid detection kit, comprising reagents A and B, wherein:

[0045] Described reagent A component is as follows:

[0046] Sodium dihydrogen phosphate 100-120mmol / L

[0047] Coenzyme A 0.09-0.12mmol / L

[0048] Adenosine triphosphate 5-6.5mmol / L

[0049] Acyl-CoA synthetase 0.8-1.0ku / L

[0050] MgCl 2 3.5-4.2mmol / L

[0051] Trinder substrate 1.0-1.2g / L

[0052] Appropriate amount of magnetized water

[0053] Adjust the pH to 7.2;

[0054] Described reagent B component is as follows:

[0055] Sodium dihydrogen phosphate 120-155mmol / L

[0056] 4-Aminoantipyrine 28-35mmol / L

[0057] Acyl-CoA oxidase 78-95ku / L

[0058] Peroxidase 80-92KU / L

[0059] Flavin adenine dinucleotide 4.2-4.9mmol / l

[0060] EDTA-2Na 2-2.4g / L

[0061]Sodium N-cocoyl glutamate 3.0-4.2g / L

[0062] Fatty alcohol polyoxyethylene sodium sulfate 1.5-2.9g / L

[0063] N-acyl-L-serine sodium 2.4-3.5g / L

[0064] Mannitol 1-1.5g / L

[0065] Sodium citrate 24-35mmol / L

[0066] Appropr...

Embodiment 2

[0075] Control group A1: Compared with Example 1, the magnetized water in reagents A and B was replaced with double distilled water, and the remaining components and dosages were exactly the same;

[0076] Control group A2: Comparative Example 1, remove the EDTA-2Na in the reagent B, the reduced amount is made up with water, and all the other operations are the same as in Example 1;

[0077] Control group A3: Compared with Example 1, the mannitol in the reagent B was removed, and the reduced amount was made up with water, and the rest of the operations were the same as in Example 1;

[0078] Control group A4: comparative example 1, removes the sodium N-cocoyl glutamate in the reagent B, the reduced amount is filled up with water, and all the other operations are identical with embodiment 1;

[0079] Control group A5: Comparative Example 1, remove the fatty alcohol polyoxyethylene sodium sulfate in the reagent B, make up the reduced amount with water, and all the other operatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a free fatty acid detection kit and a preparation method thereof. The kit comprises reagents A and B, wherein the reagent A is prepared from the following components: sodium dihydrogen phosphate, a coenzyme A, triphosadenine, acyl coenzyme A synthetase, MgCl2, Trinder substrate and a proper amount of magnetized water, and the pH value is regulated to 7.2. The reagent B is prepared from the following components: sodium dihydrogen phosphate, 4-aminoantipyrine, acyl coenzyme A oxidase, peroxidase, flavin adenine dinucleotide, EDTA-2Na, N-sodium cocoanutylglutamate, sodiumlauryl ether sulphate, sodium N-acyl-L-serine, mannitol, sodium citrate and a proper amount of magnetized water, and the pH value is regulated to 7.2. The kit has the advantages of high and stable enzymatic activity and long preservation life; after the kit is preserved for about 15 days at 35-37 DEG C, the deviation of the detection result is only 1.99%; and the sensitivity is improved by 3.45%.

Description

technical field [0001] The invention belongs to the technical field of medical detection, in particular to a free fatty acid detection kit and a preparation method thereof. Background technique [0002] Free fatty acids, that is, non-esterified fatty acids, are intermediate products of fat metabolism in the human body, substrates of cell membrane lipid structures, and donors of intracellular signaling molecules such as prostaglandins. When glycogen, the energy source for muscle activity, is depleted, adipose tissue will decompose neutral fat into free fatty acids for energy use. Although free fatty acids only account for a small part of body fat, they meet a large part of energy demand, so they are important indicators for monitoring body fat metabolism and glucose metabolism. Free fatty acids can not only reflect the body's fat metabolism and blood lipid levels, evaluate blood sugar and assist in the diagnosis of diabetes, but also reflect a variety of other pathological a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/28
CPCC12Q1/28C12Q1/48G01N2333/91057
Inventor 李宏奎
Owner 李宏奎
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap