LRP4 antibody cell coated microporous plate, preparation method and human LRP4 antibody cell immunofluorescence detection kit

Abstract

Belonging to the technical field of immunodetection, the invention provides an LRP4 antibody cell coated microporous plate, a preparation method and a human LRP4 antibody cell immunofluorescence detection kit. The human LRP4 antibody cell immunofluorescence detection kit comprises the LRP4 cell coated microporous plate, a sample diluent, a positive reference substance, a secondary antibody and a blocking solution. The LRP4 antibody cell coated microporous plate provided by the invention is directly coated with LRP4-GFP-HEK293T recombinant cell and EGFP-HEK293T recombinant cell. Repetition of plasmid transfection, cell inoculation, cell culture and other processes within a short period of time can be avoided, and then the cost of every detection is reduced, and the detection time is shortened.

Description

technical field [0001] The invention belongs to the technical field of immune detection, and in particular relates to a LRP4 antibody cell-coated microwell plate, a preparation method and a human LRP4 antibody cell immunofluorescence detection kit. Background technique [0002] Myasthenia gravis (MG) is a neuromuscular junction transmission disorder mediated by autoantibodies; AChR antibodies account for about 80-90%, followed by MuSK antibodies. Studies in recent years have shown that low-density lipoprotein receptor-related protein 4 (LRP4) antibody is another antibody for MG, which may inhibit the formation of AChR clusters on the postsynaptic membrane by blocking the binding of agrin and MuSK, and then participate in the pathogenic process of MG . Clinically, clear serum antibody typing of MG patients is helpful for early diagnosis and precise treatment of the disease. [0003] The methods currently used to detect LRP4 antibodies include enzyme-linked immunosorbent ass...

AI Technical Summary

Problems solved by technology

The above method has the following problems: (1) The cost is high. At present, HEK293 cells expressing the LRP4 protein are obtained by direct plasmid transfection, which will lead to the unstable expression of the LRP4 protein in the recombinant cells (the plasmid will gradually lose its expression in the passage process). The purity of HEK293 cells expressing LRP4 protein is getting lower and lower during passage); the purity of recombinant cells obtained is not high; and when the purity is lower than a certain ratio, a series of steps such as plasmid transfection and cell inoculation need to be repeated, resulting in Increased costs

In addition, the current recombinant cell lines cannot be stored for a long time, and the process of cell culture and inoculation needs to be repeated for each test, resulting in increased costs

(2) It takes a long time. In view of the low purity and difficult preservation of recombinant cell lines obtained at present, a series of steps such as plasmid transfection and cell inoculation need to be repeated frequently, and the detection cycle is long

It is precisely because the operation of the above-mentioned detection method is relatively complicated, the required experimental conditions are relatively high, and the cycle is too long, which limits its application.

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