Method for preparing seaweed fertilizer by taking Enteromorpha as raw material

Abstract

The invention discloses a method for preparing a seaweed fertilizer by taking Enteromorpha as raw material. The method comprises the following steps: S1, cleaning the Enteromorpha, and performing airdrying and smashing to obtain seaweed particles; S2, dissolving the seaweed particles into a seaweed colloid solution; S3, performing enzymatic hydrolysis on the seaweed colloid solution to obtain a mixed solution; S4, centrifuging the mixed solution, collecting supernatant, adding chitosan into the supernatant, mixing and putting the mixture into a vacuum freeze dryer for dehydration to obtain seaweed powder; S5, preparing strain fermentation broth; S6, slowly adding the strain fermentation broth into the seaweed powder with continuous stirring, and stacking into a fermentation heap; S7, overturning the fermentation heap, re-stacking into a new fermentation heap, and spreading the fermentation heap to form the seaweed fertilizer. The Enteromorpha is treated through an enzymatic hydrolysismethod, so that effective components such as nutrient elements and phytohormones in the Enteromorpha can be kept to the maximum extent, little damage is caused to the effective components in the Enteromorpha, and the effect of the fertilizer at a later stage is not influenced.

Description

technical field

[0001] The invention relates to the technical field of seaweed fertilizer, in particular to a preparation method of seaweed fertilizer using enteromorpha as raw material. Background technique

[0002] The algal body is grass-green, tubular and membranous, clustered, with obvious main branches and slender branches, up to 1 meter high. The base is attached to the rock with a fixator, and grows on tidal flats and gravels in the mid-tidal zone. Due to global climate change, water eutrophication and other reasons, the green tide of the large marine alga Enteromorpha broke out. A large amount of Enteromorpha floats and gathers on the shore, blocking the waterway and destroying the marine ecosystem, seriously threatening the development of coastal fishery and tourism. Most types of marine products are widely distributed in oceans all over the world, and some species are found in brackish water or rivers. Also visible. It often grows on rocks or bogs in the intert...

Examples

Embodiment 1

[0025] A kind of seaweed fertilizer preparation method using Enteromorpha as raw material comprises the following steps:

[0026] S1. Clean the fresh Enteromorpha with tap water 3 times, soak the cleaned Enteromorpha in 95% ethanol for 40 h, take out the Enteromorpha and wash it with distilled water 3 times, air-dry the Enteromorpha under natural conditions, and air-dry the Enteromorpha enteromorpha is pulverized using a colloid mill to obtain seaweed particles with a particle size of 300 meshes;

[0027] S2. Dissolving the seaweed particles with distilled water, stirring continuously during the dissolving process, and keeping the water temperature at 37°C during dissolution, to obtain a seaweed colloid solution;

[0028] S3. Enzymolysis: Add cellulase with a final concentration of 1.5 g / L to the seaweed colloid solution, and mix the seaweed colloid solution continuously. After stirring for 4 hours, continue to add β-glucose with a final concentration of 1.8 g / L. Glycanase, g...

Embodiment 2

[0034] A kind of seaweed fertilizer preparation method using Enteromorpha as raw material comprises the following steps:

[0035] S1. Clean the fresh Enteromorpha with tap water for 3 times, soak the cleaned Enteromorpha in 95% ethanol for 42 h, take out the Enteromorpha and wash it with distilled water for 3 times, air-dry the Enteromorpha under natural conditions, and air-dry the Enteromorpha enteromorpha is pulverized using a colloid mill to obtain seaweed particles with a particle size of 300 meshes;

[0036] S2. Dissolving the seaweed particles with distilled water, stirring continuously during the dissolving process, and keeping the water temperature at 37°C during dissolution, to obtain a seaweed colloid solution;

[0037] S3. Enzymatic hydrolysis: Add cellulase with a final concentration of 1.8 g / L to the seaweed colloid solution, stir and mix the seaweed colloid solution continuously, after stirring for 5 hours, continue to add β-glucose with a final concentration of ...

