Construction method for obtaining aseptic seedling of lotus flower

Abstract

The invention relates to a construction method for obtaining an aseptic seedling of a lotus flower, and belongs to the technical field of plant tissue culture. The construction method comprises the following steps of (1), sterilization: selecting a round and full lotus seed of a Guangchang white lotus root, and disinfecting by using 75 percent alcohol and 3 percent sodium hypochlorite on an aseptic operating platform for later use; (2), hull breakage and soaking: breaking a hull by using pruning shears, leveling and soaking the seed of the lotus flower, whose hull is already broken, in asepticwater for 7h or above, wherein the seed absorbs water and expands, and a seed husk is separated from a seed leaf; (3), stripping of the seed husk: stripping a seed coat outside the seed, soaking theseed without the seed husk in the 75 percent alcohol for 2min for disinfection, taking the seed out, and air-drying; (4): obtaining of a seed embryo: manually pinching open the seed, which already absorbs the water and expands, after moisture on the surface of the seed is evaporated, and taking the seed embryo down by using tweezers; (5), primary selection and inoculation: carrying out the primaryselection on the embryo, and inoculating the embryo which is qualified in the primary selection in an MS (Murashige-Skoog) culture medium. The construction method is used for obviously improving theutilization rate and survival rate of the lotus seed, and moreover, the contamination rate and the loss rate are furthest decreased.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a construction method for obtaining sterile lotus seedlings. Background technique [0002] The lotus (Nelumbo nucifera Gaertn.) is a perennial aquatic flower of the genus Nelumbo of the family Nelumbo. It is one of the ten traditional famous flowers in China. It has high ornamental value, economic value and unique cultural connotation, and is deeply loved by people. [0003] With the development of physiology, biochemistry and other related disciplines, and with the increasing improvement of tissue culture technology, researchers often use biotechnology to create new germplasm, such as: combining genetic engineering to transfer disease resistance genes, drought resistance genes, etc. into cultivated varieties , to open up a new way to improve disease resistance and drought resistance; it can also be combined with polyploid induction to accelerate and enrich the polyplo...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a construction method for obtaining lotus sterile seedlings, so as to solve the problems of the prior art that the cultivation process is time-consuming, difficult to operate, extremely high loss rate, high pollution rate and low survival rate.