3-Phenoxy benzoic acid degrading bacterium and application thereof

A technology of phenoxybenzoic acid and degrading bacteria, applied in the biological field, can solve the problems of no degradation, pesticide residues, incomplete mineralization and degradation of 3-phenoxybenzoic acid, etc.

Inactive Publication Date: 2018-06-12
SICHUAN UNIV
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Problems solved by technology

However, there are few types of bacterial strains that can degrade 3-phenoxybenzoic acid screened at home and abroad, and some strains cannot completely mineralize and degrade 3-phenoxybenzoic acid, and there are still pesticide residues.
In addition, in the prior art, it has been found that Sphingomonas has a patent of degradation ability (application number: 201510775953.X, application publication number: CN 105274031A, application publication date: January 27, 2016), and the obtained sphingosine Sphingomonas sp. can be used to enhance the degradation of residual chlorpyrifos in leeks, but no Sphingomonas can degrade 3-phenoxybenzoic acid

Method used

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  • 3-Phenoxy benzoic acid degrading bacterium and application thereof

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Embodiment Construction

[0011] The following examples illustrate the present invention, but the present invention is not limited to the following examples.

[0012] 1. Strain screening method

[0013] (1) Basal salt medium (MM) prepared ammonium sulfate 1.5g, potassium dihydrogen phosphate 0.5g, dipotassium hydrogen phosphate 1.5g, sodium chloride 0.5g, magnesium sulfate 4 0.2g, Tween-80 2g, deionized Water 1000mL, pH 7.5, sterilized at 121°C for 15min.

[0014] (2) Bacterial sample collection About 200g of sludge sample was taken from the sewage outlet of the pesticide production plant.

[0015] (3) Acclimatization 5.0 g of bacterial samples were weighed, placed in 30 mL of MM medium with a concentration of 100 μg / mL of 3-phenoxybenzoic acid, and cultured at 30°C and 180 r / min for 7 days with shaking. Then transfer the culture solution to 30 mL of MM medium with a concentration of 200 μg / mL of 3-phenoxybenzoic acid according to the inoculum amount of 5.0% (v / v), culture at 30° C. and shake at 180 ...

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Abstract

The invention discloses 3-phenoxy benzoic acid degrading bacterium which is Sphingomonas sp. SC-1, and GenBank login number for its 16S rDNA is JN857975. The 3-phenoxy benzoic acid degrading bacteriumis originated from pesticide manufacturer sludge and is obtained by 3-phenoxy benzoic acid concentration gradient domestication, primary screening, separating and screening, and secondary screening.A colony of the 3-phenoxy benzoic acid degrading bacterium is colorless and transaprent and has neat edge and moist surface; individuals of the 3-phenoxy benzoic acid degrading bacterium are rod shaped and gram negative, having no spore; both glucose and glycerol ferments show positiveness, and both acid and gas are produced; no gel or starch is hydrolyzed; the growth of the bacterium requires sodium chloride and potassium chloride; phenylalanine dehydrogenase is positive; the growth requires oxygen. The 3-phenoxy benzoic acid degrading bacterium can grow with 3-phenoxy benzoic acid as the unique carbon source and energy and can degrade 3-phenoxy benzoic acid having a concentration of 300 mu g / mL in a medium to the rate of 100%.

Description

technical field [0001] The invention provides a 3-phenoxybenzoic acid degrading bacterium and application thereof, belonging to the field of biotechnology. Background technique [0002] Pyrethroid pesticides are a class of highly efficient, broad-spectrum, and neurotoxic insecticides that are artificially synthesized. They are currently widely used in agriculture, household hygiene pest control, and food storage. The degradation of most of these pesticides will produce an intermediate degradation product - 3-phenoxybenzoic acid, which is relatively weak in hydrophobicity, stronger in toxicity and mobility than its parent pesticide, easy to accumulate in the environment, and has a half-life of up to 180 days. With the extensive use of pyrethroid pesticides, its degradation intermediate product, 3-phenoxybenzoic acid, not only causes secondary pollution of agricultural products, but also hinders the biomineralization of pyrethroid pesticides, thus blocking the biomineralizatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C12N1/205C12R2001/01
Inventor 姚开黄雨婷贾冬英赵甲元迟原龙
Owner SICHUAN UNIV
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