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Stem cell total extracted liquid composition for sensitive skin, preparation method and application thereof

A technology for skin stem cells and extracts, applied in the field of compositions containing stem cell components, can solve the problems of not being consistent with the application object, that is, the skin, difficult to fully absorb, unable to improve skin sensitivity performance, etc. Toxic and side effects, the effect of promoting repair

Inactive Publication Date: 2018-07-10
ZHEJIANG ORIGIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent CN107569441A discloses an anti-allergic whitening composition and its application, which contains rose stem cells and several plant extracts, indicating that it has an anti-allergic effect; but the composition is all plant-derived ingredients, which are not easily absorbed by human skin , and its main effect is whitening, at most it does not cause side effects of skin allergies, and it cannot improve the existing skin sensitivity
[0006] Chinese patent CN105055285A discloses a skin nutrient solution and its preparation method, which contains human neural stem cell conditioning culture solution, which can improve sensitive skin; but the source of the stem cells is not consistent with the application object, that is, the skin, and only the above-mentioned stem cells are used. Serum, which completely discards all the components contained in the stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of the whole extract of human skin stem cells:

[0026] Configure medium: basal medium is DMEM / F12, 25μg / L VEGF, 35μg / L EGF, 8μg / L NGF, 20mg / L human serum albumin, 25μg / L vitamin E, iron citrate 2mmol / L, grain Aminoamide 2mmol / L, gentamicin 50g / L, putrescine 1.6mg / L.

[0027] Culture human skin stem cells in this medium for 3 days, collect all the culture medium mixture containing stem cells, crush the stem cells in it with a cell crusher, so that the small molecular substances in the cells are completely released; centrifuge, and take the supernatant; The bottom precipitate was washed once with deionized water, centrifuged again, and the supernatant was taken; the two supernatants were combined, filtered through a 250nm pore-diameter filter membrane, and obtained.

Embodiment 2

[0028] Example 2 Preparation of stem cell whole extract composition

[0029] Add 500 parts of microelement penetration-promoting factor, 3 parts of vitamin E, 2 parts of allantoin, and 2 parts of hyaluronic acid into the whole extract of human skin stem cells (1000 parts) obtained in Example 1, mix well, and freeze at 4°C Store in box.

[0030] Unless otherwise specified, the parts in the present invention are all in parts by mass.

Embodiment 3

[0031] Example 3 Preparation of stem cell whole extract composition (proportioning scheme 2)

[0032] Add 100 parts of microelement penetration enhancing factor, 1 part of vitamin E, 1 part of allantoin, and 1 part of hyaluronic acid into the whole extract of human skin stem cells obtained in Example 1 (800 parts), mix well, and freeze at 4 °C Store in box.

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PUM

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Abstract

The invention relates to a stem cell total extracted liquid composition for sensitive skin. The composition comprises a human skin stem cell total extracted liquid and a microelement permeation enhancing factor, and also can contain one or more of a humectant, a pH regulator, a viscosity regulator, an antioxidant, an emulsifier, a soothing component and the like, but is free of alcohol, essence, or preservative. The composition has no irritation to skin, also can be fully absorbed by skin, and more effectively improves the sensitive state of the skin due to substantial increase of active ingredients.

Description

technical field [0001] The invention belongs to the field of beauty and cosmetics, and mainly relates to a composition for sensitive skin, especially a composition containing stem cell components for sensitive skin. Background technique [0002] Sensitive skin is a type of problem skin that can be found in any skin type. It is generally believed that the pathological changes of sensitive skin are due to the damage of skin cells, which reduces the skin's immunity, and the thinning of the stratum corneum leads to insufficient moisture of the skin, which eventually leads to the skin's barrier function being too weak to resist external stimuli; then the nerve fibers of the skin Because it is often stimulated by the outside world, it is too excited, which is prone to redness, fever, itching, tingling, rash and other discomfort phenomena. [0003] Sensitive skin generally looks thinner, prone to allergies, and red blood streaks on the face are obvious; the skin is prone to rednes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/67A61K8/49A61K8/73A61Q19/00A61P37/08C12N5/071
CPCA61K8/99A61K8/4946A61K8/678A61K8/735A61Q19/005C12N5/0625C12N2500/30C12N2500/32C12N2500/38C12N2500/46C12N2501/11C12N2501/165C12N2501/195
Inventor 宣丹英江克光谭德松何祖法
Owner ZHEJIANG ORIGIN BIOTECH
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