Acellular dermal matrix-guided tissue regeneration membrane material and its preparation method and application

A technology for guiding tissue regeneration and acellular dermis. It is used in tissue regeneration, medical science, prosthesis, etc. to achieve the effect of good mechanical barrier, providing space for cell growth, and convenient operation.

Active Publication Date: 2021-05-14
QINGDAO MARINE BIOPHARMACEUTICAL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention proposes an acellular dermal matrix guiding tissue regeneration membrane material and its preparation method and application, which solves the problem of carrying and spreading risks of zoonotic viruses in the guiding tissue regeneration membrane in the prior art

Method used

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  • Acellular dermal matrix-guided tissue regeneration membrane material and its preparation method and application
  • Acellular dermal matrix-guided tissue regeneration membrane material and its preparation method and application
  • Acellular dermal matrix-guided tissue regeneration membrane material and its preparation method and application

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preparation example Construction

[0032] A preparation method of the acellular dermal matrix-guided tissue regeneration membrane material of the present invention comprises the following steps:

[0033] 1) Pre-treatment: take fish skin, remove fish scales, inner fat and excess fish flesh;

[0034] 2) Washing: The fish skin obtained in step 1) is repeatedly rinsed several times with physiological saline, and then, repeatedly rinsed several times with ultrapure water;

[0035] 3) Remove the epidermis and prepare loose noodles: place the tissue obtained in step 1) in a 0.01-1.0mol / L acid solution, shake at 20-37°C for 5-30 minutes, to remove the epidermis and obtain loose noodles ;

[0036] 4) Stabilization: place the tissue obtained in step 3) in a 0.5-2.5 mol / L sodium chloride solution, and shake for 30-120 min at 20-37°C to stabilize the structure;

[0037] 5) Decellularization: freeze-thaw the tissue obtained in step 4) at -80 to -70°C, freeze and thaw several times, and then use 0.5 to 4.0% sodium deoxycho...

Embodiment 1

[0050] A preparation method of the acellular dermal matrix-guided tissue regeneration membrane material of the present invention comprises the following steps:

[0051] 1) Pre-treatment: remove the fish skin from the fish, and remove the fish scales, inner fat and excess fish meat with a knife;

[0052] 2) Washing: The fish skin obtained in step 1) was repeatedly washed with physiological saline for 3 times, each time lasting 10 minutes, and then repeatedly washed with ultrapure water for 3 times, each time lasting 10 minutes;

[0053] 3) Remove the epidermis and prepare loose noodles: put the material obtained in step 2) into 0.01mol / L formic acid solution, and shake for 30 minutes in a constant temperature shaker at 20°C to remove the epidermis and prepare loose noodles;

[0054] 4) Stabilization: put the material obtained in step 3) into a 0.5mol / L sodium chloride solution, and shake for 120 minutes in a constant temperature shaker at 20°C to stabilize the structure;

[00...

Embodiment 2

[0061] A preparation method of the acellular dermal matrix-guided tissue regeneration membrane material of the present invention comprises the following steps:

[0062] 1) Pre-treatment: remove the fish skin from the fish, and remove the fish scales, inner fat and excess fish meat with tweezers;

[0063] 2) Washing: the fish skin obtained in step 1) was repeatedly washed with physiological saline for 5 times, each time lasting 15 minutes, and then repeatedly washed with ultrapure water for 5 times, each time lasting 15 minutes;

[0064] 3) Remove the epidermis and prepare loose noodles: put the material obtained in step 2) into 0.1mol / L hydrochloric acid solution, shake for 10 minutes in a constant temperature shaker at 25°C, remove the epidermis and prepare loose noodles;

[0065] 4) Stabilization: put the material obtained in step 3) into a 1.0mol / L sodium chloride solution, and shake for 90 minutes in a constant temperature shaker at 37°C to stabilize the structure;

[006...

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Abstract

The invention provides an acellular dermal matrix-guided tissue regeneration membrane material and its preparation method and application, and relates to the technical field of medical biomaterials. The preparation method of the present invention comprises the following steps: pretreatment, removing the epidermis, preparing a loose surface, stabilizing, decellularizing, washing and sterilizing; the membrane material prepared by the method of the present invention uses fish skin as a raw material and has a three-dimensional space frame structure, including a dense surface composed of a biological basement membrane and a loose collagen surface composed of a dermal scaffold. The membrane material of the present invention avoids the risk of carrying and disseminating zoonotic viruses in the existing tissue repair materials, completely removes cell components, has low immunogenicity, increases the gap between collagen fibers, retains the structure of the dermis, and maintains tissue phase Good capacity, not only has a good mechanical barrier effect but also is conducive to the formation of new bone; when used in the repair of bone defects in oral implant surgery, the material can be absorbed, effectively avoiding the secondary operation and the damage to the patient.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to an acellular dermal matrix guided tissue regeneration membrane material and its preparation method and application. Background technique [0002] In recent years, with the continuous development and progress of the level of science and technology, the emergence of a new repair technology—membrane guided bone / tissue regeneration (MGBR / MGTR) has greatly improved the level of oral restorative science. . Guided bone / tissue regeneration (GBR / GTR) has the function of guiding osteogenesis and promoting the formation of osseointegration around the implant. Cells are faster than cells forming alveolar bone. Biomaterials are used to make a biofilm with a dense surface and a loose surface. The dense surface acts as a barrier to prevent fibroblasts and epithelial cells in soft tissues from growing into the bone defect area, avoiding these The cells compete with the cells capab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/58
CPCA61L27/3633A61L27/365A61L27/3687A61L27/3691A61L27/58A61L2430/02A61L2430/40
Inventor 王园园李八方
Owner QINGDAO MARINE BIOPHARMACEUTICAL RES INST
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