Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing high-yield tremella polysaccharide by fementing tremella spores

A technology of tremella polysaccharides and tremella spores, which is applied in the field of preparation of high-yield tremella polysaccharides by fermentation of tremella spores, can solve the problems of low yield, high cost, and complicated production methods of tremella polysaccharides, and achieve high-yield extracellular polysaccharides, convenient operation, and easy realization The effect of industrialization

Inactive Publication Date: 2018-07-31
NINGDE NORMAL UNIV
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a high-yield tremella polysaccharide preparation method for tremella spore fermentation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0019] Specific embodiment one: what this embodiment records is a kind of preparation method of tremella spore fermentation high-yield tremella polysaccharide, described method step is as follows:

[0020] Step 1: Purchasing Tremella fungus strain Tr21 from Xinghua Fungi Research Institute, Gutian County, Fujian Province;

[0021] Step 2: Prepare slant preservation medium: take 200 g of peeled potatoes and cut them into small pieces, add 800 mL of distilled water, boil for 20-30 min, filter with gauze to obtain the filtrate, add 20 g of glucose, 1 g of peptone, and 1 g of KH to the filtrate 2 PO 4 , 0.5 g MgSO 4 ·7H 2 0. 20 g of agar, after heating and dissolving, add water to a total volume of 1 L, and the pH is natural, and sterilize at 115° C. for 20 to 30 min; the effect of the slant preservation medium is to increase the angular area;

[0022] Step 3: Activating Tremella fungus strains: inoculate the strain of Step 1 on the slant preservation medium of Step 2, and cult...

specific Embodiment approach 2

[0028] Embodiment 2: In Embodiment 1, a method for preparing Tremella spores fermented with high-yield Tremella polysaccharides, in step 5, the plant used in the plant extract is a plant of the genus Rhizoma Lauraceae.

Embodiment 1

[0030] (1) Activation of Tremella fungus strains: inoculate Tremella fungus mother species on the slant preservation medium composed of the following composition. Cut 200g of peeled potatoes into small pieces, add 800mL of distilled water, boil for 30min, filter with gauze to get the filtrate, add 20g of glucose, 1g of peptone, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, agar 2g, add water to 1L after heating to dissolve, pH is natural, sterilize at 115°C for 25min. After inoculation, culture at 24°C for 5 days.

[0031] (2) Cultivation of liquid seeds: add 20g glucose, 2g peptone, 1g yeast extract, 3 g K 2 HPO 4 , 1.5 g MgSO 4 ·7H 2 O, adjust the pH value to 5.8 with 1mol / L HCl or NaOH, and sterilize at 115°C for 30min. Under aseptic operation conditions, rinse with 10mL sterile normal saline to activate the growth of bacteria on the slant preservation medium to make a bacterial suspension, and add the bacterial suspension according to the inoculation amount of 6% of the volum...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing high-yield tremella polysaccharide by fermenting tremella spores and belongs to the technical field of microbial fermentation and culture. The method comprises the following steps: purchasing culture: namely a strain Tr21; placing an inclined surface preservation culture medium at 22.5 to 23.5 DEG C to perform cultivation for 4 to 8 days, and sterilizing a liquid seed culture medium; swinging to washing the spores on the inclined surface preservation culture medium by using 10 mL of normal saline, inoculating bacterial suspension according to theinoculation quantity which is 3 to 8 percent of the volume of the liquid seed culture medium, and performing shake cultivation for 2 to 4 days at the rotating speed of 170 to 200 r / min and at the cultivation temperature of 23 to 26 DEG C; sterilizing the liquid fermentation culture medium at 115 DEG C, inoculating, shaking uniformly and performing stationary culture for 4 to 8 hours in a 500-mL triangular bottle under the conditions that the liquid loading quantity is 100 mL and the inoculation quantity is 5 to 8 percent, and performing fermentation on a shaking table for 4 to 6 days at the culture temperature of 23 to 26 DEG C and at the stirring rotating speed of 180 to 220 r / min. The fermentation condition of the tremella polysaccharide is optimized, proliferation of the tremella spores in the fermentation liquid is promoted by adding a water leaching solution of lauraceae plant dried powder of 10 to 50 percent, the biomass is increased by 68.76 to 116.87 percent, and the yield ofexopolysaccharides is increased by 67.75 to 99.81 percent. The method is used for preparing the high-yield tremella polysaccharide by fermenting the tremella spores.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a preparation method for high-yield tremella polysaccharides fermented by tremella spores. Background technique [0002] Tremella ( Tremella fuciformis Berk), also known as white fungus, is a kind of higher colloidal fungus, which is a well-known nourishing nutrient and edible and medicinal fungus for health and longevity in my country. Modern pharmacological studies have shown that Tremella has physiological activities such as immune regulation, anti-tumor and aging, lowering blood sugar and blood lipids, anti-thrombosis and viruses, promoting nerve cell growth and preventing and treating cardiovascular diseases. The active substance is mainly tremella polysaccharide. Tremella polysaccharide has a variety of pharmacological and health functions, and is widely used in food, medicine and cosmetic industries. Tremella fungus has two forms of yeast spore...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/04C12N1/14C12R1/645
CPCC12N1/14C12P19/04
Inventor 阮少江邓湘凌王晓青罗佳敏刘颖林枫邱水玲赵丽刘春雷周逢芳
Owner NINGDE NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com