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Fluorescent probe for dopamine determination and preparation method and application thereof

A fluorescent probe, dopamine technology, applied in the field of fluorescent probes, can solve the problems of narrow detection range, complex, expensive instruments and equipment

Inactive Publication Date: 2018-08-17
CHINA WEST NORMAL UNIVERSITY
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Problems solved by technology

It is undeniable that electrochemistry is a sensitive and fast analytical method, but the tedious and complicated electrode preparation process is also obvious to all
Raman spectroscopy has good sensitivity and selectivity, but requires expensive equipment and is not suitable for routine detection
Colorimetry is a simple and fast analytical method, but its detection range is narrow and its accuracy is low

Method used

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  • Fluorescent probe for dopamine determination and preparation method and application thereof
  • Fluorescent probe for dopamine determination and preparation method and application thereof
  • Fluorescent probe for dopamine determination and preparation method and application thereof

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Embodiment Construction

[0019] 1. Synthesis of fluorescent carbon quantum dots

[0020] In this experiment, L-arginine was used as the carbon source, and fluorescent carbon quantum dots were synthesized in one step by hydrothermal method. The specific steps are as follows: First, weigh 0.10g of arginine, add 20mL of ultrapure water to dissolve, mix well and transfer to 50mL In the reaction kettle, react at 180°C for 6h, after cooling to room temperature naturally, pass the reaction solution through a 0.22 μm microporous filter membrane, the filtrate is the synthetic fluorescent carbon quantum dot solution, and store it in a refrigerator at 4°C for later use.

[0021] Investigation on Synthesis Conditions of Carbon Quantum Dots

[0022] 1.1 Investigation of reactant dosage ratio

[0023] In order to study the effect of the amount of carbon source and reaction medium on the fluorescence properties of the synthesized C-dots during the synthesis process, the quality of the carbon source arginine was fix...

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Abstract

The invention discloses a fluorescent probe for dopamine determination. The fluorescent probe for the dopamine determination is characterized in that with L-arginine as a carbon source, one-step synthesis of carbon quantum dots with strong fluorescence emission is achieved through a hydrothermal method; the carbon quantum dots are characterized by such things as ultraviolet absorption spectrum (UV), fluorescence spectrum (PL), fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and fluorescent properties of the synthetic carbon quantum dots and the fact that the surface of the same contains a large number of functional groups such as hydroxyl groups and carbonyl groups are confirmed; according to the fluorescence quenching effect of dopamine on C-dots(carbon dots), the C-dots are used as a fluorescent probe for detecting the dopamine, and experimental conditions such as pH (hydrogen ion concentration), carbon point usage, reaction temperature andtime are optimized; a quantitative detection method for the dopamine is established under the optimal detection conditions, and the detection limit is as low as 93 nanometers, which proves that the method can be used for sensitive detection of dopamine in actual samples.

Description

technical field [0001] The invention relates to the technical field of fluorescent probes, in particular to a fluorescent probe for measuring dopamine and its preparation method and application. Background technique [0002] Dopamine is 4-(2-aminoethyl)-1,2-benzenediol, and its structure is as follows: [0003] [0004] Dopamine is a neurotransmitter that plays a very important role in the central nervous system and peripheral nervous system. Studies have shown that abnormal levels of dopamine will lead to schizophrenia, Alzheimer's and Parkinson's disease. Dopamine hydrochloride is the hydrochloride form of dopamine. Clinically, dopamine hydrochloride injection is often used to treat shock caused by major psychological trauma, cardiac surgery, heart failure, etc. It is a widely used booster drug. Therefore, it is very necessary to find a simple, economical and sensitive method for detecting the content of dopamine in clinical drugs. [0005] At present, a large number...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 杨秀培杨正丽许华刘明显廖祥军
Owner CHINA WEST NORMAL UNIVERSITY
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