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Detection method of benzopyrene in microcapsule powder

A technology of microcapsule powder and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low extraction efficiency, low measurement accuracy, insufficient extraction, etc., and achieve the effect of improving the extraction rate

Inactive Publication Date: 2018-07-06
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for extracting benzopyrene in microcapsule powder in order to solve the problem of insufficient extraction of benzopyrene in microcapsule powder, low extraction efficiency and low measurement accuracy, and to provide a method for extracting benzopyrene in microcapsule powder. Detection method

Method used

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  • Detection method of benzopyrene in microcapsule powder
  • Detection method of benzopyrene in microcapsule powder
  • Detection method of benzopyrene in microcapsule powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Materials and instruments

[0029]Materials: methanol is LC-MS grade, purchased from Fisher Company of the United States; ethyl acetate and cyclohexane are HPLC grade, purchased from Fisher Company of the United States; benzo(a)pyrene standard substance; chromatographic column, Agilent ZORBAX SB-C18 ( 100mm×2.1mm, 3.5μm); polyunsaturated fatty acid microcapsule ARA and DHA powder, provided by Carbio Bioengineering (Wuhan) Co., Ltd.

[0030] Instruments: Agilent1260 high performance liquid chromatography, purchased from Agilent Corporation of the United States; QTRAP4500 mass spectrometer, purchased from Sciex Corporation of the United States; refrigerated centrifuge, purchased from Sigma Corporation of the United States; nitrogen blowing instrument, purchased from Shanghai Anpu Scientific Instrument Co., Ltd.); The ultrasonic cleaning machine was purchased from Ningbo Xinzhi Co., Ltd.

[0031] 2. Sample pretreatment

[0032] 2.1 Preparation of standard solution

...

Embodiment 2

[0051] 1, material and instrument: with embodiment 1.

[0052] 2. Sample pretreatment

[0053] 2.1 Preparation of standard solution: Same as Example 1.

[0054] 2.2 Extraction of samples

[0055] Weigh 1.0 g (accurate to 0.0001 g) of DHA microcapsule powder product in 3 parts each in a 40 mL plastic centrifuge tube, add 100 μL of benzo(a)pyrene standard solution (concentration 7.5 ng / mL) respectively, add 10 mL of n-hexane, vortex Spin for 60 s, sonicate for 5 min, centrifuge at 7000 r / min for 10 min, take the supernatant as the solution to be tested, and protect the sample from light during the whole process.

[0056] 2.3 Sample purification (refrigerated centrifugal purification)

[0057] Blow the sample to be purified to near dryness with nitrogen at 30°C, dilute to 1mL with methanol, put it into a 1mL centrifuge tube, centrifuge at -10°C, 12000r / min for 15min, take the supernatant, and put it into a 1.5mL injection vial , for analysis by liquid chromatography-tandem ma...

Embodiment 3

[0060] 1, material and instrument: with embodiment 1.

[0061] 2. Sample pretreatment

[0062] 2.1 Preparation of standard solution: Same as Example 1.

[0063] 2.2 Extraction of samples

[0064] Weigh each 3 parts of 1.0g sample (accurate to 0.0001g) of DHA microcapsule powder product in a 40mL plastic centrifuge tube, add 100 μL benzo(a)pyrene standard solution (concentration 7.5ng / mL) respectively, add 10mL n-hexane, Vortex for 30 s, sonicate for 10 min, centrifuge at 10,000 r / min for 5 min, take the supernatant as the solution to be tested, and protect the sample from light during the entire process.

[0065] 2.3 Sample purification (refrigerated centrifugal purification)

[0066] Blow the sample to be purified to near dryness with nitrogen at 30°C, dilute to 1mL with methanol, put it into a 1mL centrifuge tube, centrifuge at -10°C, 12000r / min for 20min, take the supernatant, and put it into a 1.5mL injection vial , for analysis by liquid chromatography-tandem mass spe...

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Abstract

The invention provides a detection method of benzopyrene in microcapsule powder. The detection method comprises a pretreatment step of a sample. The pretreatment step of the sample is as follows: adding a non-polar solvent into a microcapsule powder sample to obtain a mixed solution, oscillating the mixed solution in vortex for 30 to 60 seconds, performing ultrasonic extraction for 5 to 10 min, and performing centrifugal treatment at a rotating speed of 7,000 to 10,000 r / min for 5 to 10 min, wherein supernate is taken to be used as a solution to be detected, and the whole process is kept in adark place. The detection method of the benzopyrene in the microcapsule powder, which is provided by the invention, can quickly and fully extract the benzopyrene in the microcapsule powder; the extraction method is simple and efficient, can meet a detection requirement and is high in detection accuracy.

Description

technical field [0001] The invention relates to the technical field of microcapsule detection, in particular to a method for detecting benzopyrene in microcapsule powder. Background technique [0002] DHA (docosahexaenoic acid), commonly known as brain gold, is a very important unsaturated fatty acid for the human body and is an important member of the omega-3 unsaturated fatty acid family; ARA (eicosatetraenoic acid), Arachidonic acid, also known as arachidonic acid, belongs to the Omega6 family of long-chain polyunsaturated fatty acids. DHA and ARA are the main components of the development of human tissues and organs, the growth and maintenance of nervous system cells, especially the important fatty acid components in the brain, nerves, and visual cells, which play a very important role in the development of the brain and visual system of infants. It is an essential fatty acid in early childhood, which is very important for the intelligence and vision development of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 李翔宇汪志明舒敏陆姝欢肖敏刘宏荣
Owner CABIO BIOTECH WUHAN CO LTD
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