Inactivated probiotic bacillus injection

A technology of Bacillus and Bacillus subtilis, applied in the field of antibacterial and antiviral injections

Active Publication Date: 2018-07-27
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above research, we speculate that the inactivation of probiotic bacillus into injections should also have antibacterial and antiviral effects on animal injections. After reviewing literature and patent databases, we found that there is no such inactivation of probiotic bac...

Method used

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  • Inactivated probiotic bacillus injection
  • Inactivated probiotic bacillus injection
  • Inactivated probiotic bacillus injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Inoculate Bacillus subtilis (Latin name: Bacillus subtilis, purchased from China Industrial Microorganism Culture Collection Management Center, preservation number: CICC 10012) into the medium (weigh 10g peptone, 10g sodium chloride, 5g yeast powder, add water to 1000mL , adjusted the pH to 7.0, sterilized at 121°C for 20 minutes), cultured in a constant temperature shaker at 37°C at a speed of 140r / min for 18-24 hours, then centrifuged at 3000 rpm for 5 minutes, removed the supernatant culture solution and retained the precipitate, added sterile Wash the precipitate with normal saline, centrifuge for 5 minutes, repeat the washing 3 times, add sterile normal saline, mix with the precipitate, and make a suspension. Take a certain amount of Bacillus subtilis physiological saline suspension, and count the bacteria on a THOMA bacterial counting plate, so that each ml suspension contains 1×10 9 Bacillus subtilis bacteria. The prepared Bacillus subtilis physiological saline ...

Embodiment 2

[0022] Inoculate Bacillus licheniformis (Bacillus licheniformis, Latin name: Bacillus licheniformis, purchased from China Industrial Microorganism Culture Collection Management Center, preservation number: CICC24236) in the medium (weigh 10g peptone, 10g sodium chloride, 5g yeast powder, Add water to 1000mL, adjust the pH to 7.0, sterilize at 121°C for 20min to prepare), incubate in a constant temperature shaker at 37°C at a speed of 140r / min for 18-24 hours, then centrifuge at 3000rpm for 5 minutes, remove the upper culture solution and retain the precipitate, Add sterile normal saline to wash the precipitate, centrifuge for 5 minutes, repeat the washing 3 times, add sterile normal saline, mix with the precipitate, and make a suspension. Take a certain amount of Bacillus licheniformis saline suspension, and count the bacteria on the THOMA bacterial counting plate, so that each ml suspension contains 1×10 9 Bacillus licheniformis. The prepared Bacillus licheniformis saline su...

Embodiment 3

[0024] The prevention and control effects of the inactivated Bacillus subtilis injection prepared in Example 1 and the inactivated Bacillus licheniformis injection prepared in Example 2 on Salmonella-infected mice were tested. Divide clean-grade Kunming mice with a body weight of 18-22g into 3 groups, namely the normal control group, the Salmonella group, the inactivated Bacillus subtilis injection group and the inactivated Bacillus licheniformis injection group, with 30 mice in each group, male and female Half and half. The mice in the normal control group and the Salmonella group were injected with sterile normal saline in the tail vein, and the inactivated Bacillus subtilis injection group and the inactivated Bacillus licheniformis injection group were respectively injected with corresponding drugs with a volume of 0.1 mL / 10 g. 24 hours after administration once, the mice in Salmonella group, inactivated Bacillus subtilis injection group and inactivated Bacillus licheniform...

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Abstract

The invention relates to an antibacterial and anti-virus injection and belongs to the field of biomedicines. A main component of the medicine is inactivated probiotic bacillus which is obtained by inactivating living probiotic bacillus. Inactivated probiotic bacillus intramuscular administration has antibacterial and anti-virus effects, can be used for prevention, treatment or adjuvant treatment of various bacterial diseases and virus diseases of human or animals and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to an antibacterial and antiviral injection. Background technique [0002] Probiotic Bacillus is a class of aerobic or facultatively anaerobic, spore-forming rod-shaped bacteria that have beneficial properties to the body and are Gram-positive. Probiotic bacillus includes: Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Bacillus thuringiensis, Bacillus amyloliquefaciens, Bacillus coagulans and aerobic Bacillus etc. Because this type of bacillus has good probiotic properties such as protecting the digestive tract and promoting feed conversion, it is widely used in human medicine, livestock and poultry, and aquaculture. At present, there are also people who make injections of live anaerobic bacillus and directly inject them into the inside of cancerous solid tumors to treat cancer (patent publication number: CN106474161A). [0003] In the past, we found that the inactivated la...

Claims

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Application Information

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IPC IPC(8): A61K35/742A61K9/10A61P31/04A61P31/12
CPCA61K9/0019A61K9/10A61K35/742A61K2300/00
Inventor 石有斐张勇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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