Novel polypeptide for tumor targeting and application thereof

A tumor-targeting, new type of technology, applied in the field of biomedicine, can solve the problems of tumor treatment impact, reduce mortality, large drug dosage, etc., and achieve the effect of high activity, high permeability and low cost

Active Publication Date: 2018-07-31
中国医科大学
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  • Abstract
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  • Application Information

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Problems solved by technology

However, an ideal method to screen for ovarian cancer and reduce its mortality has not been found so far
At present, the basic treatment method is surgery supplemented by chemotherapy. The existing anticancer drugs mainly include metal anticancer drugs, anticancer active ingredients extracted from natural products, targeted preparations of anticancer drugs, genetic engineering drugs, and nano controlled release anticancer drugs. Drugs and other five categories, these drugs usually have the disadvantages of relatively large toxic and side effects, large drug dosage, and prone to acquired drug resistance, which affect the treatment of tumors

Method used

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  • Novel polypeptide for tumor targeting and application thereof
  • Novel polypeptide for tumor targeting and application thereof
  • Novel polypeptide for tumor targeting and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Experimental materials

[0022] 1.1 Phage peptide library, cells and host bacteria.

[0023] Phage 12 peptide library, Escherichia coli E.coli ER2738, human ovarian epithelial cells HOSEpiC, human ovarian adenocarcinoma SK-OV-3, tumor-bearing mice.

[0024] 1.2 Experimental reagents

[0025] McCoy's 5A medium, RPMI-1640 medium, trypsin, FITG-labeled rabbit anti-mouse, fetal bovine serum, yeast powder, peptone, agar powder, tetracycline stock solution, Tween-20 (tween-20), bovine serum albumin BSA , HRP-anti-M13 filamentous phage antibody, M13 phage single-stranded DNA extraction kit, IPTG, X-gal, PEG-8000, TMB.

[0026] 1.3 Experimental working solution

[0027] 1×PBS, LB liquid medium, LB-Tet solid plate, top agar, IPTG / X-gal working solution, IPTG / X-gal plate, PEG-NaCl, TBS buffer, 0.1% TBST, 0.5% TBST, 4 %paraformaldehyde fixative, preparation of TBS-NaN3 solution, 3% BSA blocking solution, sodium iodide buffer, TE buffer, TMB working solution, tetracycline st...

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Abstract

The invention belongs to the field of biomedicine, and particularly relates to a novel polypeptide with a targeting effect on human ovarian cancer cells and application thereof. The amino acid sequence of the polypeptide is WNPLLLTRLLPA(SEQ ID No.1), and in the polypeptide molecule, through deletion, insertion or displacement of one or several amino acids, a polypeptide derivative with the same biological function as the polypeptide molecule is obtained. The polypeptide has the advantages of small molecular mass, high permeability, high specificity, low costs and the like, and shows unique advantages in tumor targeting diagnosis and treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a novel polypeptide having a targeting effect on human ovarian cancer cells and its application. Background technique [0002] Ovarian cancer is one of the most common female malignancies, its incidence rate ranks second among gynecological malignancies, and its mortality rate ranks first among gynecological malignancies. Because the ovary is located in the deep part of the pelvis, the onset is relatively hidden, it is difficult to detect the disease in the early stage and the disease develops rapidly, and there is a lack of effective screening and early diagnosis methods. More than 70% of the patients are already in the advanced stage when they see a doctor. At present, the 5-year survival rate of patients with advanced ovarian cancer is only 20%-40%, while that of early-stage patients can reach 70%-90%. Therefore, both early diagnosis and treatment of ovarian cancer are cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06G01N33/574G01N33/68A61K47/42A61P35/00
CPCA61K47/42C07K7/06G01N33/574G01N33/68A61K9/0019A61K38/00A61P35/00C07K7/08
Inventor 魏敏杰赵琳卫倩
Owner 中国医科大学
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