Novel polypeptide for tumor targeting and application thereof
A tumor-targeting, new type of technology, applied in the field of biomedicine, can solve the problems of tumor treatment impact, reduce mortality, large drug dosage, etc., and achieve the effect of high activity, high permeability and low cost
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[0021] 1. Experimental materials
[0022] 1.1 Phage peptide library, cells and host bacteria.
[0023] Phage 12 peptide library, Escherichia coli E.coli ER2738, human ovarian epithelial cells HOSEpiC, human ovarian adenocarcinoma SK-OV-3, tumor-bearing mice.
[0024] 1.2 Experimental reagents
[0025] McCoy's 5A medium, RPMI-1640 medium, trypsin, FITG-labeled rabbit anti-mouse, fetal bovine serum, yeast powder, peptone, agar powder, tetracycline stock solution, Tween-20 (tween-20), bovine serum albumin BSA , HRP-anti-M13 filamentous phage antibody, M13 phage single-stranded DNA extraction kit, IPTG, X-gal, PEG-8000, TMB.
[0026] 1.3 Experimental working solution
[0027] 1×PBS, LB liquid medium, LB-Tet solid plate, top agar, IPTG / X-gal working solution, IPTG / X-gal plate, PEG-NaCl, TBS buffer, 0.1% TBST, 0.5% TBST, 4 %paraformaldehyde fixative, preparation of TBS-NaN3 solution, 3% BSA blocking solution, sodium iodide buffer, TE buffer, TMB working solution, tetracycline st...
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