Construction method for obtaining aseptic seedling of lotus flower

A construction method and technology of sterile seedlings, applied in the field of construction of lotus sterile seedlings, can solve the problems of high loss rate, difficult operation, high pollution rate, etc., and achieve the effects of improving utilization rate, simplifying shell breaking, and avoiding pollution

Active Publication Date: 2018-06-29
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a construction method for obtaining lotus sterile seedlings, so as to solve the problems of t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A construction method of a lotus regeneration system is carried out in the following steps:

[0027] (1) Preparation of culture medium

[0028] Sterile seedling medium: MS+8g / L agar+30g / L sucrose

[0029] (2) Sterilization and shell breaking:

[0030] The round and plump lotus seeds of "Guangchang White Lotus" were sterilized with 75% alcohol for 30 seconds, rinsed once with sterile water, soaked in 3% sodium hypochlorite for 8 minutes, and rinsed with sterile water for 3 times. Use pruning shears on the opposite seed pedicle to break the shell. When breaking the shell, you must master the degree of breaking the shell, and it is better not to break the seed coat wrapped outside the cotyledon, because this can ensure that the water is filtered through the natural filtration of the thick cotyledon After being absorbed by the embryo, the aseptic environment can be guaranteed to the greatest extent; after soaking in sterile water for about 7 hours, the seeds can absorb wa...

Embodiment 2

[0035] A construction method of a lotus regeneration system is carried out in the following steps:

[0036] (1) Preparation of culture medium

[0037] Sterile seedling medium: MS+8g / L agar+30g / L sucrose

[0038] (2) Sterilization and shell breaking:

[0039] The round and plump lotus seeds of "Guangchang White Lotus" were sterilized with 75% alcohol for 30 seconds, rinsed once with sterile water, soaked in 3% sodium hypochlorite for 8 minutes, and rinsed with sterile water for 3 times. Use pruning shears on the opposite seed pedicle to break the shell. When breaking the shell, you must master the degree of breaking the shell, and it is better not to break the seed coat wrapped outside the cotyledon, because this can ensure that the water is filtered through the natural filtration of the thick cotyledon After being absorbed by the embryo, the aseptic environment is ensured to the greatest extent; soaked in sterile water for about 7 hours, the seeds can absorb water and become...

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Abstract

The invention relates to a construction method for obtaining an aseptic seedling of a lotus flower, and belongs to the technical field of plant tissue culture. The construction method comprises the following steps of (1), sterilization: selecting a round and full lotus seed of a Guangchang white lotus root, and disinfecting by using 75 percent alcohol and 3 percent sodium hypochlorite on an aseptic operating platform for later use; (2), hull breakage and soaking: breaking a hull by using pruning shears, leveling and soaking the seed of the lotus flower, whose hull is already broken, in asepticwater for 7h or above, wherein the seed absorbs water and expands, and a seed husk is separated from a seed leaf; (3), stripping of the seed husk: stripping a seed coat outside the seed, soaking theseed without the seed husk in the 75 percent alcohol for 2min for disinfection, taking the seed out, and air-drying; (4): obtaining of a seed embryo: manually pinching open the seed, which already absorbs the water and expands, after moisture on the surface of the seed is evaporated, and taking the seed embryo down by using tweezers; (5), primary selection and inoculation: carrying out the primaryselection on the embryo, and inoculating the embryo which is qualified in the primary selection in an MS (Murashige-Skoog) culture medium. The construction method is used for obviously improving theutilization rate and survival rate of the lotus seed, and moreover, the contamination rate and the loss rate are furthest decreased.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a construction method for obtaining sterile lotus seedlings. Background technique [0002] The lotus (Nelumbo nucifera Gaertn.) is a perennial aquatic flower of the genus Nelumbo of the family Nelumbo. It is one of the ten traditional famous flowers in China. It has high ornamental value, economic value and unique cultural connotation, and is deeply loved by people. [0003] With the development of physiology, biochemistry and other related disciplines, and with the increasing improvement of tissue culture technology, researchers often use biotechnology to create new germplasm, such as: combining genetic engineering to transfer disease resistance genes, drought resistance genes, etc. into cultivated varieties , to open up a new way to improve disease resistance and drought resistance; it can also be combined with polyploid induction to accelerate and enrich the polyplo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 徐迎春王华金奇江王彦杰
Owner NANJING AGRICULTURAL UNIVERSITY
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