Bacillus subtilis, compound microbial agent, organic matrix and preparation and application thereof

A technology of compound microbial agent and Bacillus subtilis, which is applied in the preparation of organic fertilizers, treatment of bio-organic parts, microorganisms, etc., can solve the problem of ineffective composting by microorganisms, and achieve shortened compost maturity time and strong tolerance , Increase the effect of water retention

Active Publication Date: 2018-06-12
上海成土养木环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It aims to solve the problem of insignificant composting effect initiated by

Method used

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  • Bacillus subtilis, compound microbial agent, organic matrix and preparation and application thereof
  • Bacillus subtilis, compound microbial agent, organic matrix and preparation and application thereof
  • Bacillus subtilis, compound microbial agent, organic matrix and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1 Acquisition, Identification and Preservation of Bacillus subtilis

[0071] The present invention obtains a strain of Bacillus subtilis BS-W1 from livestock and poultry feces high-temperature composting raw materials. The strain has been preserved in the China Center for Type Culture Collection on October 19, 2017, and the preservation number is CCTCC M 2017589.

[0072] The bacillus subtilis isolated in the invention can withstand high temperature composting environment (70°C). And it can secrete a variety of enzymes to efficiently degrade compost materials such as manure and straw.

[0073] After morphological identification and 16S rRNA, ITS-2 and rbcL amplified sequence developmental tree analysis, it was determined that the strain was Bacillus subtilis (according to the international naming rules: genus name + species name + strain name to name the strain, genus name, The species name and strain name are respectively Bacillus, subtilis and BS-W1), named B...

Embodiment 2

[0074] Example 2 Composite microbial bacterial agent for high-temperature aerobic composting

[0075] The Bacillus subtilis BS-W1 of the invention can be used for high-temperature aerobic composting. Generally, it is formulated with other microorganisms into a composite microbial agent for high-temperature aerobic composting.

[0076] The range of parts by weight between the components in the composite microbial agent 1 is: 1 part by weight of Bacillus subtilis, 5 parts by weight of Aspergillus niger, 1 part by weight of Bacillus amyloliquefaciens, 5 parts by weight of Candida tropicalis, 1 part by weight of Trichoderma, 3 parts by weight of Streptomyces grisea, 1 part by weight of Phanerochaete chrysosporium, 3 parts by weight of Pseudomonas fluorescens, 1 part by weight of Lactobacillus brevis, and 3 parts by weight of Bacillus longum. .

[0077] The range of parts by weight between the components in the composite microbial agent 2 is: 5 parts by weight of Bacillus subtili...

Embodiment 3

[0087] Example 3 Preparation of organic matrix using cattle waste and apple pomace

[0088] Organic matrix formula in the present embodiment:

[0089] The parts by weight of each raw material are: 30 parts by weight of waste cattle breeding fermented bedding material, 30 parts by weight of cow solid manure, 20 parts by weight of apple pomace, 8 parts by weight of biochar, 6 parts by weight of rice bran, 3 parts by weight of peat, pearl 3 parts by weight of rock, and 1 part by weight of composite microbial bacterial agent is added to ferment. The composition and parts by weight of the composite microbial bacterial agent in the present embodiment are: 3 parts of Bacillus subtilis, 5 parts of Aspergillus niger, 3 parts of Bacillus amyloliquefaciens, 3 parts of Candida tropicalis, 3 parts of Trichoderma reesei, 1 part of Streptomyces rubrum, 3 parts of Phanerochaete chrysosporium, 1 part of Pseudomonas fluorescens, 1 part of Lactobacillus brevis, and 1 part of Bacillus longum.

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Abstract

The invention belongs to the field of organic matrixes and particularly relates to bacillus subtilis, compound microbial agent, organic matrix and preparation and application thereof. A preservation number of Bacillus subtilis BS-W1 is CCTCC M 2017589. The bacillus subtilis can be prepared into the compound microbial agent to further obtain the organic matrix by preparation through high-temperature composting. Since bacillus subtilis strains have high tolerance capacity under the fermentation condition of 70 DEG C and are capable of secreting various hydrolysis enzymes to efficiently degrade macromolecular organics such as proteins, starch and cellulose in raw materials, and the compost center temperature can be increased to 70 DEG C. The composting fermentation temperature is increased ascompared with that of a conventional composting technique, and accordingly contents of organics and total nutrients are increased remarkably, and pathogenic bacteria, parasite ova, weed seeds and thelike can be effectively killed.

Description

technical field [0001] The invention belongs to the field of organic substrates, and in particular relates to a bacillus subtilis, a composite microbial agent, an organic substrate and its preparation and application. Background technique [0002] Organic substrate cultivation refers to the use of organic matter such as livestock and poultry manure, crop straw, mushroom residue, peat, sawdust, etc., after high-temperature fermentation treatment, mixed in a certain proportion to form a relatively stable and buffering full-nutrient cultivation substrate raw material. The cultivation substrate made entirely of organic matter has poor air permeability and water retention. Therefore, in order to improve the physical and chemical properties of the cultivation substrate, vermiculite, perlite, river sand, cinder and other inorganic substances can be mixed with it in a certain proportion to form an organic-inorganic substrate. Model cultivation substrate. [0003] At present, the so...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/08C05G3/04C05F17/00C12R1/125C12R1/685C12R1/07C12R1/74C12R1/885C12R1/465C12R1/645C12R1/39C12R1/24C05G3/80
CPCC12N1/14C12N1/16C12N1/20C05D9/00C05F11/08C05F17/00C05G3/80C12R2001/125C12N1/205C05F3/00C05F5/002C05F9/04C05F11/02Y02W30/40
Inventor 张久琴张文高潘家庆
Owner 上海成土养木环保科技有限公司
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