Embodiment 3

[0043] A kind of seaweed fertilizer preparation method using Enteromorpha as raw material comprises the following steps:

[0044] S1. Clean the fresh Enteromorpha with tap water for 3 times, soak the cleaned Enteromorpha in 95% ethanol for 42 h, take out the Enteromorpha and wash it with distilled water for 3 times, air-dry the Enteromorpha under natural conditions, and air-dry the Enteromorpha enteromorpha is pulverized using a colloid mill to obtain seaweed particles with a particle size of 260-300 mesh;

[0045] S2. Dissolving the seaweed particles with distilled water, stirring continuously during the dissolving process, and keeping the water temperature at 37°C during dissolution, to obtain a seaweed colloid solution;

[0046] S3. Enzymatic hydrolysis: Add cellulase with a final concentration of 2.0 g / L to the seaweed colloid solution, stir and mix the seaweed colloid solution continuously, after stirring for 6 hours, continue to add β-glucose enzyme with a final concentr...

technical field

[0001] The invention relates to the technical field of seaweed fertilizer, in particular to a preparation method of seaweed fertilizer using enteromorpha as raw material. Background technique

[0002] The algal body is grass-green, tubular and membranous, clustered, with obvious main branches and slender branches, up to 1 meter high. The base is attached to the rock with a fixator, and grows on tidal flats and gravels in the mid-tidal zone. Due to global climate change, water eutrophication and other reasons, the green tide of the large marine alga Enteromorpha broke out. A large amount of Enteromorpha floats and gathers on the shore, blocking the waterway and destroying the marine ecosystem, seriously threatening the development of coastal fishery and tourism. Most types of marine products are widely distributed in oceans all over the world, and some species are found in brackish water or rivers. Also visible. It often grows on rocks or bogs in the intert...

Examples

Embodiment 1

[0025] A kind of seaweed fertilizer preparation method using Enteromorpha as raw material comprises the following steps:

[0026] S1. Clean the fresh Enteromorpha with tap water 3 times, soak the cleaned Enteromorpha in 95% ethanol for 40 h, take out the Enteromorpha and wash it with distilled water 3 times, air-dry the Enteromorpha under natural conditions, and air-dry the Enteromorpha enteromorpha is pulverized using a colloid mill to obtain seaweed particles with a particle size of 300 meshes;

[0027] S2. Dissolving the seaweed particles with distilled water, stirring continuously during the dissolving process, and keeping the water temperature at 37°C during dissolution, to obtain a seaweed colloid solution;

[0028] S3. Enzymolysis: Add cellulase with a final concentration of 1.5 g / L to the seaweed colloid solution, and mix the seaweed colloid solution continuously. After stirring for 4 hours, continue to add β-glucose with a final concentration of 1.8 g / L. Glycanase, g...

Embodiment 2

[0034] A kind of seaweed fertilizer preparation method using Enteromorpha as raw material comprises the following steps:

[0035] S1. Clean the fresh Enteromorpha with tap water for 3 times, soak the cleaned Enteromorpha in 95% ethanol for 42 h, take out the Enteromorpha and wash it with distilled water for 3 times, air-dry the Enteromorpha under natural conditions, and air-dry the Enteromorpha enteromorpha is pulverized using a colloid mill to obtain seaweed particles with a particle size of 300 meshes;

[0036] S2. Dissolving the seaweed particles with distilled water, stirring continuously during the dissolving process, and keeping the water temperature at 37°C during dissolution, to obtain a seaweed colloid solution;

[0037] S3. Enzymatic hydrolysis: Add cellulase with a final concentration of 1.8 g / L to the seaweed colloid solution, stir and mix the seaweed colloid solution continuously, after stirring for 5 hours, continue to add β-glucose with a final concentration of ...

Embodiment 3

[0043] A kind of seaweed fertilizer preparation method using Enteromorpha as raw material comprises the following steps:

[0044] S1. Clean the fresh Enteromorpha with tap water for 3 times, soak the cleaned Enteromorpha in 95% ethanol for 42 h, take out the Enteromorpha and wash it with distilled water for 3 times, air-dry the Enteromorpha under natural conditions, and air-dry the Enteromorpha enteromorpha is pulverized using a colloid mill to obtain seaweed particles with a particle size of 260-300 mesh;

[0045] S2. Dissolving the seaweed particles with distilled water, stirring continuously during the dissolving process, and keeping the water temperature at 37°C during dissolution, to obtain a seaweed colloid solution;

[0046] S3. Enzymatic hydrolysis: Add cellulase with a final concentration of 2.0 g / L to the seaweed colloid solution, stir and mix the seaweed colloid solution continuously, after stirring for 6 hours, continue to add β-glucose enzyme with a final concentr...