Rape pollen extract and application thereof
A rape pollen and extract technology is applied in the directions of drug combinations, plant raw materials, medical preparations containing active ingredients, etc., to achieve the effects of improving CAT activity and GSH content, increasing uric acid excretion, and reducing side effects
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Embodiment 1
[0032] The preparation of embodiment 1 extract
[0033] (1) Take the rapeseed pollen after the wall-breaking treatment, extract with 75% ethanol as solvent, and a solid-liquid ratio of 1:10 (m:V), filter and collect the extract, repeat the extraction of the filter residue twice, and combine the extract And concentrated under reduced pressure until no ethanol.
[0034] (2) extracting the extract with water-saturated n-butanol until the n-butanol layer is colorless, concentrating the n-butanol layer under reduced pressure and vacuum freeze-drying to obtain a crude rapeseed pollen extract;
[0035] (3) Dissolve the crude extract in water and put it on a polyamide column, rinse it with distilled water until the eluent is not turbid, then elute with 30%, 50% and 75% ethanol in turn, and combine the elutions of each concentration solution, concentrated under reduced pressure, vacuum freeze-dried, respectively marked as Fragment 1, Fragment 2 and Fragment 3; another crude extract wa...
Embodiment 2
[0036] Example 2 The inhibitory effect of the extract on XO
[0037] (1) Test method
[0038] Xanthine oxidase (XO) catalyzes xanthine (Xan) to produce uric acid and has a characteristic absorption peak at 290nm. The kinetics / time software of the ultraviolet spectrophotometer is used to measure the absorbance every 15s, and it is measured 20 times in total. The absorbance increases linearly with time, and its slope is the reaction rate of the enzyme. The larger the slope, the stronger the activity of the enzyme.
[0039] First, the five fragments prepared in Example 1 were dissolved in 1% DMSO to prepare sample solutions with different concentrations, and then 100 μL of 7.5×10 -8 mol / L XO solution and 50 μL sample solution were mixed, incubated at 37°C for 5 min, and then 100 μL of substrate solution Xan (5×10 -5 mol / L) to start the reaction, measure the change in absorbance at a wavelength of 290nm and calculate the slope R. Replace the sample with the same volume of 0.05...
Embodiment 3
[0044] Example 3 The evaluation of the uric acid-lowering effect of the extract
[0045] (1) Test method
[0046] 50 18-22g male Kunming mice were adapted to feeding for a week, and the animals were free to eat common feed and drinking water. After one week, the mice were randomly divided into 5 groups, which were blank group, model group, positive group, and rapeseed pollen crude extract 5 groups and 5 groups of fragments, 10 in each group. Carboxymethylcellulose sodium is mixed with distilled water to make a 0.5% solution, oxonate potassium is mixed with 0.5% CMC-Na and distilled water is mixed and stirred evenly, and the suspension of oxonate potassium is prepared, and the positive drug allopurinol is mixed with 0.5% CMC-Na and distilled water were mixed to prepare an allopurinol solution. Similarly, the crude extract and Fragment 5 used in the sample group were respectively mixed with 0.5% CMC-Na and distilled water to prepare corresponding suspensions.
[0047]All mice...
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